Polarized expression of the GFP-tagged rat V1a vasopressin receptor

Danae M. Campos, Carlos E. Reyes, Jose Sarmiento, Javier Navarro, Carlos B. González

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated the targeting of the V1a receptor fused with the green fluorescence protein (V1aR-GFP) in polarized MDCK cells. Cells expressing V1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V1aR, V1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V1a receptors.

Original languageEnglish (US)
Pages (from-to)325-328
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume289
Issue number2
DOIs
StatePublished - Nov 30 2001

Fingerprint

Madin Darby Canine Kidney Cells
Fluorescence
Cells
Confocal microscopy
Vasopressins
Sorting
Protein Binding
Confocal Microscopy
Proteins
Calcium
rat V1a vasopressin receptor

Keywords

  • Polarized expression
  • Signaling mechanism
  • V receptor
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Polarized expression of the GFP-tagged rat V1a vasopressin receptor. / Campos, Danae M.; Reyes, Carlos E.; Sarmiento, Jose; Navarro, Javier; González, Carlos B.

In: Biochemical and Biophysical Research Communications, Vol. 289, No. 2, 30.11.2001, p. 325-328.

Research output: Contribution to journalArticle

Campos, Danae M. ; Reyes, Carlos E. ; Sarmiento, Jose ; Navarro, Javier ; González, Carlos B. / Polarized expression of the GFP-tagged rat V1a vasopressin receptor. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 289, No. 2. pp. 325-328.
@article{50bbaa93f83b4630a91579a05689d9e4,
title = "Polarized expression of the GFP-tagged rat V1a vasopressin receptor",
abstract = "We investigated the targeting of the V1a receptor fused with the green fluorescence protein (V1aR-GFP) in polarized MDCK cells. Cells expressing V1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V1aR, V1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V1a receptors.",
keywords = "Polarized expression, Signaling mechanism, V receptor, Vasopressin",
author = "Campos, {Danae M.} and Reyes, {Carlos E.} and Jose Sarmiento and Javier Navarro and Gonz{\'a}lez, {Carlos B.}",
year = "2001",
month = "11",
day = "30",
doi = "10.1006/bbrc.2001.5981",
language = "English (US)",
volume = "289",
pages = "325--328",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Polarized expression of the GFP-tagged rat V1a vasopressin receptor

AU - Campos, Danae M.

AU - Reyes, Carlos E.

AU - Sarmiento, Jose

AU - Navarro, Javier

AU - González, Carlos B.

PY - 2001/11/30

Y1 - 2001/11/30

N2 - We investigated the targeting of the V1a receptor fused with the green fluorescence protein (V1aR-GFP) in polarized MDCK cells. Cells expressing V1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V1aR, V1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V1a receptors.

AB - We investigated the targeting of the V1a receptor fused with the green fluorescence protein (V1aR-GFP) in polarized MDCK cells. Cells expressing V1aR-GFP displayed binding to vasopressin (AVP) and AVP induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V1aR, V1aR-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V1a receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V1a receptors.

KW - Polarized expression

KW - Signaling mechanism

KW - V receptor

KW - Vasopressin

UR - http://www.scopus.com/inward/record.url?scp=0035976789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035976789&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2001.5981

DO - 10.1006/bbrc.2001.5981

M3 - Article

VL - 289

SP - 325

EP - 328

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -