Polysaccharide elasticity governed by chair-boat transitions of the glucopyranose ring

Piotr E. Marszalek, Andres Oberhauser, Yuan Ping Pang, Julio M. Fernandez

Research output: Contribution to journalArticle

363 Citations (Scopus)

Abstract

Many common, biologically important polysaccharides contain pyranose rings made of five carbon atoms and one oxygen atom. They occur in a variety of cellular structures, where they are often subjected to considerable tensile stress. The polysaccharides are thought to respond to this stress by elastic deformation, but the underlying molecular rearrangements allowing such a response remain poorly understood. It is typically assumed, however, that the pyranose ring structure is inelastic and locked into a chair-like conformation. Here we describe single-molecule force measurements on individual polysaccharides that identify the pyranose rings as the structural unit controlling the molecule's elasticity. In particular, we find that the enthalpic component of the polymer elasticity of amylose, dextran and pullulan is eliminated once their pyranose rings are cleaved. We interpret these observations as indicating that the elasticity of the three polysaccharides results from a force-induced elongation of the ring structure and a final transition from a chair-like to a boat-like conformation. We expect that the force-induced deformation of pyranose rings reported here plays an important role in accommodating mechanical stresses and modulating ligand binding in biological systems.

Original languageEnglish (US)
Pages (from-to)661-664
Number of pages4
JournalNature
Volume396
Issue number6712
DOIs
StatePublished - Dec 17 1998
Externally publishedYes

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Ships
Elasticity
Polysaccharides
Amylose
Mechanical Stress
Cellular Structures
Dextrans
Polymers
Carbon
Oxygen
Ligands

ASJC Scopus subject areas

  • General

Cite this

Polysaccharide elasticity governed by chair-boat transitions of the glucopyranose ring. / Marszalek, Piotr E.; Oberhauser, Andres; Pang, Yuan Ping; Fernandez, Julio M.

In: Nature, Vol. 396, No. 6712, 17.12.1998, p. 661-664.

Research output: Contribution to journalArticle

Marszalek, Piotr E. ; Oberhauser, Andres ; Pang, Yuan Ping ; Fernandez, Julio M. / Polysaccharide elasticity governed by chair-boat transitions of the glucopyranose ring. In: Nature. 1998 ; Vol. 396, No. 6712. pp. 661-664.
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