Potent Inhibition of Junín Virus Infection by Interferon in Murine Cells

Cheng Huang, Aida G. Walker, Ashley M. Grant, Olga A. Kolokoltsova, Nadezhda E. Yun, Alexey V. Seregin, Slobodan Paessler

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The new world arenavirus Junín virus (JUNV) is the causative agent of Argentine hemorrhagic fever, a lethal human infectious disease. Adult laboratory mice are generally resistant to peripheral infection by JUNV. The mechanism underlying the mouse resistance to JUNV infection is largely unknown. We have reported that interferon receptor knockout mice succumb to JUNV infection, indicating the critical role of interferon in restricting JUNV infection in mice. Here we report that the pathogenic and vaccine strains of JUNV were highly sensitive to interferon in murine primary cells. Treatment with low concentrations of interferon abrogated viral NP protein expression in murine cells. The replication of both JUNVs was enhanced in IRF3/IRF7 deficient cells. In addition, the vaccine strain of JUNV displayed impaired growth in primary murine cells. Our data suggested a direct and potent role of host interferon response in restricting JUNV replication in mice. The defect in viral growth for vaccine JUNV might also partially explain its attenuation in mice.

Original languageEnglish (US)
Article numbere2933
JournalPLoS Neglected Tropical Diseases
Volume8
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Virus Diseases
Interferons
Viruses
New World Arenaviruses
Vaccines
Interferon Receptors
Viral Vaccines
Viral Proteins
Virus Replication
Growth
Knockout Mice
Communicable Diseases
Fever

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Medicine(all)

Cite this

Potent Inhibition of Junín Virus Infection by Interferon in Murine Cells. / Huang, Cheng; Walker, Aida G.; Grant, Ashley M.; Kolokoltsova, Olga A.; Yun, Nadezhda E.; Seregin, Alexey V.; Paessler, Slobodan.

In: PLoS Neglected Tropical Diseases, Vol. 8, No. 6, e2933, 2014.

Research output: Contribution to journalArticle

Huang, Cheng ; Walker, Aida G. ; Grant, Ashley M. ; Kolokoltsova, Olga A. ; Yun, Nadezhda E. ; Seregin, Alexey V. ; Paessler, Slobodan. / Potent Inhibition of Junín Virus Infection by Interferon in Murine Cells. In: PLoS Neglected Tropical Diseases. 2014 ; Vol. 8, No. 6.
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