Postischemic recovery of contractile function is impaired in SOD2+/- but not SOD1+/- mouse hearts

Gregory K. Asimakis, Scott Lick, Cam Patterson

    Research output: Contribution to journalArticle

    103 Scopus citations

    Abstract

    Background - Reactive oxygen species (ROS) contribute to myocardial stunning. Superoxide dismutase (SOD) is a major defense mechanism against ROS. The purpose of this study was to evaluate the contributions of cytosolic (SOD1) and mitochondrial (SOD2) isoforms to protect against myocardial stunning. Methods and Results - Isolated hearts from wild-type, heterozygous (+/-) SOD1 and SOD2 knockout mice received 30 minutes of ischemia followed by 60 minutes of reperfusion. After 60 minutes of reperfusion, the heart rate multiplied by the developed pressure (HR×DP) in the wild-type and SOD1+/- hearts recovered to 92±9 and 85±7 of preischemic baseline values, respectively (P=NS). In contrast, the HR×DP was significantly lower (63±7%) in the SOD2+/- hearts compared with the wild-type hearts. Western blot analysis and enzymatic activity of tissue lysates confirmed reduction of activities of specific SOD isoforms without compensatory increase in the other isoform in the knockout animals studied. Conclusions - Postischemic functional recovery is more sensitive to a partial deficiency of SOD2 than a partial deficiency of SOD1. Therefore, modulation of the mitochondrial SOD isoform is a critical determinant in the tolerance of the heart to oxidative stress.

    Original languageEnglish (US)
    Pages (from-to)981-986
    Number of pages6
    JournalCirculation
    Volume105
    Issue number8
    DOIs
    StatePublished - Feb 26 2002

    Keywords

    • Free radicals
    • Ischemia
    • Reperfusion
    • Stunning, myocardial

    ASJC Scopus subject areas

    • Cardiology and Cardiovascular Medicine
    • Physiology (medical)

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