Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates

Mike J. Loeffelholz, Curt J. Thompson, Dennis D. Gaunt, Frank P. Koontz, Mary J.R. Gilchrist

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacteriium tuberculosis isolates for which both viable and nonviable cultures were available. DNA extracted from the nonviable cultures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the polymorphic GC-rich repetitive sequence. For both isolates, the nonviable cultures generated PCR-banding patterns identical to those generated by the fresh subcultures.

Original languageEnglish (US)
Pages (from-to)149-151
Number of pages3
JournalDiagnostic Microbiology and Infectious Disease
Volume26
Issue number3-4
DOIs
StatePublished - Nov 1 1996

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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  • Cite this

    Loeffelholz, M. J., Thompson, C. J., Gaunt, D. D., Koontz, F. P., & Gilchrist, M. J. R. (1996). Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates. Diagnostic Microbiology and Infectious Disease, 26(3-4), 149-151. https://doi.org/10.1016/S0732-8893(96)00214-3