Postischemic recovery of mitochondrial adenine nucleotides in the heart

Gregory K. Asimakis, Joseph B. Zwischenberger, Karen Inners-McBride, Louis A. Sordahl, Vincent Conti

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Background. Adenine nucleotides (AdNs) are lost from the mitochondrial fraction of the heart cell during ischemia. It is unknown whether this pool of AdNs can be replenished after reperfusion. The purpose of this study was to evaluate the postischemic recovery of the mitochondrial AdN pool. Methods and Results. The left anterior descending coronary artery (LAD) of the canine heart was occluded for 30 minutes followed by either no reflow, 30-minute reflow, 1-day reflow, or 7-day reflow. Systolic shortening in the LAD-supplied region was absent during occlusion but recovered to approximately 30% of preocclusion values during early reperfusion. Mitochondrial and tissue AdNs (ATP, ADP, and AMP) were determined in the LAD-supplied and left circumflex-supplied (control) regions of the heart. The AdN content (expressed as percent of control values) of mitochondria from the LAD region was 55±10% (p<0.002), 64±7% (p<0.001), 81±6% (p<0.03), and 94±8% for the no-reflow, 30-minute-reflow, 1-day-reflow, and 7-day-reflow groups, respectively. The AdN content (expressed as percent of control values) of tissue samples from the LAD region was 52±9% (p<0.002), 48±12% (p<0.02), 68±5% (p<0.002), and 70±9% for the no-reflow, 30-minute-reflow, 1-day-reflow, and 7-day-reflow groups, respectively. There was a good correlation between mitochondrial and tissue AdN (r=0.95). Using initial exchange rates, adenine nucleotide translocase activities of mitochondria from the LAD and control regions were not significantly different. State 3 respiration of LAD mitochondria was depressed (approximately 25%, p<0.05) only in the no-reflow group. Acceptor control ratios of the LAD mitochondria were not significantly different from control values in any group. Conclusions. After 30 minutes of regional ischemia, postischemic restoration of the mitochondrial AdN pool occurs between 1 and 7 days; this restoration is preceded by recovery of respiratory and adenine nucleotide translocase functions. Although the abnormally low levels of AdN persist in the mitochondrial compartment during the early reperfusion period, postischemic contractile dysfunction cannot be explained by depressed mitochondrial respiratory activity.

    Original languageEnglish (US)
    Pages (from-to)2212-2220
    Number of pages9
    JournalCirculation
    Volume85
    Issue number6
    StatePublished - Jun 1992

    Fingerprint

    Adenine Nucleotides
    Mitochondria
    ATP Translocases Mitochondrial ADP
    Reperfusion
    Ischemia
    Adenosine Monophosphate
    Adenosine Diphosphate
    Canidae
    Coronary Vessels
    Respiration
    Adenosine Triphosphate

    Keywords

    • Adenine nucleotide translocase
    • Mitochondria
    • Myocardial ischemia
    • Reperfusion

    ASJC Scopus subject areas

    • Physiology
    • Cardiology and Cardiovascular Medicine

    Cite this

    Asimakis, G. K., Zwischenberger, J. B., Inners-McBride, K., Sordahl, L. A., & Conti, V. (1992). Postischemic recovery of mitochondrial adenine nucleotides in the heart. Circulation, 85(6), 2212-2220.

    Postischemic recovery of mitochondrial adenine nucleotides in the heart. / Asimakis, Gregory K.; Zwischenberger, Joseph B.; Inners-McBride, Karen; Sordahl, Louis A.; Conti, Vincent.

    In: Circulation, Vol. 85, No. 6, 06.1992, p. 2212-2220.

