Polarized expression of CD74 by gastric epithelial cells

Carlos A. Barrera, Ellen J. Beswick, Johanna C. Sierra, David Bland, Rosario Espejo, Randy Mifflin, Patrick Adegboyega, Sheila E. Crowe, Peter B. Ernst, Victor Reyes

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

CD74 is known as the major histocompatibility complex (MHC) class II-associated invariant chain (II) that regulates the cell biology and functions of MHC class II molecules. Class II MHC and II expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules. Given the importance of Ii in the biology of class II MHC, we sought to examine the expression of II by gastric epithelial cells (GEC) to determine whether class II MHC molecules in these nonconventional APC cells were under the control of II and to further support the role that these cells may play in local immune and inflammatory responses during Helicobacter pylori infection. Thus we examined the expression of II on GEC from human biopsy samples and then confirmed this observation using independent methods on several GEC lines. The mRNA for II was detected by RT-PCR, and the various protein isoforms were also detected. Interestingly, these cells have a high level expression of surface II, which is polarized to the apical surface. These studies are the first to demonstrate the constitutive expression of II by human GEC.

Original languageEnglish (US)
Pages (from-to)1481-1489
Number of pages9
JournalJournal of Histochemistry and Cytochemistry
Volume53
Issue number12
DOIs
StatePublished - Dec 2005

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Stomach
Epithelial Cells
Major Histocompatibility Complex
Antigen-Presenting Cells
invariant chain
Helicobacter Infections
Helicobacter pylori
Cell Biology
Protein Isoforms
Inflammation
Biopsy
Cell Line
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Antigen presentation
  • CD74
  • Invariant chain
  • Mucosal immunity

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Barrera, C. A., Beswick, E. J., Sierra, J. C., Bland, D., Espejo, R., Mifflin, R., ... Reyes, V. (2005). Polarized expression of CD74 by gastric epithelial cells. Journal of Histochemistry and Cytochemistry, 53(12), 1481-1489. https://doi.org/10.1369/jhc.4A6552.2005

Polarized expression of CD74 by gastric epithelial cells. / Barrera, Carlos A.; Beswick, Ellen J.; Sierra, Johanna C.; Bland, David; Espejo, Rosario; Mifflin, Randy; Adegboyega, Patrick; Crowe, Sheila E.; Ernst, Peter B.; Reyes, Victor.

In: Journal of Histochemistry and Cytochemistry, Vol. 53, No. 12, 12.2005, p. 1481-1489.

Research output: Contribution to journalArticle

Barrera, CA, Beswick, EJ, Sierra, JC, Bland, D, Espejo, R, Mifflin, R, Adegboyega, P, Crowe, SE, Ernst, PB & Reyes, V 2005, 'Polarized expression of CD74 by gastric epithelial cells', Journal of Histochemistry and Cytochemistry, vol. 53, no. 12, pp. 1481-1489. https://doi.org/10.1369/jhc.4A6552.2005
Barrera CA, Beswick EJ, Sierra JC, Bland D, Espejo R, Mifflin R et al. Polarized expression of CD74 by gastric epithelial cells. Journal of Histochemistry and Cytochemistry. 2005 Dec;53(12):1481-1489. https://doi.org/10.1369/jhc.4A6552.2005
Barrera, Carlos A. ; Beswick, Ellen J. ; Sierra, Johanna C. ; Bland, David ; Espejo, Rosario ; Mifflin, Randy ; Adegboyega, Patrick ; Crowe, Sheila E. ; Ernst, Peter B. ; Reyes, Victor. / Polarized expression of CD74 by gastric epithelial cells. In: Journal of Histochemistry and Cytochemistry. 2005 ; Vol. 53, No. 12. pp. 1481-1489.
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