Potential of ancestral sylvatic dengue-2 viruses to re-emerge

Nikos Vasilakis, Elisabeth J. Shell, Eric B. Fokam, Peter W. Mason, Kathryn A. Hanley, D. Mark Estes, Scott Weaver

Research output: Contribution to journalArticle

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Abstract

Dengue viruses (DENV) are the most important arboviral pathogens in tropical and subtropical regions throughout the world. DENV transmission includes both a sylvatic, enzootic cycle between nonhuman primates and arboreal mosquitoes of the genus Aedes, and an urban, endemic/epidemic cycle between Aedes aegypti, a mosquito with larval development in peridomestic water containers, and human reservoir hosts. All 4 serotypes of endemic DENV evolved independently from ancestral sylvatic viruses and have become both ecologically and evolutionarily distinct; this process may have involved adaptation to (i) peridomestic mosquito vectors and/or (ii) human reservoir hosts. To test the latter hypothesis, we assessed the ability of sylvatic and endemic DENV-2 strains, representing major genotypes from Southeast Asia, West Africa and the Americas, to replicate in two surrogate human model hosts: monocyte-derived, human dendritic cells (moDCs), and mice engrafted with human hepatoma cells. Although the various DENV-2 strains showed significant inter-strain variation in mean replication titers in both models, no overall difference between sylvatic and endemic strains was detected in either model. Our findings suggest that emergence of endemic DENV strains from ancestral sylvatic strains may not have required adaptation to replicate more efficiently in human reservoir hosts, implying that the potential for re-emergence of sylvatic dengue strains into the endemic cycle is high. The shared replication profiles of the American endemic and sylvatic strains suggest that American strains have maintained or regained the ancestral phenotype.

Original languageEnglish (US)
Pages (from-to)402-412
Number of pages11
JournalVirology
Volume358
Issue number2
DOIs
StatePublished - Feb 20 2007

Fingerprint

Dengue Virus
Aedes
Culicidae
Southeastern Asia
Western Africa
Dengue
Dendritic Cells
Primates
Monocytes
Hepatocellular Carcinoma
Genotype
Viruses
Phenotype
Water

Keywords

  • Dengue virus (DENV)
  • Dengue virus type 2 (DENV-2)
  • Endemic
  • Monocyte-derived dendritic cells (moDCs)
  • Re-emergence
  • Severe combined immune deficiency (SCID) mouse
  • Sylvatic

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Vasilakis, N., Shell, E. J., Fokam, E. B., Mason, P. W., Hanley, K. A., Estes, D. M., & Weaver, S. (2007). Potential of ancestral sylvatic dengue-2 viruses to re-emerge. Virology, 358(2), 402-412. https://doi.org/10.1016/j.virol.2006.08.049

Potential of ancestral sylvatic dengue-2 viruses to re-emerge. / Vasilakis, Nikos; Shell, Elisabeth J.; Fokam, Eric B.; Mason, Peter W.; Hanley, Kathryn A.; Estes, D. Mark; Weaver, Scott.

In: Virology, Vol. 358, No. 2, 20.02.2007, p. 402-412.

Research output: Contribution to journalArticle

Vasilakis, N, Shell, EJ, Fokam, EB, Mason, PW, Hanley, KA, Estes, DM & Weaver, S 2007, 'Potential of ancestral sylvatic dengue-2 viruses to re-emerge', Virology, vol. 358, no. 2, pp. 402-412. https://doi.org/10.1016/j.virol.2006.08.049
Vasilakis N, Shell EJ, Fokam EB, Mason PW, Hanley KA, Estes DM et al. Potential of ancestral sylvatic dengue-2 viruses to re-emerge. Virology. 2007 Feb 20;358(2):402-412. https://doi.org/10.1016/j.virol.2006.08.049
Vasilakis, Nikos ; Shell, Elisabeth J. ; Fokam, Eric B. ; Mason, Peter W. ; Hanley, Kathryn A. ; Estes, D. Mark ; Weaver, Scott. / Potential of ancestral sylvatic dengue-2 viruses to re-emerge. In: Virology. 2007 ; Vol. 358, No. 2. pp. 402-412.
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