    Research output: Contribution to journalArticle

    Asimakis, GK, Zwischenberger, JB, Inners-McBride, K, Sordahl, LA & Conti, V 1992, 'Postischemic recovery of mitochondrial adenine nucleotides in the heart', Circulation, vol. 85, no. 6, pp. 2212-2220.
    Asimakis GK, Zwischenberger JB, Inners-McBride K, Sordahl LA, Conti V. Postischemic recovery of mitochondrial adenine nucleotides in the heart. Circulation. 1992 Jun;85(6):2212-2220.
    Asimakis, Gregory K. ; Zwischenberger, Joseph B. ; Inners-McBride, Karen ; Sordahl, Louis A. ; Conti, Vincent. / Postischemic recovery of mitochondrial adenine nucleotides in the heart. In: Circulation. 1992 ; Vol. 85, No. 6. pp. 2212-2220.
    @article{1b641cd086e340b080e0898763d37f1d,
    title = "Postischemic recovery of mitochondrial adenine nucleotides in the heart",
    abstract = "Background. Adenine nucleotides (AdNs) are lost from the mitochondrial fraction of the heart cell during ischemia. It is unknown whether this pool of AdNs can be replenished after reperfusion. The purpose of this study was to evaluate the postischemic recovery of the mitochondrial AdN pool. Methods and Results. The left anterior descending coronary artery (LAD) of the canine heart was occluded for 30 minutes followed by either no reflow, 30-minute reflow, 1-day reflow, or 7-day reflow. Systolic shortening in the LAD-supplied region was absent during occlusion but recovered to approximately 30{\%} of preocclusion values during early reperfusion. Mitochondrial and tissue AdNs (ATP, ADP, and AMP) were determined in the LAD-supplied and left circumflex-supplied (control) regions of the heart. The AdN content (expressed as percent of control values) of mitochondria from the LAD region was 55±10{\%} (p<0.002), 64±7{\%} (p<0.001), 81±6{\%} (p<0.03), and 94±8{\%} for the no-reflow, 30-minute-reflow, 1-day-reflow, and 7-day-reflow groups, respectively. The AdN content (expressed as percent of control values) of tissue samples from the LAD region was 52±9{\%} (p<0.002), 48±12{\%} (p<0.02), 68±5{\%} (p<0.002), and 70±9{\%} for the no-reflow, 30-minute-reflow, 1-day-reflow, and 7-day-reflow groups, respectively. There was a good correlation between mitochondrial and tissue AdN (r=0.95). Using initial exchange rates, adenine nucleotide translocase activities of mitochondria from the LAD and control regions were not significantly different. State 3 respiration of LAD mitochondria was depressed (approximately 25{\%}, p<0.05) only in the no-reflow group. Acceptor control ratios of the LAD mitochondria were not significantly different from control values in any group. Conclusions. After 30 minutes of regional ischemia, postischemic restoration of the mitochondrial AdN pool occurs between 1 and 7 days; this restoration is preceded by recovery of respiratory and adenine nucleotide translocase functions. Although the abnormally low levels of AdN persist in the mitochondrial compartment during the early reperfusion period, postischemic contractile dysfunction cannot be explained by depressed mitochondrial respiratory activity.",
    keywords = "Adenine nucleotide translocase, Mitochondria, Myocardial ischemia, Reperfusion",
    author = "Asimakis, {Gregory K.} and Zwischenberger, {Joseph B.} and Karen Inners-McBride and Sordahl, {Louis A.} and Vincent Conti",
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    T1 - Postischemic recovery of mitochondrial adenine nucleotides in the heart

    AU - Asimakis, Gregory K.

    AU - Zwischenberger, Joseph B.

    AU - Inners-McBride, Karen

    AU - Sordahl, Louis A.

