TY - JOUR
T1 - Precise measurement of the resolution in light microscopy using Fourier transform
AU - Vainrub, Arnold
N1 - Funding Information:
The support of the John E. Fetzer Memorial Fund (Grant No. 23-C) is acknowledged. I am grateful to Mats Gustafsson who via correspondence made clear to me the notions of resolution in imaging. I thank Vitaly Vodyanoy and Oleg Pustovyy for making the microphotographs of Richardson slide. A. Vainrub’ email is [email protected].
PY - 2008
Y1 - 2008
N2 - The resolution power of light microscope has been accurately measured (±5%) by Fourier transform of various object images and further evaluation of the highest spatial frequency in Fourier spectrum. Any unknown shape plane object with a shape feature's size smaller than the resolution to be measured was shown to provide a reliable resolution test. This simple method gives a direct measurement of the resolution power as defined by Abbe [Archiv. F. Mikroskopische Anat. 9, 413 (1873)]. The results have been justified by comparison to a standard resolution measurement by using calibrated periodic line patterns. Notably, the approach is applicable in super-resolution light microscopy (transmission, reflection, and fluorescence), where calibrated resolution targets do not occur. It was conveniently implemented by using a compact disk as a test object and free IMAGEJ imaging software.
AB - The resolution power of light microscope has been accurately measured (±5%) by Fourier transform of various object images and further evaluation of the highest spatial frequency in Fourier spectrum. Any unknown shape plane object with a shape feature's size smaller than the resolution to be measured was shown to provide a reliable resolution test. This simple method gives a direct measurement of the resolution power as defined by Abbe [Archiv. F. Mikroskopische Anat. 9, 413 (1873)]. The results have been justified by comparison to a standard resolution measurement by using calibrated periodic line patterns. Notably, the approach is applicable in super-resolution light microscopy (transmission, reflection, and fluorescence), where calibrated resolution targets do not occur. It was conveniently implemented by using a compact disk as a test object and free IMAGEJ imaging software.
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U2 - 10.1063/1.2912954
DO - 10.1063/1.2912954
M3 - Article
C2 - 18447570
AN - SCOPUS:42949127700
SN - 0034-6748
VL - 79
JO - Review of Scientific Instruments
JF - Review of Scientific Instruments
IS - 4
M1 - 046112
ER -