Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates

Mike J. Loeffelholz; Curt J. Thompson; Dennis D. Gaunt; Frank P. Koontz; Mary J.R. Gilchrist

doi:10.1016/S0732-8893(96)00214-3

Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates

Mike J. Loeffelholz, Curt J. Thompson, Dennis D. Gaunt, Frank P. Koontz, Mary J.R. Gilchrist

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacteriium tuberculosis isolates for which both viable and nonviable cultures were available. DNA extracted from the nonviable cultures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the polymorphic GC-rich repetitive sequence. For both isolates, the nonviable cultures generated PCR-banding patterns identical to those generated by the fresh subcultures.

Original language	English (US)
Pages (from-to)	149-151
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	26
Issue number	3-4
DOIs	https://doi.org/10.1016/S0732-8893(96)00214-3
State	Published - Nov 1996
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/S0732-8893(96)00214-3

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abstract = "Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacteriium tuberculosis isolates for which both viable and nonviable cultures were available. DNA extracted from the nonviable cultures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the polymorphic GC-rich repetitive sequence. For both isolates, the nonviable cultures generated PCR-banding patterns identical to those generated by the fresh subcultures.",

author = "Loeffelholz, {Mike J.} and Thompson, {Curt J.} and Gaunt, {Dennis D.} and Koontz, {Frank P.} and Gilchrist, {Mary J.R.}",

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AU - Thompson, Curt J.

AU - Gaunt, Dennis D.

AU - Koontz, Frank P.

AU - Gilchrist, Mary J.R.

PY - 1996/11

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AB - Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacteriium tuberculosis isolates for which both viable and nonviable cultures were available. DNA extracted from the nonviable cultures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the polymorphic GC-rich repetitive sequence. For both isolates, the nonviable cultures generated PCR-banding patterns identical to those generated by the fresh subcultures.

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Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates

Abstract

ASJC Scopus subject areas

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