PR55α, a regulatory subunit of PP2A, specifically regulates PP2A-mediated β-catenin dephosphorylation

Wen Zhang, Jun Yang, Yajuan Liu, Xi Chen, Tianxin Yu, Jianhang Jia, Chunming Liu

Research output: Contribution to journalArticle

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Abstract

A central question in Wnt signaling is the regulation of β-catenin phosphorylation and degradation. Multiple kinases, including CKIα and GSK3, are involved in β-catenin phosphorylation. Protein phosphatases such as PP2A and PP1 have been implicated in the regulation of β-catenin. However, which phosphatase dephosphorylates β-catenin in vivo and how the specificity of β-catenin dephosphorylation is regulated are not clear. In this study, we show that PP2A regulates β-catenin phosphorylation and degradation in vivo. We demonstrate that PP2A is required for Wnt/β-catenin signaling in Drosophila. Moreover, we have identified PR55α as the regulatory subunit of PP2A that controls β-catenin phosphorylation and degradation. PR55α, but not the catalytic subunit, PP2Ac, directly interacts with β-catenin. RNA interference knockdown of PR55α elevates β-catenin phosphorylation and decreases Wnt signaling, whereas overexpressing PR55α enhances Wnt signaling. Taken together, our results suggest that PR55α specifically regulates PP2A-mediated β-catenin dephosphorylation and plays an essential role in Wnt signaling.

Original languageEnglish (US)
Pages (from-to)22649-22656
Number of pages8
JournalJournal of Biological Chemistry
Volume284
Issue number34
DOIs
StatePublished - Aug 21 2009

Fingerprint

Catenins
Phosphorylation
Degradation
Phosphoprotein Phosphatases
RNA Interference
Phosphoric Monoester Hydrolases
Drosophila
Catalytic Domain
Phosphotransferases
RNA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

PR55α, a regulatory subunit of PP2A, specifically regulates PP2A-mediated β-catenin dephosphorylation. / Zhang, Wen; Yang, Jun; Liu, Yajuan; Chen, Xi; Yu, Tianxin; Jia, Jianhang; Liu, Chunming.

In: Journal of Biological Chemistry, Vol. 284, No. 34, 21.08.2009, p. 22649-22656.

Research output: Contribution to journalArticle

Zhang, Wen ; Yang, Jun ; Liu, Yajuan ; Chen, Xi ; Yu, Tianxin ; Jia, Jianhang ; Liu, Chunming. / PR55α, a regulatory subunit of PP2A, specifically regulates PP2A-mediated β-catenin dephosphorylation. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 34. pp. 22649-22656.
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AU - Jia, Jianhang

AU - Liu, Chunming

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