Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon

Lucy M. Ndip, Roland N. Ndip, Seraphine N. Esemu, David Walker, Jere McBride

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374 bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes.

Original languageEnglish (US)
Pages (from-to)163-168
Number of pages6
JournalExperimental and Applied Acarology
Volume50
Issue number2
DOIs
StatePublished - 2010

Fingerprint

Ehrlichia chaffeensis
Rhipicephalus sanguineus
Cameroon
tick
Ticks
Ehrlichia canis
ticks
Dogs
dogs
DNA
gene
disulfide bonds
puppies
Disulfides
Real-Time Polymerase Chain Reaction
amplification
quantitative polymerase chain reaction
genotype
genes
Genotype

Keywords

  • Cameroon
  • Ehrlichia canis
  • Ehrlichia chaffeensis
  • Rhipicephalus sanguineus
  • Zoonosis

ASJC Scopus subject areas

  • Insect Science
  • Ecology
  • Medicine(all)

Cite this

Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon. / Ndip, Lucy M.; Ndip, Roland N.; Esemu, Seraphine N.; Walker, David; McBride, Jere.

In: Experimental and Applied Acarology, Vol. 50, No. 2, 2010, p. 163-168.

Research output: Contribution to journalArticle

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