Preconditioning with dobutamine in the isolated rat heart

G. K. Asimakis, V. R. Conti

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The ability of dobutamine to precondition the isolated rat heart against postischemic contractile dysfunction was assessed. Hearts were perfused with varying concentrations of dobutamine for 5 min followed by a 5 min "washout" period and 30 min of global ischemia. The hearts were reperfused for 30 min to assess postischemic function. Dobutamine improved postischemic developed pressure, +dp/dt, heart rate × developed pressure, end diastolic pressure and coronary flow in a concentration-dependent manner. The concentration of dobutamine showing the maximum protective effect was 10-6M. Propranolol administered with dobutamine significantly attenuated the protective effect. The results indicate that transient treatment with dobutamine can precondition the rat heart against ischemia/reperfusion injury. The mechanism of protection appears to involve β-adrenergic stimulation.

Original languageEnglish (US)
Pages (from-to)177-187
Number of pages11
JournalLife Sciences
Volume57
Issue number2
DOIs
StatePublished - Jun 2 1995

Keywords

  • dobutamine
  • heart
  • ischemia
  • preconditioning

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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