Preconditioning with dobutamine in the isolated rat heart

G. K. Asimakis, V. R. Conti

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The ability of dobutamine to precondition the isolated rat heart against postischemic contractile dysfunction was assessed. Hearts were perfused with varying concentrations of dobutamine for 5 min followed by a 5 min "washout" period and 30 min of global ischemia. The hearts were reperfused for 30 min to assess postischemic function. Dobutamine improved postischemic developed pressure, +dp/dt, heart rate × developed pressure, end diastolic pressure and coronary flow in a concentration-dependent manner. The concentration of dobutamine showing the maximum protective effect was 10-6M. Propranolol administered with dobutamine significantly attenuated the protective effect. The results indicate that transient treatment with dobutamine can precondition the rat heart against ischemia/reperfusion injury. The mechanism of protection appears to involve β-adrenergic stimulation.

Original languageEnglish (US)
Pages (from-to)177-187
Number of pages11
JournalLife Sciences
Volume57
Issue number2
DOIs
StatePublished - Jun 2 1995

Keywords

  • dobutamine
  • heart
  • ischemia
  • preconditioning

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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