pp60c-Src kinase mediates growth effects of the full-length precursor progastrin1-80 peptide on rat intestinal epithelial cells, in vitro

D. Brown, U. Yallampalli, A. Owlia, Pomila Singh

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Growth factor effects of precursor forms of gastrins have become evident in recent years. However, intracellular pathways that mediate growth effects of the precursor molecules are not known. In previous studies, we reported an increase in Tyr phosphorylation of pp60c-Src in intestinal epithelial cells (IEC) in response to the fully processed form of gastrin [gastrin1-17 (G17)]. We have now examined whether c-Src kinase is similarly phosphorylated and activated in response to the full-length precursor molecule, progastrin (PG)1-80, (recombinant human PG) in IEC cells. We found a significant increase in pp60c-Src kinase activity in response to both G17 and PG (0.1-1.0 nM), suggesting that growth effects of both the precursor and fully processed gastrin molecules may be mediated via similar pathways. On the other hand, pp62c-Yes was not phosphorylated or activated in response to either G17 or PG. To examine whether c-Src kinase mediates proliferative effects of PG, IEC cells were microinjected with anti-Src-IgG and 3H-thymidine (3H-Tdr) uptake of the cells measured. Control cells received nonimmune IgG. The 3H-Tdr uptake of cells stimulated with 1.0 nM PG was significantly reduced in cells microinjected with anti-c-Src-IgG; control IgG had no effect. In cells stimulated with 1.0% fetal calf serum, microinjection with c-Src-IgG had no effect on 3H-Tdr uptake. The specificity of the effect was further confirmed by blocking the inhibitory effect of anti-c-Src-IgG with antigenic Src peptide. These results suggest that activation of c-Src kinase likely represents a critical step in mediating proliferative effects of both the precursor and fully processed forms of gastrins on IEC.

Original languageEnglish (US)
Pages (from-to)201-211
Number of pages11
JournalEndocrinology
Volume144
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

src-Family Kinases
Epithelial Cells
Peptides
Immunoglobulin G
Growth
Gastrins
Src peptide
Microinjections
In Vitro Techniques
Thymidine
big gastrin
Intercellular Signaling Peptides and Proteins
Phosphorylation
Serum
CSK tyrosine-protein kinase

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

pp60c-Src kinase mediates growth effects of the full-length precursor progastrin1-80 peptide on rat intestinal epithelial cells, in vitro. / Brown, D.; Yallampalli, U.; Owlia, A.; Singh, Pomila.

In: Endocrinology, Vol. 144, No. 1, 01.01.2003, p. 201-211.

Research output: Contribution to journalArticle

@article{377eddc78ed54e44ad5c89ce931b22b0,
title = "pp60c-Src kinase mediates growth effects of the full-length precursor progastrin1-80 peptide on rat intestinal epithelial cells, in vitro",
abstract = "Growth factor effects of precursor forms of gastrins have become evident in recent years. However, intracellular pathways that mediate growth effects of the precursor molecules are not known. In previous studies, we reported an increase in Tyr phosphorylation of pp60c-Src in intestinal epithelial cells (IEC) in response to the fully processed form of gastrin [gastrin1-17 (G17)]. We have now examined whether c-Src kinase is similarly phosphorylated and activated in response to the full-length precursor molecule, progastrin (PG)1-80, (recombinant human PG) in IEC cells. We found a significant increase in pp60c-Src kinase activity in response to both G17 and PG (0.1-1.0 nM), suggesting that growth effects of both the precursor and fully processed gastrin molecules may be mediated via similar pathways. On the other hand, pp62c-Yes was not phosphorylated or activated in response to either G17 or PG. To examine whether c-Src kinase mediates proliferative effects of PG, IEC cells were microinjected with anti-Src-IgG and 3H-thymidine (3H-Tdr) uptake of the cells measured. Control cells received nonimmune IgG. The 3H-Tdr uptake of cells stimulated with 1.0 nM PG was significantly reduced in cells microinjected with anti-c-Src-IgG; control IgG had no effect. In cells stimulated with 1.0{\%} fetal calf serum, microinjection with c-Src-IgG had no effect on 3H-Tdr uptake. The specificity of the effect was further confirmed by blocking the inhibitory effect of anti-c-Src-IgG with antigenic Src peptide. These results suggest that activation of c-Src kinase likely represents a critical step in mediating proliferative effects of both the precursor and fully processed forms of gastrins on IEC.",
author = "D. Brown and U. Yallampalli and A. Owlia and Pomila Singh",
year = "2003",
month = "1",
day = "1",
doi = "10.1210/en.2002-220501",
language = "English (US)",
volume = "144",
pages = "201--211",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "1",

