Precise measurement of the resolution in light microscopy using Fourier transform

Arnold Vainrub

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    The resolution power of light microscope has been accurately measured (±5%) by Fourier transform of various object images and further evaluation of the highest spatial frequency in Fourier spectrum. Any unknown shape plane object with a shape feature's size smaller than the resolution to be measured was shown to provide a reliable resolution test. This simple method gives a direct measurement of the resolution power as defined by Abbe [Archiv. F. Mikroskopische Anat. 9, 413 (1873)]. The results have been justified by comparison to a standard resolution measurement by using calibrated periodic line patterns. Notably, the approach is applicable in super-resolution light microscopy (transmission, reflection, and fluorescence), where calibrated resolution targets do not occur. It was conveniently implemented by using a compact disk as a test object and free IMAGEJ imaging software.

    Original languageEnglish (US)
    Article number046112
    JournalReview of Scientific Instruments
    Volume79
    Issue number4
    DOIs
    StatePublished - 2008

    Fingerprint

    Optical microscopy
    Fourier transforms
    microscopy
    Microscopes
    Fluorescence
    Imaging techniques
    microscopes
    computer programs
    fluorescence
    evaluation

    ASJC Scopus subject areas

    • Instrumentation
    • Physics and Astronomy (miscellaneous)

    Cite this

    Precise measurement of the resolution in light microscopy using Fourier transform. / Vainrub, Arnold.

    In: Review of Scientific Instruments, Vol. 79, No. 4, 046112, 2008.

    Research output: Contribution to journalArticle

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