Polarized expression of the GFP-tagged rat V1a vasopressin receptor

Danae M. Campos, Carlos E. Reyes, Jose Sarmiento, Javier Navarro, Carlos B. González

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We investigated the targeting of the V1a receptor fused with the green fluorescence protein (V1aR-GFP) in polarized MDCK cells. Cells expressing V1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V1aR, V1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V1a receptors.

Original languageEnglish (US)
Pages (from-to)325-328
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume289
Issue number2
DOIs
StatePublished - Nov 30 2001

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Keywords

  • Polarized expression
  • Signaling mechanism
  • V receptor
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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