Polymorphism of the Plasmodium falciparum multidrug resistance and chloroquine resistance transporter genes and in vitro susceptibility to aminoquinolines in isolates from the Peruvian Amazon

Maria Cecilia Huaman, Norma Roncal, Shusuke Nakazawa, Ton That Ai Long, Lucia Gerena, Coralith Garcia, Lely Solari, Alan J. Magill, Hiroji Kanbara

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

In vitro drug sensitivity to chloroquine (CQ), mefloquine (MQ) and quinine was investigated in 60 culture-adapted Plasmodium falciparum isolates from malaria patients in Padrecocha, a village in the Amazonian Department of Loreto, Peru. All isolates showed resistance to CQ, decreased susceptibility to quinine, and sensitivity to MQ. These isolates were examined for mutations in the P. falciparum multidrug resistance 1 (pfmdr1) and chloroquine resistance transporter (pfcrt) genes previously linked to CQ resistance. The mutations N86Y and D1246Y, two of the five mutations commonly observed in the pfmdr1 gene of CQ-resistant clones, were not found. The pfcrt mutation K76T, associated with CQ resistance, was identified in all the isolates tested. Sequence analysis of codons 72-76 in the pfcrt gene showed the haplotypes SVMNT and CVMNT.

Original languageEnglish (US)
Pages (from-to)461-466
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume70
Issue number5
DOIs
StatePublished - May 2004

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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