Postmortem Distribution of the Novel Antipsychotic Drug Quetiapine

Jay Hopenwasser, Ashraf Mozayani, Terry J. Danielson, Jeremy Harbin, Harminder S. Narula, Douglas H. Posey, Paul W. Shrode, Stephen K. Wilson, Richard Li, Luis A. Sanchez

Research output: Contribution to journalArticle

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Abstract

The objective of this research was to determine the concentrations and distribution of the atypical antipsychotic drug, quetiapine, in postmortem tissues from eight Medical Examiner cases. Quetiapine was isolated from liquid specimens and tissue homogenates by extraction at an alkaline pH into 1-chlorobutane. The 1-chlorobutane was decanted, and quetiapine, plus the internal standard (prochlorperazine), was back-extracted into 0.1N sulfuric acid. The acid layer was made basic, and quetiapine, plus the internal standard, was re-extracted into 1-chlorobutane. Quantitation was by gradient, high-pressure liquid chromatography on a C-8 ODS (2.1 × 150 mm, 5 μ) column with acetonitrile/0.1M ammonium hydroxide (pH 10) mobile phase and a photodiode array detector set at 258 nm. The apparent linear range of the assay was from 0.05 to 5.0 μg/mL. At known concentrations of 0.1 and 0.5, interday accuracy (n = 5) was 103.8 and 107.2%, respectively. Interday precision (% cv) at the same concentrations was 9.8 and 9.0, respectively. In the cases where quetiapine was not considered to have contributed to the death, the postmortem concentrations in blood, liver, and bile ranged between 0.15 and 2.7 mg/L (n = 6), 1.3 and 9.5 mg/kg (n = 8), and 10 and 46 mg/L (n = 5), respectively. In the one case involving a quetiapine overdose, concentrations in blood (19.8 mg/L), liver (12.6 mg/kg), and bile (161 mg/L) exceeded the ranges of concentrations determined in specimens from the quetiapine-unrelated deaths.

Original languageEnglish (US)
Pages (from-to)264-268
Number of pages5
JournalJournal of Analytical Toxicology
Volume28
Issue number4
StatePublished - May 2004

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Liver
Antipsychotic Agents
drug
Blood
blood
High pressure liquid chromatography
Tissue
Ammonium hydroxide
Photodiodes
Acetonitrile
Sulfuric acid
sulfuric acid
hydroxide
liquid chromatography
Assays
ammonium
assay
Detectors
liquid
Acids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Hopenwasser, J., Mozayani, A., Danielson, T. J., Harbin, J., Narula, H. S., Posey, D. H., ... Sanchez, L. A. (2004). Postmortem Distribution of the Novel Antipsychotic Drug Quetiapine. Journal of Analytical Toxicology, 28(4), 264-268.

Postmortem Distribution of the Novel Antipsychotic Drug Quetiapine. / Hopenwasser, Jay; Mozayani, Ashraf; Danielson, Terry J.; Harbin, Jeremy; Narula, Harminder S.; Posey, Douglas H.; Shrode, Paul W.; Wilson, Stephen K.; Li, Richard; Sanchez, Luis A.

In: Journal of Analytical Toxicology, Vol. 28, No. 4, 05.2004, p. 264-268.

Research output: Contribution to journalArticle

Hopenwasser, J, Mozayani, A, Danielson, TJ, Harbin, J, Narula, HS, Posey, DH, Shrode, PW, Wilson, SK, Li, R & Sanchez, LA 2004, 'Postmortem Distribution of the Novel Antipsychotic Drug Quetiapine', Journal of Analytical Toxicology, vol. 28, no. 4, pp. 264-268.
Hopenwasser J, Mozayani A, Danielson TJ, Harbin J, Narula HS, Posey DH et al. Postmortem Distribution of the Novel Antipsychotic Drug Quetiapine. Journal of Analytical Toxicology. 2004 May;28(4):264-268.
Hopenwasser, Jay ; Mozayani, Ashraf ; Danielson, Terry J. ; Harbin, Jeremy ; Narula, Harminder S. ; Posey, Douglas H. ; Shrode, Paul W. ; Wilson, Stephen K. ; Li, Richard ; Sanchez, Luis A. / Postmortem Distribution of the Novel Antipsychotic Drug Quetiapine. In: Journal of Analytical Toxicology. 2004 ; Vol. 28, No. 4. pp. 264-268.
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