    AU - Conti, Vincent

    PY - 1992/6

    Y1 - 1992/6

    N2 - Background. Adenine nucleotides (AdNs) are lost from the mitochondrial fraction of the heart cell during ischemia. It is unknown whether this pool of AdNs can be replenished after reperfusion. The purpose of this study was to evaluate the postischemic recovery of the mitochondrial AdN pool. Methods and Results. The left anterior descending coronary artery (LAD) of the canine heart was occluded for 30 minutes followed by either no reflow, 30-minute reflow, 1-day reflow, or 7-day reflow. Systolic shortening in the LAD-supplied region was absent during occlusion but recovered to approximately 30% of preocclusion values during early reperfusion. Mitochondrial and tissue AdNs (ATP, ADP, and AMP) were determined in the LAD-supplied and left circumflex-supplied (control) regions of the heart. The AdN content (expressed as percent of control values) of mitochondria from the LAD region was 55±10% (p<0.002), 64±7% (p<0.001), 81±6% (p<0.03), and 94±8% for the no-reflow, 30-minute-reflow, 1-day-reflow, and 7-day-reflow groups, respectively. The AdN content (expressed as percent of control values) of tissue samples from the LAD region was 52±9% (p<0.002), 48±12% (p<0.02), 68±5% (p<0.002), and 70±9% for the no-reflow, 30-minute-reflow, 1-day-reflow, and 7-day-reflow groups, respectively. There was a good correlation between mitochondrial and tissue AdN (r=0.95). Using initial exchange rates, adenine nucleotide translocase activities of mitochondria from the LAD and control regions were not significantly different. State 3 respiration of LAD mitochondria was depressed (approximately 25%, p<0.05) only in the no-reflow group. Acceptor control ratios of the LAD mitochondria were not significantly different from control values in any group. Conclusions. After 30 minutes of regional ischemia, postischemic restoration of the mitochondrial AdN pool occurs between 1 and 7 days; this restoration is preceded by recovery of respiratory and adenine nucleotide translocase functions. Although the abnormally low levels of AdN persist in the mitochondrial compartment during the early reperfusion period, postischemic contractile dysfunction cannot be explained by depressed mitochondrial respiratory activity.

    AB - Background. Adenine nucleotides (AdNs) are lost from the mitochondrial fraction of the heart cell during ischemia. It is unknown whether this pool of AdNs can be replenished after reperfusion. The purpose of this study was to evaluate the postischemic recovery of the mitochondrial AdN pool. Methods and Results. The left anterior descending coronary artery (LAD) of the canine heart was occluded for 30 minutes followed by either no reflow, 30-minute reflow, 1-day reflow, or 7-day reflow. Systolic shortening in the LAD-supplied region was absent during occlusion but recovered to approximately 30% of preocclusion values during early reperfusion. Mitochondrial and tissue AdNs (ATP, ADP, and AMP) were determined in the LAD-supplied and left circumflex-supplied (control) regions of the heart. The AdN content (expressed as percent of control values) of mitochondria from the LAD region was 55±10% (p<0.002), 64±7% (p<0.001), 81±6% (p<0.03), and 94±8% for the no-reflow, 30-minute-reflow, 1-day-reflow, and 7-day-reflow groups, respectively. The AdN content (expressed as percent of control values) of tissue samples from the LAD region was 52±9% (p<0.002), 48±12% (p<0.02), 68±5% (p<0.002), and 70±9% for the no-reflow, 30-minute-reflow, 1-day-reflow, and 7-day-reflow groups, respectively. There was a good correlation between mitochondrial and tissue AdN (r=0.95). Using initial exchange rates, adenine nucleotide translocase activities of mitochondria from the LAD and control regions were not significantly different. State 3 respiration of LAD mitochondria was depressed (approximately 25%, p<0.05) only in the no-reflow group. Acceptor control ratios of the LAD mitochondria were not significantly different from control values in any group. Conclusions. After 30 minutes of regional ischemia, postischemic restoration of the mitochondrial AdN pool occurs between 1 and 7 days; this restoration is preceded by recovery of respiratory and adenine nucleotide translocase functions. Although the abnormally low levels of AdN persist in the mitochondrial compartment during the early reperfusion period, postischemic contractile dysfunction cannot be explained by depressed mitochondrial respiratory activity.

    KW - Adenine nucleotide translocase

    KW - Mitochondria

    KW - Myocardial ischemia

    KW - Reperfusion

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