Pollen-induced oxidative stress influences both innate and adaptive immune responses via altering dendritic cell functions

Aniko Csillag, Istvan Boldogh, Kitti Pazmandi, Zoltan Magyarics, Peter Gogolak, Sanjiv Sur, Eva Rajnavolgyi, Attila Bacsi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

It has been demonstrated that pollen grains contain NAD(P)H oxidases that induce oxidative stress in the airways, and this oxidative insult is critical for the development of allergic inflammation in sensitized mice. On the basis of this observation, we have examined whether pollen grain exposure triggers oxidative stress in dendritic cells (DCs), altering their functions. To test this hypothesis, human monocyte-derived DCs were treated with ragweed pollen grains. Our findings show that exposure to pollen grains induces an increase in the intracellular levels of reactive oxygen species in DCs. Our data also indicate that besides the NAD(P)H oxidases, other component(s) of pollen grains contributes to this phenomenon. Elevated levels of intracellular reactive oxygen species triggered the production of IL-8 as well as proinflammatory cytokines, such as TNF-α and IL-6. Treatment with pollen grains initiated the maturation of DCs, strongly upregulated the membrane expression of CD80, CD86, CD83, and HLA-DR, and caused only a slight increase in the expression of CD40. The pollen-treated DCs induced the development of naive T lymphocytes toward effector T cells with a mixed profile of cytokine production. Antioxidant inhibited both the phenotypic and functional changes of DCs, underlining the importance of oxidative stress in these processes. Collectively, these data show that pollen exposure-induced oxidative stress may contribute to local innate immunity and participate in the initiation of adaptive immune responses to pollen Ags.

Original languageEnglish (US)
Pages (from-to)2377-2385
Number of pages9
JournalJournal of Immunology
Volume184
Issue number5
DOIs
StatePublished - Mar 1 2010

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Adaptive Immunity
Pollen
Innate Immunity
Dendritic Cells
Oxidative Stress
NADPH Oxidase
Reactive Oxygen Species
Cytokines
T-Lymphocytes
HLA-DR Antigens
Interleukin-8
Monocytes
Interleukin-6
Antioxidants
Inflammation
Membranes

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Pollen-induced oxidative stress influences both innate and adaptive immune responses via altering dendritic cell functions. / Csillag, Aniko; Boldogh, Istvan; Pazmandi, Kitti; Magyarics, Zoltan; Gogolak, Peter; Sur, Sanjiv; Rajnavolgyi, Eva; Bacsi, Attila.

In: Journal of Immunology, Vol. 184, No. 5, 01.03.2010, p. 2377-2385.

Research output: Contribution to journalArticle

Csillag, Aniko ; Boldogh, Istvan ; Pazmandi, Kitti ; Magyarics, Zoltan ; Gogolak, Peter ; Sur, Sanjiv ; Rajnavolgyi, Eva ; Bacsi, Attila. / Pollen-induced oxidative stress influences both innate and adaptive immune responses via altering dendritic cell functions. In: Journal of Immunology. 2010 ; Vol. 184, No. 5. pp. 2377-2385.
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