Polyamine regulation of atrial natriuretic peptide in cultured cardiocytes

Ulka R. Tipnis; Colin Haile; Paul J. Boor; J. Gutkowska

doi:10.1016/0167-0115(94)90040-X

Polyamine regulation of atrial natriuretic peptide in cultured cardiocytes

Ulka R. Tipnis, Colin Haile, Paul J. Boor, J. Gutkowska

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Abstract

In the following investigation, we have studied the role of polyamines in the regulation of atrial natriuretic peptide (ANP) using ventricular cardiocytes which in culture synthesize and secrete ANP. Polyamines are cellular cations ubiquitous in eukaryotes, and ANP is a hormone synthesized and secreted by the cardiac atria in adult animal. The cardiocytes were isolated from neonatal rat hearts by enzymatic dissociation using trypsin and collagenase. The functional role of polyamines in regulation of ANP was assessed by exposing the cardiocytes to difluoromethylornithine (DFMO) which is an inhibitor of ornithine decarboxylase, an initial rate-limiting enzyme in the biosynthesis of polyamines. The results showed that DFMO reduced the levels of putrescine (diamine) and spermidine (triamine) in cultured cardiocytes, and it decreased the levels of ANP in media and cellular extracts of cardiocytes as a function of its dose. An addition of putrescine (100 μM) restored within 5-15 min the levels of ANP in media of both control and polyamine-depleted cardiocytes. These results suggest that polyamines are one of the cellular factors involved in regulation of ANP secretion in cultured cardiocytes.

Original language	English (US)
Pages (from-to)	75-84
Number of pages	10
Journal	Regulatory Peptides
Volume	52
Issue number	2
DOIs	https://doi.org/10.1016/0167-0115(94)90040-X
State	Published - Jul 14 1994
Externally published	Yes

Keywords

Atrial natriuretic peptide
Cultured cardiocyte
Polyamine
Radioimmunoassay

ASJC Scopus subject areas

Physiology
Biochemistry
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/0167-0115(94)90040-X

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title = "Polyamine regulation of atrial natriuretic peptide in cultured cardiocytes",

abstract = "In the following investigation, we have studied the role of polyamines in the regulation of atrial natriuretic peptide (ANP) using ventricular cardiocytes which in culture synthesize and secrete ANP. Polyamines are cellular cations ubiquitous in eukaryotes, and ANP is a hormone synthesized and secreted by the cardiac atria in adult animal. The cardiocytes were isolated from neonatal rat hearts by enzymatic dissociation using trypsin and collagenase. The functional role of polyamines in regulation of ANP was assessed by exposing the cardiocytes to difluoromethylornithine (DFMO) which is an inhibitor of ornithine decarboxylase, an initial rate-limiting enzyme in the biosynthesis of polyamines. The results showed that DFMO reduced the levels of putrescine (diamine) and spermidine (triamine) in cultured cardiocytes, and it decreased the levels of ANP in media and cellular extracts of cardiocytes as a function of its dose. An addition of putrescine (100 μM) restored within 5-15 min the levels of ANP in media of both control and polyamine-depleted cardiocytes. These results suggest that polyamines are one of the cellular factors involved in regulation of ANP secretion in cultured cardiocytes.",

keywords = "Atrial natriuretic peptide, Cultured cardiocyte, Polyamine, Radioimmunoassay",

author = "Tipnis, {Ulka R.} and Colin Haile and Boor, {Paul J.} and J. Gutkowska",

note = "Funding Information: We thank Maxine Fuller and Loretta Foley for typing of the manuscript and Merrell Dow Research, Cincinnati, OH for providing us with DFMO. The investigation was supported by American Heart (Texas Affiliate No. 92G-615) grant awarded to Dr. Ulka R. Tipnis.",

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AU - Tipnis, Ulka R.

AU - Haile, Colin

AU - Boor, Paul J.

AU - Gutkowska, J.

N1 - Funding Information: We thank Maxine Fuller and Loretta Foley for typing of the manuscript and Merrell Dow Research, Cincinnati, OH for providing us with DFMO. The investigation was supported by American Heart (Texas Affiliate No. 92G-615) grant awarded to Dr. Ulka R. Tipnis.

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N2 - In the following investigation, we have studied the role of polyamines in the regulation of atrial natriuretic peptide (ANP) using ventricular cardiocytes which in culture synthesize and secrete ANP. Polyamines are cellular cations ubiquitous in eukaryotes, and ANP is a hormone synthesized and secreted by the cardiac atria in adult animal. The cardiocytes were isolated from neonatal rat hearts by enzymatic dissociation using trypsin and collagenase. The functional role of polyamines in regulation of ANP was assessed by exposing the cardiocytes to difluoromethylornithine (DFMO) which is an inhibitor of ornithine decarboxylase, an initial rate-limiting enzyme in the biosynthesis of polyamines. The results showed that DFMO reduced the levels of putrescine (diamine) and spermidine (triamine) in cultured cardiocytes, and it decreased the levels of ANP in media and cellular extracts of cardiocytes as a function of its dose. An addition of putrescine (100 μM) restored within 5-15 min the levels of ANP in media of both control and polyamine-depleted cardiocytes. These results suggest that polyamines are one of the cellular factors involved in regulation of ANP secretion in cultured cardiocytes.

AB - In the following investigation, we have studied the role of polyamines in the regulation of atrial natriuretic peptide (ANP) using ventricular cardiocytes which in culture synthesize and secrete ANP. Polyamines are cellular cations ubiquitous in eukaryotes, and ANP is a hormone synthesized and secreted by the cardiac atria in adult animal. The cardiocytes were isolated from neonatal rat hearts by enzymatic dissociation using trypsin and collagenase. The functional role of polyamines in regulation of ANP was assessed by exposing the cardiocytes to difluoromethylornithine (DFMO) which is an inhibitor of ornithine decarboxylase, an initial rate-limiting enzyme in the biosynthesis of polyamines. The results showed that DFMO reduced the levels of putrescine (diamine) and spermidine (triamine) in cultured cardiocytes, and it decreased the levels of ANP in media and cellular extracts of cardiocytes as a function of its dose. An addition of putrescine (100 μM) restored within 5-15 min the levels of ANP in media of both control and polyamine-depleted cardiocytes. These results suggest that polyamines are one of the cellular factors involved in regulation of ANP secretion in cultured cardiocytes.

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Polyamine regulation of atrial natriuretic peptide in cultured cardiocytes

Abstract

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