Prediction of protein helices with a derivative of the strip-of-helix hydrophobicity algorithm

Victor Reyes, L. Phillips, R. E. Humphreys, R. A. Lew

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The strip-of-helix hydrophobicity algorithm was devised to identify protein sequences which, when coiled as α or 310 helices, had one axial, hydrophobic strip and otherwise variably hydrophilic residues. The strip-of-helix hydrophobicity algorithm also ranked such sequences according to an index, the mean hydrophobicity of amino acids in the axial strip. This algorithm well predicted T cell-presented fragments of antigenic proteins. A derivative of this algorithm (the structural helices algorithm (SHA)) was tested for the prediction of helices in crystallographically defined proteins. For the SHA, eight amino acid sequences, 2 cycles plus one amino acid in an α helix, with strip-of-helix hydrophobicity indices greater than 2.5, were selected with overlapping segments joined. These selections were terminated according to simple 'capping rules', which took into account the roles of N-terminal Asn or Pro and C-terminal Gly in the stability of helices. In analyses of 35 crystallographically defined proteins with known α and 310 helices, the predictions with the SHA overlapped (had overlap indices χ x 0.5) with 34% of known helices, touched (had overlap indices 0.5 > x > 0) or overlapped with 66% of known helices, or were neighboring (came within 6 residues) or touched or overlapped with 82% of known helices. At each level of judging the quality of prediction, the SHA was usually less sensitive (correct predictions/total number of known helices) and more efficient (correct predictions/total number of predictions) than the Chou-Fasman and Garnier-Robson methods. It was simpler in design and calculation. The chemical mechanisms underlying these algorithms appear to apply both to protein folding and to selection of T cell-presented antigenic sequences.

Original languageEnglish (US)
Pages (from-to)12854-12858
Number of pages5
JournalJournal of Biological Chemistry
Volume264
Issue number22
StatePublished - 1989
Externally publishedYes

Fingerprint

Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Derivatives
Proteins
T-cells
Amino Acids
T-Lymphocytes
Protein folding
Protein Folding
Amino Acid Sequence

ASJC Scopus subject areas

  • Biochemistry

Cite this

Prediction of protein helices with a derivative of the strip-of-helix hydrophobicity algorithm. / Reyes, Victor; Phillips, L.; Humphreys, R. E.; Lew, R. A.

In: Journal of Biological Chemistry, Vol. 264, No. 22, 1989, p. 12854-12858.

Research output: Contribution to journalArticle

Reyes, Victor ; Phillips, L. ; Humphreys, R. E. ; Lew, R. A. / Prediction of protein helices with a derivative of the strip-of-helix hydrophobicity algorithm. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 22. pp. 12854-12858.
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