Post-prandial protein handling

You are what you just ate

Bart B L Groen, Astrid M. Horstman, Henrike M. Hamer, Michiel De Haan, Janneau Van Kranenburg, Jorgen Bierau, Martijn Poeze, Will K W H Wodzig, Blake Rasmussen, Luc J C Van Loon

    Research output: Contribution to journalArticle

    24 Citations (Scopus)

    Abstract

    Background Protein turnover in skeletal muscle tissue is highly responsive to nutrient intake in healthy adults. Objective To provide a comprehensive overview of post-prandial protein handling, ranging from dietary protein digestion and amino acid absorption, the uptake of dietary protein derived amino acids over the leg, the post-prandial stimulation of muscle protein synthesis rates, to the incorporation of dietary protein derived amino acids in de novo muscle protein. Design 12 healthy young males ingested 20 g intrinsically [1-13C]-phenylalanine labeled protein. In addition, primed continuous L-[ring-2H5]-phenylalanine, L-[ring-2H2]-tyrosine, and L-[1-13C]- leucine infusions were applied, with frequent collection of arterial and venous blood samples, and muscle biopsies throughout a 5 h post-prandial period. Dietary protein digestion, amino acid absorption, splanchnic amino acid extraction, amino acid uptake over the leg, and subsequent muscle protein synthesis were measured within a single in vivo human experiment. Results 55.3±2.7% of the protein-derived phenylalanine was released in the circulation during the 5 h post-prandial period. The post-prandial rise in plasma essential amino acid availability improved leg muscle protein balance (from -291±72 to 103±66 μMmin-1100 mL leg volume- 1; P

    Original languageEnglish (US)
    Article numbere0141582
    JournalPLoS One
    Volume10
    Issue number11
    DOIs
    StatePublished - Nov 10 2015

    Fingerprint

    Meals
    Dietary Proteins
    Muscle Proteins
    muscle protein
    Amino Acids
    amino acids
    dietary protein
    Leg
    legs
    Phenylalanine
    phenylalanine
    Proteins
    proteins
    Proteolysis
    Muscle
    protein synthesis
    digestion
    uptake mechanisms
    Muscles
    Essential Amino Acids

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Groen, B. B. L., Horstman, A. M., Hamer, H. M., De Haan, M., Van Kranenburg, J., Bierau, J., ... Van Loon, L. J. C. (2015). Post-prandial protein handling: You are what you just ate. PLoS One, 10(11), [e0141582]. https://doi.org/10.1371/journal.pone.0141582

    Post-prandial protein handling : You are what you just ate. / Groen, Bart B L; Horstman, Astrid M.; Hamer, Henrike M.; De Haan, Michiel; Van Kranenburg, Janneau; Bierau, Jorgen; Poeze, Martijn; Wodzig, Will K W H; Rasmussen, Blake; Van Loon, Luc J C.

    In: PLoS One, Vol. 10, No. 11, e0141582, 10.11.2015.

    Research output: Contribution to journalArticle

    Groen, BBL, Horstman, AM, Hamer, HM, De Haan, M, Van Kranenburg, J, Bierau, J, Poeze, M, Wodzig, WKWH, Rasmussen, B & Van Loon, LJC 2015, 'Post-prandial protein handling: You are what you just ate', PLoS One, vol. 10, no. 11, e0141582. https://doi.org/10.1371/journal.pone.0141582
    Groen BBL, Horstman AM, Hamer HM, De Haan M, Van Kranenburg J, Bierau J et al. Post-prandial protein handling: You are what you just ate. PLoS One. 2015 Nov 10;10(11). e0141582. https://doi.org/10.1371/journal.pone.0141582
    Groen, Bart B L ; Horstman, Astrid M. ; Hamer, Henrike M. ; De Haan, Michiel ; Van Kranenburg, Janneau ; Bierau, Jorgen ; Poeze, Martijn ; Wodzig, Will K W H ; Rasmussen, Blake ; Van Loon, Luc J C. / Post-prandial protein handling : You are what you just ate. In: PLoS One. 2015 ; Vol. 10, No. 11.
    @article{ef2022aabbcd41e5a217114387985ef3,
    title = "Post-prandial protein handling: You are what you just ate",
    abstract = "Background Protein turnover in skeletal muscle tissue is highly responsive to nutrient intake in healthy adults. Objective To provide a comprehensive overview of post-prandial protein handling, ranging from dietary protein digestion and amino acid absorption, the uptake of dietary protein derived amino acids over the leg, the post-prandial stimulation of muscle protein synthesis rates, to the incorporation of dietary protein derived amino acids in de novo muscle protein. Design 12 healthy young males ingested 20 g intrinsically [1-13C]-phenylalanine labeled protein. In addition, primed continuous L-[ring-2H5]-phenylalanine, L-[ring-2H2]-tyrosine, and L-[1-13C]- leucine infusions were applied, with frequent collection of arterial and venous blood samples, and muscle biopsies throughout a 5 h post-prandial period. Dietary protein digestion, amino acid absorption, splanchnic amino acid extraction, amino acid uptake over the leg, and subsequent muscle protein synthesis were measured within a single in vivo human experiment. Results 55.3{\^A}±2.7{\%} of the protein-derived phenylalanine was released in the circulation during the 5 h post-prandial period. The post-prandial rise in plasma essential amino acid availability improved leg muscle protein balance (from -291{\^A}±72 to 103{\^A}±66 μMmin-1100 mL leg volume- 1; P",
    author = "Groen, {Bart B L} and Horstman, {Astrid M.} and Hamer, {Henrike M.} and {De Haan}, Michiel and {Van Kranenburg}, Janneau and Jorgen Bierau and Martijn Poeze and Wodzig, {Will K W H} and Blake Rasmussen and {Van Loon}, {Luc J C}",
    year = "2015",
    month = "11",
    day = "10",
    doi = "10.1371/journal.pone.0141582",
    language = "English (US)",
    volume = "10",
    journal = "PLoS One",
    issn = "1932-6203",
    publisher = "Public Library of Science",
    number = "11",

