TY - JOUR
T1 - Predominance of CD8+ T lymphocytes in the inflammatory lesions of mice with acute Trypanosoma cruzi infection
AU - Sun, J.
AU - Tarleton, R. L.
PY - 1993
Y1 - 1993
N2 - Using glycol methacrylate in conjunction with avidin-biotin-peroxidase complex techniques, we studied the contribution of T cell subsets to tissue inflammation during acute Trypanosoma cruzi infection. Two mouse/parasite model systems whose parasitology and pathology behaved differently were used. In C57B1/6J mice infected with the T. cruzi Brazil strain, the levels of parasitism in blood and tissue (myocardial and skeletal muscle) reached a maximum at week 6 and decreased rapidly thereafter. Inflammatory responses in tissue corresponded with the parasitism, but decreased in intensity more gradually than that of parasitism. The T lymphocytes (Thy 1.2+) were found to be the major lymphocyte population in inflammatory cardiac and skeletal muscles (64.6-81.2%) at both three and six weeks postinfection. Among T cells, CD8+ cells (47.0-58.9%) significantly outnumbered CD4+ cells (9.3- 18.6%). The number of B cells (0-1.0%) and macrophages was low. Experiments using C3H/HeSnJ mice infected with the Sylvio X10/4 clone of T. cruzi at 30 days postinfection resulted in similar findings except for a higher CD8+:CD4+ ratio. The primary finding of this study is that Thy 1.2+CD8+CD4+ T lymphocytes are the major cell population in both heart and skeletal muscle in acute murine T. cruzi infection. The predominance of CD8+ T cells coincident with the decrease in the tissue parasite burden suggests a role for CD8+ T cells in the control of T. cruzi at the level of the infected cell.
AB - Using glycol methacrylate in conjunction with avidin-biotin-peroxidase complex techniques, we studied the contribution of T cell subsets to tissue inflammation during acute Trypanosoma cruzi infection. Two mouse/parasite model systems whose parasitology and pathology behaved differently were used. In C57B1/6J mice infected with the T. cruzi Brazil strain, the levels of parasitism in blood and tissue (myocardial and skeletal muscle) reached a maximum at week 6 and decreased rapidly thereafter. Inflammatory responses in tissue corresponded with the parasitism, but decreased in intensity more gradually than that of parasitism. The T lymphocytes (Thy 1.2+) were found to be the major lymphocyte population in inflammatory cardiac and skeletal muscles (64.6-81.2%) at both three and six weeks postinfection. Among T cells, CD8+ cells (47.0-58.9%) significantly outnumbered CD4+ cells (9.3- 18.6%). The number of B cells (0-1.0%) and macrophages was low. Experiments using C3H/HeSnJ mice infected with the Sylvio X10/4 clone of T. cruzi at 30 days postinfection resulted in similar findings except for a higher CD8+:CD4+ ratio. The primary finding of this study is that Thy 1.2+CD8+CD4+ T lymphocytes are the major cell population in both heart and skeletal muscle in acute murine T. cruzi infection. The predominance of CD8+ T cells coincident with the decrease in the tissue parasite burden suggests a role for CD8+ T cells in the control of T. cruzi at the level of the infected cell.
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U2 - 10.4269/ajtmh.1993.48.161
DO - 10.4269/ajtmh.1993.48.161
M3 - Article
C2 - 8447518
AN - SCOPUS:0027475712
SN - 0002-9637
VL - 48
SP - 161
EP - 169
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 2
ER -