Predominance of CD8+ T lymphocytes in the inflammatory lesions of mice with acute Trypanosoma cruzi infection

Jiaren Sun, R. L. Tarleton

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Using glycol methacrylate in conjunction with avidin-biotin-peroxidase complex techniques, we studied the contribution of T cell subsets to tissue inflammation during acute Trypanosoma cruzi infection. Two mouse/parasite model systems whose parasitology and pathology behaved differently were used. In C57B1/6J mice infected with the T. cruzi Brazil strain, the levels of parasitism in blood and tissue (myocardial and skeletal muscle) reached a maximum at week 6 and decreased rapidly thereafter. Inflammatory responses in tissue corresponded with the parasitism, but decreased in intensity more gradually than that of parasitism. The T lymphocytes (Thy 1.2+) were found to be the major lymphocyte population in inflammatory cardiac and skeletal muscles (64.6-81.2%) at both three and six weeks postinfection. Among T cells, CD8+ cells (47.0-58.9%) significantly outnumbered CD4+ cells (9.3- 18.6%). The number of B cells (0-1.0%) and macrophages was low. Experiments using C3H/HeSnJ mice infected with the Sylvio X10/4 clone of T. cruzi at 30 days postinfection resulted in similar findings except for a higher CD8+:CD4+ ratio. The primary finding of this study is that Thy 1.2+CD8+CD4+ T lymphocytes are the major cell population in both heart and skeletal muscle in acute murine T. cruzi infection. The predominance of CD8+ T cells coincident with the decrease in the tissue parasite burden suggests a role for CD8+ T cells in the control of T. cruzi at the level of the infected cell.

Original languageEnglish (US)
Pages (from-to)161-169
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume48
Issue number2
StatePublished - 1993
Externally publishedYes

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Trypanosoma cruzi
T-Lymphocytes
Infection
Skeletal Muscle
Myocardium
Parasites
Parasitology
CD4-CD8 Ratio
Avidin
Inbred C3H Mouse
T-Lymphocyte Subsets
Biotin
Population
Peroxidase
Brazil
B-Lymphocytes
Clone Cells
Macrophages
Lymphocytes
Pathology

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

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title = "Predominance of CD8+ T lymphocytes in the inflammatory lesions of mice with acute Trypanosoma cruzi infection",
abstract = "Using glycol methacrylate in conjunction with avidin-biotin-peroxidase complex techniques, we studied the contribution of T cell subsets to tissue inflammation during acute Trypanosoma cruzi infection. Two mouse/parasite model systems whose parasitology and pathology behaved differently were used. In C57B1/6J mice infected with the T. cruzi Brazil strain, the levels of parasitism in blood and tissue (myocardial and skeletal muscle) reached a maximum at week 6 and decreased rapidly thereafter. Inflammatory responses in tissue corresponded with the parasitism, but decreased in intensity more gradually than that of parasitism. The T lymphocytes (Thy 1.2+) were found to be the major lymphocyte population in inflammatory cardiac and skeletal muscles (64.6-81.2{\%}) at both three and six weeks postinfection. Among T cells, CD8+ cells (47.0-58.9{\%}) significantly outnumbered CD4+ cells (9.3- 18.6{\%}). The number of B cells (0-1.0{\%}) and macrophages was low. Experiments using C3H/HeSnJ mice infected with the Sylvio X10/4 clone of T. cruzi at 30 days postinfection resulted in similar findings except for a higher CD8+:CD4+ ratio. The primary finding of this study is that Thy 1.2+CD8+CD4+ T lymphocytes are the major cell population in both heart and skeletal muscle in acute murine T. cruzi infection. The predominance of CD8+ T cells coincident with the decrease in the tissue parasite burden suggests a role for CD8+ T cells in the control of T. cruzi at the level of the infected cell.",
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AU - Tarleton, R. L.

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