Post-ischaemic thyroid hormone treatment in a rat model of acute stroke

Tiziana Genovese, Daniela Impellizzeri, Akbar Ahmad, Carolin Cornelius, Michela Campolo, Salvatore Cuzzocrea, Emanuela Esposito

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Stroke is a devastating brain injury that is a leading cause of adult disability with limited treatment options. We examined the effects of prohormone thyroxine (T4) and the underlying mechanisms in the post-ischaemic rat brain after transient focal cerebral ischemia-induced brain injury. Ischaemic injury was induced for 2 h by middle cerebral artery occlusion (MCAo) followed by 24-h reperfusion. T4 (1.1 μg/100 g BW) was administered by intraperitoneally injection twice, at 1 after the onset of ischemia and 6 h after reperfusion. Cerebral infarct area and infarct volume were measured 24 h after MCAo. Furthermore, the mechanism of neuroprotective effect of T4 was investigated with a focus on inflammatory cells, neurotrophins, and transcriptional factors. T4 significantly reduced cerebral infarction, which were accompanied by decreased expression of proapotptic Bax and increased antiapoaptotic Bcl-2 protein. T4 suppressed the activation of astrocytes and microglia, increased the expression of neurotrophic factors (BDNF, GDNF), and altered inflammatory-related prooxidative enzymes (iNOS and COX-2) in ischaemic brain. Moreover, T4 downregulated the phosphorylation of p38 and prevented injury-induced increase of PKCδ. These results revealed that T4 has a promising therapeutic effect in ischaemic stroke treatment protecting the brain from I/R injury, probably by its anti-apoptotic, and anti-inflammatory mechanism.

Original languageEnglish (US)
Pages (from-to)92-102
Number of pages11
JournalBrain Research
Volume1513
DOIs
StatePublished - Jun 4 2013
Externally publishedYes

Fingerprint

Thyroid Hormones
Middle Cerebral Artery Infarction
Stroke
Nerve Growth Factors
Brain Injuries
Reperfusion
Wounds and Injuries
Brain
Glial Cell Line-Derived Neurotrophic Factor
Brain-Derived Neurotrophic Factor
Transient Ischemic Attack
Cerebral Infarction
Microglia
Neuroprotective Agents
Therapeutic Uses
Thyroxine
Astrocytes
Anti-Inflammatory Agents
Therapeutics
Down-Regulation

Keywords

  • Cerebral ischaemia
  • l-Thyroxine
  • Neuroprotection
  • Thyroid hormone

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Genovese, T., Impellizzeri, D., Ahmad, A., Cornelius, C., Campolo, M., Cuzzocrea, S., & Esposito, E. (2013). Post-ischaemic thyroid hormone treatment in a rat model of acute stroke. Brain Research, 1513, 92-102. https://doi.org/10.1016/j.brainres.2013.03.001

Post-ischaemic thyroid hormone treatment in a rat model of acute stroke. / Genovese, Tiziana; Impellizzeri, Daniela; Ahmad, Akbar; Cornelius, Carolin; Campolo, Michela; Cuzzocrea, Salvatore; Esposito, Emanuela.

In: Brain Research, Vol. 1513, 04.06.2013, p. 92-102.

Research output: Contribution to journalArticle

Genovese, T, Impellizzeri, D, Ahmad, A, Cornelius, C, Campolo, M, Cuzzocrea, S & Esposito, E 2013, 'Post-ischaemic thyroid hormone treatment in a rat model of acute stroke', Brain Research, vol. 1513, pp. 92-102. https://doi.org/10.1016/j.brainres.2013.03.001
Genovese T, Impellizzeri D, Ahmad A, Cornelius C, Campolo M, Cuzzocrea S et al. Post-ischaemic thyroid hormone treatment in a rat model of acute stroke. Brain Research. 2013 Jun 4;1513:92-102. https://doi.org/10.1016/j.brainres.2013.03.001
Genovese, Tiziana ; Impellizzeri, Daniela ; Ahmad, Akbar ; Cornelius, Carolin ; Campolo, Michela ; Cuzzocrea, Salvatore ; Esposito, Emanuela. / Post-ischaemic thyroid hormone treatment in a rat model of acute stroke. In: Brain Research. 2013 ; Vol. 1513. pp. 92-102.
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