Poly(ADP-ribose) polymerase 1 interacts with nuclear respiratory factor 1 (NRF-1) and plays a role in NRF-1 transcriptional regulation

Mohammad B. Hossain, Ping Ji, Ramakrishnan Anish, Raymond H. Jacobson, Shinako Takada

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Nuclear respiratory factor 1 (NRF-1) is one of the key transcriptional activators for nuclear-coded genes involved in mitochondrial biogenesis and function as well as for many housekeeping genes. A transcriptional co-activator PGC-1 and its related family member PRC have previously been shown to interact with NRF-1 and co-activate NRF-1. We show here that NRF-1 can also directly interact with poly(ADP-ribose) polymerase 1 (PARP-1) and co-purify the PARP-1·DNA-PK ·Ku80·Ku70·topoisomerase IIβ-containing protein complex. Our in vitro binding experiments show that DNA-binding/ dimerization domain of NRF-1 and the N-terminal half of PARP-1, which contains two Zinc fingers and the auto-modification domain, are responsible for the interaction, and that this interaction occurs with or without PARP-1 poly(ADP-ribosyl)ation (PARylation). DNA-bound NRF-1 can form a complex with PARP-1, suggesting that NRF-1 can recruit the PARP-1·DNA-PK ·Ku80·Ku70·topoisomerase IIβ-containing protein complex to the promoter. PARP-1 can also PARylate the DNA-binding domain of NRF-1 and negatively regulate NRF-1·PARP-1 interaction. Transient transfection and chromatin immunoprecipitation experiments suggest that PARP-1 plays a role during transcriptional activation by NRF-1. Our finding identifies a new aspect of transcriptional regulation used by NRF-1.

Original languageEnglish (US)
Pages (from-to)8621-8632
Number of pages12
JournalJournal of Biological Chemistry
Volume284
Issue number13
DOIs
StatePublished - Mar 27 2009
Externally publishedYes

Fingerprint

Nuclear Respiratory Factor 1
Poly(ADP-ribose) Polymerases
DNA
Genes
Poly (ADP-Ribose) Polymerase-1
Dimerization
Chromatin Immunoprecipitation
Essential Genes
Zinc Fingers
Organelle Biogenesis
Adenosine Diphosphate
Transcriptional Activation
Chromatin
Transfection
Zinc

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Poly(ADP-ribose) polymerase 1 interacts with nuclear respiratory factor 1 (NRF-1) and plays a role in NRF-1 transcriptional regulation. / Hossain, Mohammad B.; Ji, Ping; Anish, Ramakrishnan; Jacobson, Raymond H.; Takada, Shinako.

In: Journal of Biological Chemistry, Vol. 284, No. 13, 27.03.2009, p. 8621-8632.

Research output: Contribution to journalArticle

Hossain, Mohammad B. ; Ji, Ping ; Anish, Ramakrishnan ; Jacobson, Raymond H. ; Takada, Shinako. / Poly(ADP-ribose) polymerase 1 interacts with nuclear respiratory factor 1 (NRF-1) and plays a role in NRF-1 transcriptional regulation. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 13. pp. 8621-8632.
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