Polysorbates prevent biofilm formation and pathogenesis of Escherichia coli O104

H4

Rudolph E. Sloup, Roberto J. Cieza, David B. Needle, Robert B. Abramovitch, Alfredo Torres, Christopher M. Waters

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Escherichia coli biotype O104:H4 recently caused the deadliest E. coli outbreak ever reported. Based on prior results, it was hypothesized that compounds inhibiting biofilm formation by O104:H4 would reduce its pathogenesis. The nonionic surfactants polysorbate 80 (PS80) and polysorbate 20 (PS20) were found to reduce biofilms by ≥ 90% at submicromolar concentrations and elicited nearly complete dispersal of preformed biofilms. PS80 did not significantly impact in vivo colonization in a mouse infection model; however, mice treated with PS80 exhibited almost no intestinal inflammation or tissue damage while untreated mice exhibited robust pathology. As PS20 and PS80 are classified as ‘Generally Recognized as Safe’ (GRAS) compounds by the Food and Drug Administration (FDA), these compounds have clinical potential to treat future O104:H4 outbreaks.

Original languageEnglish (US)
Pages (from-to)1131-1140
Number of pages10
JournalBiofouling
Volume32
Issue number9
DOIs
StatePublished - Aug 18 2016

Fingerprint

Polysorbates
Biofilms
biofilm
pathogenesis
Escherichia coli
biotype
pathology
Disease Outbreaks
surfactant
mice
colonization
nonionic surfactants
damage
United States Food and Drug Administration
biotypes
Surface-Active Agents
Escherichia coli O104
polysorbates
inflammation
Pathology

Keywords

  • Biofilm
  • Escherichia coli
  • O104:H4
  • Polysorbate

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Aquatic Science
  • Water Science and Technology

Cite this

Sloup, R. E., Cieza, R. J., Needle, D. B., Abramovitch, R. B., Torres, A., & Waters, C. M. (2016). Polysorbates prevent biofilm formation and pathogenesis of Escherichia coli O104: H4. Biofouling, 32(9), 1131-1140. https://doi.org/10.1080/08927014.2016.1230849

Polysorbates prevent biofilm formation and pathogenesis of Escherichia coli O104 : H4. / Sloup, Rudolph E.; Cieza, Roberto J.; Needle, David B.; Abramovitch, Robert B.; Torres, Alfredo; Waters, Christopher M.

In: Biofouling, Vol. 32, No. 9, 18.08.2016, p. 1131-1140.

Research output: Contribution to journalArticle

Sloup, RE, Cieza, RJ, Needle, DB, Abramovitch, RB, Torres, A & Waters, CM 2016, 'Polysorbates prevent biofilm formation and pathogenesis of Escherichia coli O104: H4', Biofouling, vol. 32, no. 9, pp. 1131-1140. https://doi.org/10.1080/08927014.2016.1230849
Sloup, Rudolph E. ; Cieza, Roberto J. ; Needle, David B. ; Abramovitch, Robert B. ; Torres, Alfredo ; Waters, Christopher M. / Polysorbates prevent biofilm formation and pathogenesis of Escherichia coli O104 : H4. In: Biofouling. 2016 ; Vol. 32, No. 9. pp. 1131-1140.
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