Posttranslational modification of proteins with fatty acids in platelets

L. Muszbek, É Ráz, Michael Laposata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Direct modification of proteins by fatty acid can occur as cotranslational N-myristoylation of an N-terminal glycine residue or as posttranslational thioesterification of cysteine residue(s). Platelets provide an excellent model system for studying the posttranslational type of modification in the absence of active protein synthesis and in the absence of protein synthesis-related protein modifications with lipids. Using this model system it was shown that thioesterification of proteins with fatty acid is less specific for palmitate than it was thought earlier and that other saturated, mono- and even polyunsaturated long chain fatty acids can also participate. The chain length and the extent of unsaturation of the protein-linked fatty acid moiety can, very likely, modulate hydrophobic protein-membrane lipid and protein-protein interactions. CD9, HLA class I glycoprotein, glycoproteins Ib, IX and IV, P-selectin and α subunits of G proteins have been demonstrated unequivocally as S-fatty acid acylated platelet proteins.

Original languageEnglish (US)
Pages (from-to)359-366
Number of pages8
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume57
Issue number4-5
StatePublished - Oct 1997
Externally publishedYes

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Post Translational Protein Processing
Platelets
Fatty Acids
Blood Platelets
Proteins
Platelet Glycoprotein GPIb-IX Complex
P-Selectin
Palmitates
Membrane Lipids
Chain length
GTP-Binding Proteins
Glycine
Cysteine
Glycoproteins
Membrane Proteins
Lipids

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Posttranslational modification of proteins with fatty acids in platelets. / Muszbek, L.; Ráz, É; Laposata, Michael.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 57, No. 4-5, 10.1997, p. 359-366.

Research output: Contribution to journalArticle

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