}

TY - JOUR

T1 - pp60c-Src kinase mediates growth effects of the full-length precursor progastrin1-80 peptide on rat intestinal epithelial cells, in vitro

AU - Brown, D.

AU - Yallampalli, U.

AU - Owlia, A.

AU - Singh, Pomila

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Growth factor effects of precursor forms of gastrins have become evident in recent years. However, intracellular pathways that mediate growth effects of the precursor molecules are not known. In previous studies, we reported an increase in Tyr phosphorylation of pp60c-Src in intestinal epithelial cells (IEC) in response to the fully processed form of gastrin [gastrin1-17 (G17)]. We have now examined whether c-Src kinase is similarly phosphorylated and activated in response to the full-length precursor molecule, progastrin (PG)1-80, (recombinant human PG) in IEC cells. We found a significant increase in pp60c-Src kinase activity in response to both G17 and PG (0.1-1.0 nM), suggesting that growth effects of both the precursor and fully processed gastrin molecules may be mediated via similar pathways. On the other hand, pp62c-Yes was not phosphorylated or activated in response to either G17 or PG. To examine whether c-Src kinase mediates proliferative effects of PG, IEC cells were microinjected with anti-Src-IgG and 3H-thymidine (3H-Tdr) uptake of the cells measured. Control cells received nonimmune IgG. The 3H-Tdr uptake of cells stimulated with 1.0 nM PG was significantly reduced in cells microinjected with anti-c-Src-IgG; control IgG had no effect. In cells stimulated with 1.0% fetal calf serum, microinjection with c-Src-IgG had no effect on 3H-Tdr uptake. The specificity of the effect was further confirmed by blocking the inhibitory effect of anti-c-Src-IgG with antigenic Src peptide. These results suggest that activation of c-Src kinase likely represents a critical step in mediating proliferative effects of both the precursor and fully processed forms of gastrins on IEC.

AB - Growth factor effects of precursor forms of gastrins have become evident in recent years. However, intracellular pathways that mediate growth effects of the precursor molecules are not known. In previous studies, we reported an increase in Tyr phosphorylation of pp60c-Src in intestinal epithelial cells (IEC) in response to the fully processed form of gastrin [gastrin1-17 (G17)]. We have now examined whether c-Src kinase is similarly phosphorylated and activated in response to the full-length precursor molecule, progastrin (PG)1-80, (recombinant human PG) in IEC cells. We found a significant increase in pp60c-Src kinase activity in response to both G17 and PG (0.1-1.0 nM), suggesting that growth effects of both the precursor and fully processed gastrin molecules may be mediated via similar pathways. On the other hand, pp62c-Yes was not phosphorylated or activated in response to either G17 or PG. To examine whether c-Src kinase mediates proliferative effects of PG, IEC cells were microinjected with anti-Src-IgG and 3H-thymidine (3H-Tdr) uptake of the cells measured. Control cells received nonimmune IgG. The 3H-Tdr uptake of cells stimulated with 1.0 nM PG was significantly reduced in cells microinjected with anti-c-Src-IgG; control IgG had no effect. In cells stimulated with 1.0% fetal calf serum, microinjection with c-Src-IgG had no effect on 3H-Tdr uptake. The specificity of the effect was further confirmed by blocking the inhibitory effect of anti-c-Src-IgG with antigenic Src peptide. These results suggest that activation of c-Src kinase likely represents a critical step in mediating proliferative effects of both the precursor and fully processed forms of gastrins on IEC.

UR - http://www.scopus.com/inward/record.url?scp=0037207466&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037207466&partnerID=8YFLogxK

U2 - 10.1210/en.2002-220501

DO - 10.1210/en.2002-220501

M3 - Article

VL - 144

SP - 201

EP - 211

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 1

ER -