Polarized expression of the GFP-tagged rat V1a vasopressin receptor

Danae M. Campos; Carlos E. Reyes; Jose Sarmiento; Javier Navarro; Carlos B. González

doi:10.1006/bbrc.2001.5981

Polarized expression of the GFP-tagged rat V_1a vasopressin receptor

Danae M. Campos, Carlos E. Reyes, Jose Sarmiento, Javier Navarro, Carlos B. González

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We investigated the targeting of the V_1a receptor fused with the green fluorescence protein (V_1aR-GFP) in polarized MDCK cells. Cells expressing V_1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V_1aR, V_1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V_1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V_1a receptors.

Original language	English (US)
Pages (from-to)	325-328
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	289
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.2001.5981
State	Published - Nov 30 2001

Keywords

Polarized expression
Signaling mechanism
V receptor
Vasopressin

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.2001.5981

Cite this

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title = "Polarized expression of the GFP-tagged rat V1a vasopressin receptor",

abstract = "We investigated the targeting of the V1a receptor fused with the green fluorescence protein (V1aR-GFP) in polarized MDCK cells. Cells expressing V1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V1aR, V1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V1a receptors.",

keywords = "Polarized expression, Signaling mechanism, V receptor, Vasopressin",

author = "Campos, {Danae M.} and Reyes, {Carlos E.} and Jose Sarmiento and Javier Navarro and Gonz{\'a}lez, {Carlos B.}",

note = "Funding Information: We thank the expert technical assistant of E. Oyarzun. This work is supported by Grant 8980002 from Fondecyt and DIUAH. The laser confocal microscope was purchased with a grant to the CECS from the Andes Foundation. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2001",

month = nov,

day = "30",

doi = "10.1006/bbrc.2001.5981",

language = "English (US)",

volume = "289",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Polarized expression of the GFP-tagged rat V1a vasopressin receptor

AU - Campos, Danae M.

AU - Reyes, Carlos E.

AU - Sarmiento, Jose

AU - Navarro, Javier

AU - González, Carlos B.

N1 - Funding Information: We thank the expert technical assistant of E. Oyarzun. This work is supported by Grant 8980002 from Fondecyt and DIUAH. The laser confocal microscope was purchased with a grant to the CECS from the Andes Foundation. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2001/11/30

Y1 - 2001/11/30

N2 - We investigated the targeting of the V1a receptor fused with the green fluorescence protein (V1aR-GFP) in polarized MDCK cells. Cells expressing V1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V1aR, V1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V1a receptors.

AB - We investigated the targeting of the V1a receptor fused with the green fluorescence protein (V1aR-GFP) in polarized MDCK cells. Cells expressing V1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V1aR, V1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V1a receptors.

KW - Polarized expression

KW - Signaling mechanism

KW - V receptor

KW - Vasopressin

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