Posttranscriptional regulation of albumin and α-fetoprotein messenger RNA by transforming growth factor-β1 requires de novo RNA and protein synthesis

R. Daniel Beauchamp, Hong Miao Sheng, Tawfiq Alam, Courtney Townsend, John Papaconstantinou

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Transforming growth factor-β (TGFβ) has been implicated in the regulation of hepatocyte function. We have examined TGFβ1 regulation of albumin and αfetoprotein (AFP) mRNA levels in a well differentiated mouse hepatoma cell line (BWTG3). TGFβ1 reversibly decreased steady state mRNA levels of both albumin and AFP. By nuclear run-on assays, we found that TGFβ1 caused no significant change in transcription rates for albumin or AFP. Pretreatment with actinomycin-D prevented the TGFβ1-induced decrease in albumin and AFP mRNA levels. Also, if cells were treated with actinomycin-D after a 12-h exposure to TGFβ1, actinomycin-D abrogated the further decrease in albumin and AFP mRNA levels that occurred after treatment with TGFβ1 alone. Cycloheximide pretreatment blocked the TGFβ1-induced decrease in albumin and AFP mRNA levels. TGFβ1 altered neither the rate of BWTG3 cell growth nor the levels of mRNA for the growth-associated protooncogene c-myc. These data suggest that TGFβ1 has regulatory effects on specific hepatocyte functions that are independent of growth regulatory effects. The decrease in albumin and AFP mRNAs caused by TGFβ1 is posttranscriptional and dependent upon de novo RNA and protein synthesis.

Original languageEnglish (US)
Pages (from-to)1789-1796
Number of pages8
JournalMolecular Endocrinology
Volume6
Issue number11
StatePublished - 1992

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Fetal Proteins
Transforming Growth Factors
Albumins
RNA
Messenger RNA
Proteins
Dactinomycin
Hepatocytes
Growth
Cycloheximide

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Posttranscriptional regulation of albumin and α-fetoprotein messenger RNA by transforming growth factor-β1 requires de novo RNA and protein synthesis. / Beauchamp, R. Daniel; Sheng, Hong Miao; Alam, Tawfiq; Townsend, Courtney; Papaconstantinou, John.

In: Molecular Endocrinology, Vol. 6, No. 11, 1992, p. 1789-1796.

Research output: Contribution to journalArticle

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