Polarized expression of the GFP-tagged rat V1a vasopressin receptor

Danae M. Campos, Carlos E. Reyes, Jose Sarmiento, Javier Navarro, Carlos B. González

    Research output: Contribution to journalArticlepeer-review

    4 Scopus citations

    Abstract

    We investigated the targeting of the V1a receptor fused with the green fluorescence protein (V1aR-GFP) in polarized MDCK cells. Cells expressing V1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V1aR, V1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V1a receptors.

    Original languageEnglish (US)
    Pages (from-to)325-328
    Number of pages4
    JournalBiochemical and Biophysical Research Communications
    Volume289
    Issue number2
    DOIs
    StatePublished - Nov 30 2001

    Keywords

    • Polarized expression
    • Signaling mechanism
    • V receptor
    • Vasopressin

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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