Platelet-activating factor antagonist WEB-2170 inhibits lipopolysaccharide-induced, but not antiprogestin-induced, preterm cervical ripening in timed-pregnant rats

Holger Maul, Leili Shi, Stephen G. Marx, Robert E. Garfield, George Saade

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OBJECTIVE: The purpose of this study was to determine whether the platelet-activating factor antagonist WEB-2170 inhibits preterm cervical ripening induced by lipopolysaccharide or by antiprogestin RU 486. STUDY DESIGN: Timed-pregnant rats were killed on day 16 after treatment with (1) WEB-2170, lipopolysaccharide, lipopolysaccharide plus WEB-2170, or vehicle control and (2) with WEB-2170, RU 486, RU 486 plus WEB-2170, or vehicle control. Cervical ripening was assessed by light-induced fluorescence and resistance to stretch. Statistics were assessed by 1 -way analysis of variance followed by Tukey-test (P < .05). RESULTS: Light-induced fluorescence and resistance to stretch were significantly lower in the lipopolysaccharide-treated and in the RU486-treated animals compared with vehicle control (lipopolysaccharide:light- induced fluorescence, 7.0 ± 0.6 vs 12.8 ± 0.8 [P = .001]; resistance to stretch, 0.41 ± 0.03 N/mm vs 0.54 ± 0.04 N/mm [P < .05]; RU486:light-induced fluorescence, 9.6 ± 0.6 vs 11.7 ± 0.6 [P < .05]; resistance to stretch, 0.28 ± 0.06 N/mm vs 0.61 ± 0.02 N/mm [P < .001]). Compared with vehicle control, WEB-2170 alone did not alter cervical light-induced fluorescence or resistance to stretch. Although WEB-2170 significantly blocked cervical ripening after lipopolysaccharide administration (light-induced fluorescence; 11.3 ± 1. 3 [P < .05]; resistance to stretch, 0.61 ± 0.04 [P < .01]), WEB-2170 did not inhibit the RU 486-induced cervical ripening. CONCLUSION: Although infection-related cervical ripening is inhibited by platelet-activating factor antagonists, the physiologic process of cervical ripening appears to be unaffected. Platelet-activating factor inhibition may be of clinical value in the infection-related pathologic processes that are responsible for premature cervical ripening.

Original languageEnglish (US)
Pages (from-to)963-967
Number of pages5
JournalAmerican Journal of Obstetrics and Gynecology
Volume189
Issue number4
DOIs
StatePublished - Oct 2003

Fingerprint

Cervical Ripening
Platelet Activating Factor
Lipopolysaccharides
Mifepristone
Fluorescence
Light
bepafant
Pathologic Processes
Infection
Analysis of Variance

Keywords

  • Light-induced fluorescence
  • Parturition
  • Platelet-activating factor
  • Uterine cervix

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Platelet-activating factor antagonist WEB-2170 inhibits lipopolysaccharide-induced, but not antiprogestin-induced, preterm cervical ripening in timed-pregnant rats. / Maul, Holger; Shi, Leili; Marx, Stephen G.; Garfield, Robert E.; Saade, George.

In: American Journal of Obstetrics and Gynecology, Vol. 189, No. 4, 10.2003, p. 963-967.

Research output: Contribution to journalArticle

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title = "Platelet-activating factor antagonist WEB-2170 inhibits lipopolysaccharide-induced, but not antiprogestin-induced, preterm cervical ripening in timed-pregnant rats",
abstract = "OBJECTIVE: The purpose of this study was to determine whether the platelet-activating factor antagonist WEB-2170 inhibits preterm cervical ripening induced by lipopolysaccharide or by antiprogestin RU 486. STUDY DESIGN: Timed-pregnant rats were killed on day 16 after treatment with (1) WEB-2170, lipopolysaccharide, lipopolysaccharide plus WEB-2170, or vehicle control and (2) with WEB-2170, RU 486, RU 486 plus WEB-2170, or vehicle control. Cervical ripening was assessed by light-induced fluorescence and resistance to stretch. Statistics were assessed by 1 -way analysis of variance followed by Tukey-test (P < .05). RESULTS: Light-induced fluorescence and resistance to stretch were significantly lower in the lipopolysaccharide-treated and in the RU486-treated animals compared with vehicle control (lipopolysaccharide:light- induced fluorescence, 7.0 ± 0.6 vs 12.8 ± 0.8 [P = .001]; resistance to stretch, 0.41 ± 0.03 N/mm vs 0.54 ± 0.04 N/mm [P < .05]; RU486:light-induced fluorescence, 9.6 ± 0.6 vs 11.7 ± 0.6 [P < .05]; resistance to stretch, 0.28 ± 0.06 N/mm vs 0.61 ± 0.02 N/mm [P < .001]). Compared with vehicle control, WEB-2170 alone did not alter cervical light-induced fluorescence or resistance to stretch. Although WEB-2170 significantly blocked cervical ripening after lipopolysaccharide administration (light-induced fluorescence; 11.3 ± 1. 3 [P < .05]; resistance to stretch, 0.61 ± 0.04 [P < .01]), WEB-2170 did not inhibit the RU 486-induced cervical ripening. CONCLUSION: Although infection-related cervical ripening is inhibited by platelet-activating factor antagonists, the physiologic process of cervical ripening appears to be unaffected. Platelet-activating factor inhibition may be of clinical value in the infection-related pathologic processes that are responsible for premature cervical ripening.",
keywords = "Light-induced fluorescence, Parturition, Platelet-activating factor, Uterine cervix",
author = "Holger Maul and Leili Shi and Marx, {Stephen G.} and Garfield, {Robert E.} and George Saade",
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T1 - Platelet-activating factor antagonist WEB-2170 inhibits lipopolysaccharide-induced, but not antiprogestin-induced, preterm cervical ripening in timed-pregnant rats

