Potential of selected Senegalese Aedes spp. mosquitoes (Diptera

Culicidae) to transmit Zika virus

Cheikh Tidiane Diagne, Diawo Diallo, Oumar Faye, Yamar Ba, Ousmane Faye, Alioune Gaye, Ibrahima Dia, Ousmane Faye, Scott Weaver, Amadou Alpha Sall, Mawlouth Diallo

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Background: Zika virus (ZIKV; genus Flavivirus, family Flaviviridae) is an emerging virus of medical importance maintained in a zoonotic cycle between arboreal Aedes spp. mosquitoes and nonhuman primates in African and Asian forests. Serological evidence and virus isolations have demonstrated widespread distribution of the virus in Senegal. Several mosquito species have been found naturally infected by ZIKV but little is known about their vector competence. Methods: We assessed the vector competence of Ae. aegypti from Kedougou and Dakar, Ae. unilineatus, Ae. vittatus and Ae. luteocephalus from Kedougou in Senegal for 6 ZIKV strains using experimental oral infection. Fully engorged female mosquitoes were maintained in an environmental chamber set at 27 ± 1 °C and 80 ± 5 % Relative humidity. At day 5, 10 and 15 days post infection (dpi), individual mosquito saliva, legs/wings and bodies were tested for the presence of ZIKV genome using real time RT-PCR to estimate the infection, dissemination, and transmission rates. Results: All the species tested were infected by all viral strains but only Ae. vittatus and Ae. luteocephalus were potentially capable of transmitting ZIKV after 15 dpi with 20 and 50 % of mosquitoes, respectively, delivering epidemic (HD 78788) and prototype (MR 766) ZIKV strains in saliva. Conclusion: All the species tested here were susceptible to oral infection of ZIKV but only a low proportion of Ae. vittatus and Ae. luteocephalus had the viral genome in their saliva and thus the potential to transmit the virus. Further investigations are needed on the vector competence of other species associated with ZIKV for better understanding of the ecology and epidemiology of this virus in Senegal.

Original languageEnglish (US)
Article number492
JournalBMC Infectious Diseases
Volume15
Issue number1
DOIs
StatePublished - Nov 2 2015

Fingerprint

Aedes
Culicidae
Diptera
Senegal
Viruses
Saliva
Mental Competency
Infection
Flaviviridae
Flavivirus
Infectious Disease Transmission
Viral Genome
Zoonoses
Humidity
Ecology
Primates
Real-Time Polymerase Chain Reaction
Leg
Epidemiology
Zika Virus

Keywords

  • Aedes
  • Arboviruses
  • Oral infection
  • Senegal
  • Vector competence
  • West Africa
  • Zika virus

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Potential of selected Senegalese Aedes spp. mosquitoes (Diptera : Culicidae) to transmit Zika virus. / Diagne, Cheikh Tidiane; Diallo, Diawo; Faye, Oumar; Ba, Yamar; Faye, Ousmane; Gaye, Alioune; Dia, Ibrahima; Faye, Ousmane; Weaver, Scott; Sall, Amadou Alpha; Diallo, Mawlouth.

In: BMC Infectious Diseases, Vol. 15, No. 1, 492, 02.11.2015.

Research output: Contribution to journalArticle

Diagne, CT, Diallo, D, Faye, O, Ba, Y, Faye, O, Gaye, A, Dia, I, Faye, O, Weaver, S, Sall, AA & Diallo, M 2015, 'Potential of selected Senegalese Aedes spp. mosquitoes (Diptera: Culicidae) to transmit Zika virus', BMC Infectious Diseases, vol. 15, no. 1, 492. https://doi.org/10.1186/s12879-015-1231-2
Diagne, Cheikh Tidiane ; Diallo, Diawo ; Faye, Oumar ; Ba, Yamar ; Faye, Ousmane ; Gaye, Alioune ; Dia, Ibrahima ; Faye, Ousmane ; Weaver, Scott ; Sall, Amadou Alpha ; Diallo, Mawlouth. / Potential of selected Senegalese Aedes spp. mosquitoes (Diptera : Culicidae) to transmit Zika virus. In: BMC Infectious Diseases. 2015 ; Vol. 15, No. 1.
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abstract = "Background: Zika virus (ZIKV; genus Flavivirus, family Flaviviridae) is an emerging virus of medical importance maintained in a zoonotic cycle between arboreal Aedes spp. mosquitoes and nonhuman primates in African and Asian forests. Serological evidence and virus isolations have demonstrated widespread distribution of the virus in Senegal. Several mosquito species have been found naturally infected by ZIKV but little is known about their vector competence. Methods: We assessed the vector competence of Ae. aegypti from Kedougou and Dakar, Ae. unilineatus, Ae. vittatus and Ae. luteocephalus from Kedougou in Senegal for 6 ZIKV strains using experimental oral infection. Fully engorged female mosquitoes were maintained in an environmental chamber set at 27 ± 1 °C and 80 ± 5 {\%} Relative humidity. At day 5, 10 and 15 days post infection (dpi), individual mosquito saliva, legs/wings and bodies were tested for the presence of ZIKV genome using real time RT-PCR to estimate the infection, dissemination, and transmission rates. Results: All the species tested were infected by all viral strains but only Ae. vittatus and Ae. luteocephalus were potentially capable of transmitting ZIKV after 15 dpi with 20 and 50 {\%} of mosquitoes, respectively, delivering epidemic (HD 78788) and prototype (MR 766) ZIKV strains in saliva. Conclusion: All the species tested here were susceptible to oral infection of ZIKV but only a low proportion of Ae. vittatus and Ae. luteocephalus had the viral genome in their saliva and thus the potential to transmit the virus. Further investigations are needed on the vector competence of other species associated with ZIKV for better understanding of the ecology and epidemiology of this virus in Senegal.",
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AU - Diallo, Diawo

AU - Faye, Oumar

AU - Ba, Yamar

AU - Faye, Ousmane

AU - Gaye, Alioune

AU - Dia, Ibrahima

AU - Faye, Ousmane

AU - Weaver, Scott

AU - Sall, Amadou Alpha

AU - Diallo, Mawlouth

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KW - Vector competence

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