Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens

V. A. Feodorova, L. N. Pan'kina, E. P. Savostina, O. S. Kuznetsov, N. P. Konnov, L. V. Sayapina, S. V. Dentovskaya, R. Z. Shaikhutdinova, S. A. Ageev, B. Lindner, A. N. Kondakova, O. V. Bystrova, N. A. Kocharova, S. N. Senchenkova, O. Holst, G. B. Pier, Y. A. Knirel, A. P. Anisimov, Vladimir Motin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Deletion mutants in the lpxM gene in two Yersinia pestis strains, the live Russian vaccine strain EV NIIEG and a fully virulent strain, 231, synthesise a less toxic penta-acylated lipopolysaccharide (LPS). Analysis of these mutants revealed they possessed marked reductions in expression and immunoreactivity of numerous major proteins and carbohydrate antigens, including F1, Pla, Ymt, V antigen, LPS, and ECA. Moreover, both mutants demonstrated altered epitope specificities of the antigens as determined in immunodot-ELISAs and immunoblotting analyses using a panel of monoclonal antibodies. The strains also differed in their susceptibility to the diagnostic plague bacteriophage L-413C. These findings indicate that the effects of the lpxM mutation on reduced virulence and enhanced immunity of the Y. pestis EV ΔlpxM is also associated with these pleiotropic changes and not just to changes in the lipid A acylation.

Original languageEnglish
Pages (from-to)2240-2250
Number of pages11
JournalVaccine
Volume27
Issue number16
DOIs
StatePublished - Apr 6 2009

Fingerprint

Yersinia pestis
Lipopolysaccharides
biosynthesis
antigens
mutation
Antigens
Lipid A
mutants
lipopolysaccharides
Rubiaceae
Mutation
Plague
Acylation
Poisons
Immunoblotting
Bacteriophages
Virulence
Epitopes
Immunity
Pentas

Keywords

  • Live plague vaccine
  • lpxM(msbB) mutant
  • Plague immunity
  • Virulence
  • Yersinia pestis 231
  • Yersinia pestis antigens
  • Yersinia pestis EV NIIEG

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens. / Feodorova, V. A.; Pan'kina, L. N.; Savostina, E. P.; Kuznetsov, O. S.; Konnov, N. P.; Sayapina, L. V.; Dentovskaya, S. V.; Shaikhutdinova, R. Z.; Ageev, S. A.; Lindner, B.; Kondakova, A. N.; Bystrova, O. V.; Kocharova, N. A.; Senchenkova, S. N.; Holst, O.; Pier, G. B.; Knirel, Y. A.; Anisimov, A. P.; Motin, Vladimir.

In: Vaccine, Vol. 27, No. 16, 06.04.2009, p. 2240-2250.

Research output: Contribution to journalArticle

Feodorova, VA, Pan'kina, LN, Savostina, EP, Kuznetsov, OS, Konnov, NP, Sayapina, LV, Dentovskaya, SV, Shaikhutdinova, RZ, Ageev, SA, Lindner, B, Kondakova, AN, Bystrova, OV, Kocharova, NA, Senchenkova, SN, Holst, O, Pier, GB, Knirel, YA, Anisimov, AP & Motin, V 2009, 'Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens', Vaccine, vol. 27, no. 16, pp. 2240-2250. https://doi.org/10.1016/j.vaccine.2009.02.020
Feodorova, V. A. ; Pan'kina, L. N. ; Savostina, E. P. ; Kuznetsov, O. S. ; Konnov, N. P. ; Sayapina, L. V. ; Dentovskaya, S. V. ; Shaikhutdinova, R. Z. ; Ageev, S. A. ; Lindner, B. ; Kondakova, A. N. ; Bystrova, O. V. ; Kocharova, N. A. ; Senchenkova, S. N. ; Holst, O. ; Pier, G. B. ; Knirel, Y. A. ; Anisimov, A. P. ; Motin, Vladimir. / Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens. In: Vaccine. 2009 ; Vol. 27, No. 16. pp. 2240-2250.
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abstract = "Deletion mutants in the lpxM gene in two Yersinia pestis strains, the live Russian vaccine strain EV NIIEG and a fully virulent strain, 231, synthesise a less toxic penta-acylated lipopolysaccharide (LPS). Analysis of these mutants revealed they possessed marked reductions in expression and immunoreactivity of numerous major proteins and carbohydrate antigens, including F1, Pla, Ymt, V antigen, LPS, and ECA. Moreover, both mutants demonstrated altered epitope specificities of the antigens as determined in immunodot-ELISAs and immunoblotting analyses using a panel of monoclonal antibodies. The strains also differed in their susceptibility to the diagnostic plague bacteriophage L-413C. These findings indicate that the effects of the lpxM mutation on reduced virulence and enhanced immunity of the Y. pestis EV ΔlpxM is also associated with these pleiotropic changes and not just to changes in the lipid A acylation.",
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AU - Feodorova, V. A.

AU - Pan'kina, L. N.

AU - Savostina, E. P.

AU - Kuznetsov, O. S.

AU - Konnov, N. P.

AU - Sayapina, L. V.

AU - Dentovskaya, S. V.

AU - Shaikhutdinova, R. Z.

AU - Ageev, S. A.

AU - Lindner, B.

AU - Kondakova, A. N.

AU - Bystrova, O. V.

AU - Kocharova, N. A.

AU - Senchenkova, S. N.

AU - Holst, O.

AU - Pier, G. B.

AU - Knirel, Y. A.

AU - Anisimov, A. P.

AU - Motin, Vladimir

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