Poly(ADP-ribose) polymerase inhibition improves endothelial dysfunction induced by hypochlorite

Tamás Radovits, Julia Zotkina, Li Ni Lin, Timo Bömicke, Rawa Arif, Domokos Gero, Eszter M. Horváth, Matthias Karck, Csaba Szabo, Gábor Szabó

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Reactive oxygen species, such as myeloperoxidase-derived hypochlorite, induce oxidative stress and DNA injury. The subsequent activation of the DNA-damage-poly(ADP-ribose) polymerase (PARP) pathway has been implicated in the pathogenesis of various diseases, including ischemia-reperfusion injury, circulatory shock, diabetic complications, and atherosclerosis. We investigated the effect of PARP inhibition on the impaired endothelium-dependent vasorelaxation induced by hypochlorite. In organ bath experiments for isometric tension, we investigated the endothelium-dependent and endotheliumindependent vasorelaxation of isolated rat aortic rings using cumulative concentrations of acetylcholine and sodium nitroprusside. Endothelial dysfunction was induced by exposing rings to hypochlorite (100-400 μM). In the treatment group, rings were preincubated with the PARP inhibitor INO-1001. DNA strand breaks were assessed by the TUNEL method. Immunohistochemistry was performed for 4-hydroxynonenal (a marker of lipid peroxidation), nitrotyrosine (a marker of nitrosative stress), and poly(ADP-ribose) (an enzymatic product of PARP). Exposure to hypochlorite resulted in a dose-dependent impairment of endothelium-dependent vasorelaxation of aortic rings, which was significantly improved by PARP inhibition, whereas the endothelium-independent vasorelaxation remained unaffected. In the hypochlorite groups we found increased DNA breakage, lipidperoxidation, and enhanced nitrotyrosine formation. The hypochloride-induced activation of PARP was prevented by INO-1001. Our results demonstrate that PARP activation contributes to the pathogenesis of hypochlorite-induced endothelial dysfunction, which can be prevented by PARP inhibitors.

Original languageEnglish (US)
Pages (from-to)1204-1212
Number of pages9
JournalExperimental Biology and Medicine
Volume232
Issue number9
DOIs
StatePublished - Oct 2007
Externally publishedYes

Fingerprint

Hypochlorous Acid
Poly(ADP-ribose) Polymerases
Vasodilation
Endothelium
Chemical activation
DNA
DNA Damage
Poly Adenosine Diphosphate Ribose
DNA Breaks
Oxidative stress
In Situ Nick-End Labeling
Nitroprusside
Diabetes Complications
Reperfusion Injury
Baths
Lipid Peroxidation
Peroxidase
Acetylcholine
Rats
Shock

Keywords

  • DNA injury
  • Endothelial dysfunction
  • Hypochlorite
  • Oxidative stress
  • Poly(ADP-ribose) polymerase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Radovits, T., Zotkina, J., Lin, L. N., Bömicke, T., Arif, R., Gero, D., ... Szabó, G. (2007). Poly(ADP-ribose) polymerase inhibition improves endothelial dysfunction induced by hypochlorite. Experimental Biology and Medicine, 232(9), 1204-1212. https://doi.org/10.3181/0701-RM-16

Poly(ADP-ribose) polymerase inhibition improves endothelial dysfunction induced by hypochlorite. / Radovits, Tamás; Zotkina, Julia; Lin, Li Ni; Bömicke, Timo; Arif, Rawa; Gero, Domokos; Horváth, Eszter M.; Karck, Matthias; Szabo, Csaba; Szabó, Gábor.

In: Experimental Biology and Medicine, Vol. 232, No. 9, 10.2007, p. 1204-1212.

Research output: Contribution to journalArticle

Radovits, T, Zotkina, J, Lin, LN, Bömicke, T, Arif, R, Gero, D, Horváth, EM, Karck, M, Szabo, C & Szabó, G 2007, 'Poly(ADP-ribose) polymerase inhibition improves endothelial dysfunction induced by hypochlorite', Experimental Biology and Medicine, vol. 232, no. 9, pp. 1204-1212. https://doi.org/10.3181/0701-RM-16
Radovits, Tamás ; Zotkina, Julia ; Lin, Li Ni ; Bömicke, Timo ; Arif, Rawa ; Gero, Domokos ; Horváth, Eszter M. ; Karck, Matthias ; Szabo, Csaba ; Szabó, Gábor. / Poly(ADP-ribose) polymerase inhibition improves endothelial dysfunction induced by hypochlorite. In: Experimental Biology and Medicine. 2007 ; Vol. 232, No. 9. pp. 1204-1212.
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