Possible transfer of plasmid mediated third generation cephalosporin resistance between Escherichia coli and Shigella sonnei in the human gut

Harunur Rashid, Mahbubur Rahman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Choice of antibiotic for treatment of serious bacterial infection is rapidly diminishing by plasmid mediated transfer of antibiotic resistance. Here, we report a possible horizontal transfer of plasmid carrying third-generation-cephalosporin (TGC) resistance between Escherichia coli and Shigella sonnei. Two different types of colonies were identified in MacConkey agar plate from a faecal specimen collected from a patient with shigellosis. The colonies were identified as E. coli and S. sonnei. Both of the isolates were resistant to ampicillin, chloramphenicol, co-trimoxazole, erythromycin, azithromycin, nalidixic acid, ceftriaxone, cefixime, ceftazidime, cefotaxime and susceptible to co-amoxiclave, amikacin, imipenam, astreonam, levofloxacin, moxifloxacin, mecillinam. These two strains were positive for extended spectrum β-lactamase. We were able to transfer ESBL producing property from both ceftriaxone-resistant isolates to the ceftriaxone susceptible recipient E. coli K12 and S. sonnei. Plasmid profile analysis revealed that the first-generation E. coli K12 and S. sonnei transconjugants harbored a 50. MDa R plasmid, as two-parent ESBL-producing S. sonnei and E. coli strains. Similar patterns of ESBL producing plasmid and transferable antimicrobial phenotype suggests that the ESBL producing plasmid might transferred between E. coli and S. sonnei through conjugation in the human gut.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalInfection, Genetics and Evolution
Volume30
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Fingerprint

Cephalosporin Resistance
Shigella sonnei
cephalosporins
plasmid
plasmids
Plasmids
digestive system
Escherichia coli
ceftriaxone
Ceftriaxone
Escherichia coli K12
Amdinocillin
cefixime
shigellosis
azithromycin
Cefixime
Bacillary Dysentery
amikacin
cefotaxime
Nalidixic Acid

Keywords

  • ESBL
  • Escherichia coli
  • MDR
  • R-plasmid
  • Shigella sonnei
  • Third generation cephalosporin

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Possible transfer of plasmid mediated third generation cephalosporin resistance between Escherichia coli and Shigella sonnei in the human gut. / Rashid, Harunur; Rahman, Mahbubur.

In: Infection, Genetics and Evolution, Vol. 30, 01.03.2015, p. 15-18.

Research output: Contribution to journalArticle

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