Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates

Mike J. Loeffelholz, Curt J. Thompson, Dennis D. Gaunt, Frank P. Koontz, Mary J R Gilchrist

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacteriium tuberculosis isolates for which both viable and nonviable cultures were available. DNA extracted from the nonviable cultures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the polymorphic GC-rich repetitive sequence. For both isolates, the nonviable cultures generated PCR-banding patterns identical to those generated by the fresh subcultures.

Original languageEnglish (US)
Pages (from-to)149-151
Number of pages3
JournalDiagnostic Microbiology and Infectious Disease
Volume26
Issue number3-4
DOIs
StatePublished - Nov 1996
Externally publishedYes

Fingerprint

Mycobacterium tuberculosis
GC Rich Sequence
Polymerase Chain Reaction
Nucleic Acid Repetitive Sequences
Insertional Mutagenesis
Tuberculosis
DNA

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

Loeffelholz, M. J., Thompson, C. J., Gaunt, D. D., Koontz, F. P., & Gilchrist, M. J. R. (1996). Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates. Diagnostic Microbiology and Infectious Disease, 26(3-4), 149-151. https://doi.org/10.1016/S0732-8893(96)00214-3

Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates. / Loeffelholz, Mike J.; Thompson, Curt J.; Gaunt, Dennis D.; Koontz, Frank P.; Gilchrist, Mary J R.

In: Diagnostic Microbiology and Infectious Disease, Vol. 26, No. 3-4, 11.1996, p. 149-151.

Research output: Contribution to journalArticle

Loeffelholz, Mike J. ; Thompson, Curt J. ; Gaunt, Dennis D. ; Koontz, Frank P. ; Gilchrist, Mary J R. / Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates. In: Diagnostic Microbiology and Infectious Disease. 1996 ; Vol. 26, No. 3-4. pp. 149-151.
@article{034a508530a34c78b32d3c2dac925e6f,
title = "Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates",
abstract = "Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacteriium tuberculosis isolates for which both viable and nonviable cultures were available. DNA extracted from the nonviable cultures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the polymorphic GC-rich repetitive sequence. For both isolates, the nonviable cultures generated PCR-banding patterns identical to those generated by the fresh subcultures.",
author = "Loeffelholz, {Mike J.} and Thompson, {Curt J.} and Gaunt, {Dennis D.} and Koontz, {Frank P.} and Gilchrist, {Mary J R}",
year = "1996",
month = "11",
doi = "10.1016/S0732-8893(96)00214-3",
language = "English (US)",
volume = "26",
pages = "149--151",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "3-4",

}

TY - JOUR

T1 - Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates

AU - Loeffelholz, Mike J.

AU - Thompson, Curt J.

AU - Gaunt, Dennis D.

AU - Koontz, Frank P.

AU - Gilchrist, Mary J R

PY - 1996/11

Y1 - 1996/11

N2 - Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacteriium tuberculosis isolates for which both viable and nonviable cultures were available. DNA extracted from the nonviable cultures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the polymorphic GC-rich repetitive sequence. For both isolates, the nonviable cultures generated PCR-banding patterns identical to those generated by the fresh subcultures.

AB - Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacteriium tuberculosis isolates for which both viable and nonviable cultures were available. DNA extracted from the nonviable cultures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the polymorphic GC-rich repetitive sequence. For both isolates, the nonviable cultures generated PCR-banding patterns identical to those generated by the fresh subcultures.

UR - http://www.scopus.com/inward/record.url?scp=0030295160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030295160&partnerID=8YFLogxK

U2 - 10.1016/S0732-8893(96)00214-3

DO - 10.1016/S0732-8893(96)00214-3

M3 - Article

C2 - 9078452

AN - SCOPUS:0030295160

VL - 26

SP - 149

EP - 151

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 3-4

ER -