Polypurine/polypyrimidine sequences as cis-acting transcriptional regulators

Samir K. Brahmachari, Partha Sarkar, Sowmya Raghavan, Malathy Narayan, Amit K. Maiti

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Genome sequence information has generated increasing evidence for the claim that repetitive DNA sequences present within and around genes could play a important role in the regulation of gene expression. Polypurine/polypyrimidine sequences [poly(Pu/Py)] have been observed in the vicinity of promoters and within the transcribed regions of many genes. To understand whether such sequences influence the level of gene expression, we constructed several prokaryotic and eukaryotic expression vectors incorporating poly(Pu/Py) repeats both within and upstream of a reporter gene, lacZ (encoding β-galactosidase), and studied its expression in vivo. We find that, in contrast to the situation in Escherichia coli, the presence of poly(Pu/Py) sequences within the gene does not significantly inhibit gene expression in mammalian cells. On the other hand, the presence of such sequences upstream of lacZ leads to a several-fold reduction of gene expression in mammalian cells. Similar down-regulation was observed when a structural cassette containing poly(Pu/Py) sequences upstream of lacZ was integrated into yeast chromosome V. Sequence analysis of the nine totally sequenced yeast chromosomes shows that a large number of such sequences occur upstream of ORFs. On the basis of our experimental results and DNA sequence analysis, we propose that these sequences can function as cis-acting transcriptional regulators.

Original languageEnglish (US)
Pages (from-to)17-26
Number of pages10
JournalGene
Volume190
Issue number1
DOIs
StatePublished - Apr 29 1997
Externally publishedYes

Fingerprint

Gene Expression
Chromosomes
Yeasts
Galactosidases
Genes
Nucleic Acid Repetitive Sequences
Gene Expression Regulation
DNA Sequence Analysis
Reporter Genes
Open Reading Frames
Sequence Analysis
Down-Regulation
Genome
Escherichia coli

Keywords

  • Coding strand
  • DNA sequence analysis
  • Down regulation
  • Expression vectors
  • Promoter
  • Repetitive DNA
  • Triplex
  • Upstream activating sequences
  • Yeast genome

ASJC Scopus subject areas

  • Genetics

Cite this

Polypurine/polypyrimidine sequences as cis-acting transcriptional regulators. / Brahmachari, Samir K.; Sarkar, Partha; Raghavan, Sowmya; Narayan, Malathy; Maiti, Amit K.

In: Gene, Vol. 190, No. 1, 29.04.1997, p. 17-26.

Research output: Contribution to journalArticle

Brahmachari, SK, Sarkar, P, Raghavan, S, Narayan, M & Maiti, AK 1997, 'Polypurine/polypyrimidine sequences as cis-acting transcriptional regulators', Gene, vol. 190, no. 1, pp. 17-26. https://doi.org/10.1016/S0378-1119(97)00034-6
Brahmachari, Samir K. ; Sarkar, Partha ; Raghavan, Sowmya ; Narayan, Malathy ; Maiti, Amit K. / Polypurine/polypyrimidine sequences as cis-acting transcriptional regulators. In: Gene. 1997 ; Vol. 190, No. 1. pp. 17-26.
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