    }

    TY - JOUR

    T1 - Post-prandial protein handling

    T2 - You are what you just ate

    AU - Groen, Bart B L

    AU - Horstman, Astrid M.

    AU - Hamer, Henrike M.

    AU - De Haan, Michiel

    AU - Van Kranenburg, Janneau

    AU - Bierau, Jorgen

    AU - Poeze, Martijn

    AU - Wodzig, Will K W H

    AU - Rasmussen, Blake

    AU - Van Loon, Luc J C

    PY - 2015/11/10

    Y1 - 2015/11/10

    N2 - Background Protein turnover in skeletal muscle tissue is highly responsive to nutrient intake in healthy adults. Objective To provide a comprehensive overview of post-prandial protein handling, ranging from dietary protein digestion and amino acid absorption, the uptake of dietary protein derived amino acids over the leg, the post-prandial stimulation of muscle protein synthesis rates, to the incorporation of dietary protein derived amino acids in de novo muscle protein. Design 12 healthy young males ingested 20 g intrinsically [1-13C]-phenylalanine labeled protein. In addition, primed continuous L-[ring-2H5]-phenylalanine, L-[ring-2H2]-tyrosine, and L-[1-13C]- leucine infusions were applied, with frequent collection of arterial and venous blood samples, and muscle biopsies throughout a 5 h post-prandial period. Dietary protein digestion, amino acid absorption, splanchnic amino acid extraction, amino acid uptake over the leg, and subsequent muscle protein synthesis were measured within a single in vivo human experiment. Results 55.3±2.7% of the protein-derived phenylalanine was released in the circulation during the 5 h post-prandial period. The post-prandial rise in plasma essential amino acid availability improved leg muscle protein balance (from -291±72 to 103±66 μMmin-1100 mL leg volume- 1; P

    AB - Background Protein turnover in skeletal muscle tissue is highly responsive to nutrient intake in healthy adults. Objective To provide a comprehensive overview of post-prandial protein handling, ranging from dietary protein digestion and amino acid absorption, the uptake of dietary protein derived amino acids over the leg, the post-prandial stimulation of muscle protein synthesis rates, to the incorporation of dietary protein derived amino acids in de novo muscle protein. Design 12 healthy young males ingested 20 g intrinsically [1-13C]-phenylalanine labeled protein. In addition, primed continuous L-[ring-2H5]-phenylalanine, L-[ring-2H2]-tyrosine, and L-[1-13C]- leucine infusions were applied, with frequent collection of arterial and venous blood samples, and muscle biopsies throughout a 5 h post-prandial period. Dietary protein digestion, amino acid absorption, splanchnic amino acid extraction, amino acid uptake over the leg, and subsequent muscle protein synthesis were measured within a single in vivo human experiment. Results 55.3±2.7% of the protein-derived phenylalanine was released in the circulation during the 5 h post-prandial period. The post-prandial rise in plasma essential amino acid availability improved leg muscle protein balance (from -291±72 to 103±66 μMmin-1100 mL leg volume- 1; P

    UR - http://www.scopus.com/inward/record.url?scp=84953208493&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84953208493&partnerID=8YFLogxK

    U2 - 10.1371/journal.pone.0141582

    DO - 10.1371/journal.pone.0141582

    M3 - Article

    VL - 10

    JO - PLoS One

    JF - PLoS One

    SN - 1932-6203

    IS - 11

    M1 - e0141582

    ER -