AU - Maul, Holger

AU - Shi, Leili

AU - Marx, Stephen G.

AU - Garfield, Robert E.

AU - Saade, George

PY - 2003/10

Y1 - 2003/10

N2 - OBJECTIVE: The purpose of this study was to determine whether the platelet-activating factor antagonist WEB-2170 inhibits preterm cervical ripening induced by lipopolysaccharide or by antiprogestin RU 486. STUDY DESIGN: Timed-pregnant rats were killed on day 16 after treatment with (1) WEB-2170, lipopolysaccharide, lipopolysaccharide plus WEB-2170, or vehicle control and (2) with WEB-2170, RU 486, RU 486 plus WEB-2170, or vehicle control. Cervical ripening was assessed by light-induced fluorescence and resistance to stretch. Statistics were assessed by 1 -way analysis of variance followed by Tukey-test (P < .05). RESULTS: Light-induced fluorescence and resistance to stretch were significantly lower in the lipopolysaccharide-treated and in the RU486-treated animals compared with vehicle control (lipopolysaccharide:light- induced fluorescence, 7.0 ± 0.6 vs 12.8 ± 0.8 [P = .001]; resistance to stretch, 0.41 ± 0.03 N/mm vs 0.54 ± 0.04 N/mm [P < .05]; RU486:light-induced fluorescence, 9.6 ± 0.6 vs 11.7 ± 0.6 [P < .05]; resistance to stretch, 0.28 ± 0.06 N/mm vs 0.61 ± 0.02 N/mm [P < .001]). Compared with vehicle control, WEB-2170 alone did not alter cervical light-induced fluorescence or resistance to stretch. Although WEB-2170 significantly blocked cervical ripening after lipopolysaccharide administration (light-induced fluorescence; 11.3 ± 1. 3 [P < .05]; resistance to stretch, 0.61 ± 0.04 [P < .01]), WEB-2170 did not inhibit the RU 486-induced cervical ripening. CONCLUSION: Although infection-related cervical ripening is inhibited by platelet-activating factor antagonists, the physiologic process of cervical ripening appears to be unaffected. Platelet-activating factor inhibition may be of clinical value in the infection-related pathologic processes that are responsible for premature cervical ripening.

AB - OBJECTIVE: The purpose of this study was to determine whether the platelet-activating factor antagonist WEB-2170 inhibits preterm cervical ripening induced by lipopolysaccharide or by antiprogestin RU 486. STUDY DESIGN: Timed-pregnant rats were killed on day 16 after treatment with (1) WEB-2170, lipopolysaccharide, lipopolysaccharide plus WEB-2170, or vehicle control and (2) with WEB-2170, RU 486, RU 486 plus WEB-2170, or vehicle control. Cervical ripening was assessed by light-induced fluorescence and resistance to stretch. Statistics were assessed by 1 -way analysis of variance followed by Tukey-test (P < .05). RESULTS: Light-induced fluorescence and resistance to stretch were significantly lower in the lipopolysaccharide-treated and in the RU486-treated animals compared with vehicle control (lipopolysaccharide:light- induced fluorescence, 7.0 ± 0.6 vs 12.8 ± 0.8 [P = .001]; resistance to stretch, 0.41 ± 0.03 N/mm vs 0.54 ± 0.04 N/mm [P < .05]; RU486:light-induced fluorescence, 9.6 ± 0.6 vs 11.7 ± 0.6 [P < .05]; resistance to stretch, 0.28 ± 0.06 N/mm vs 0.61 ± 0.02 N/mm [P < .001]). Compared with vehicle control, WEB-2170 alone did not alter cervical light-induced fluorescence or resistance to stretch. Although WEB-2170 significantly blocked cervical ripening after lipopolysaccharide administration (light-induced fluorescence; 11.3 ± 1. 3 [P < .05]; resistance to stretch, 0.61 ± 0.04 [P < .01]), WEB-2170 did not inhibit the RU 486-induced cervical ripening. CONCLUSION: Although infection-related cervical ripening is inhibited by platelet-activating factor antagonists, the physiologic process of cervical ripening appears to be unaffected. Platelet-activating factor inhibition may be of clinical value in the infection-related pathologic processes that are responsible for premature cervical ripening.

KW - Light-induced fluorescence

KW - Parturition

KW - Platelet-activating factor

KW - Uterine cervix

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