Preemptive intrathecal ketamine injection produces a long-lasting decrease in neuropathic pain behaviors in a rat model

Allen W. Burton, Doo H. Lee, Carl Saab, Jin Chung

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Background and Objectives. Ketamine is an N-Methyl-D-Aspartate (NMDA) receptor antagonist, which has been found to effectively treat somatic and neuropathic pain. This study examines the effect (on neuropathic pain) of preemptive ketamine using different routes of administration (intrathecal versus intraperitoneal). Methods. The Institutional Animal Care and Use Committee approved the study. Thirty male Sprague-Dawley rats (250-275 g) were divided into three treatment groups [intrathecal saline/intraperitoneal saline or Control (CTL), intrathecal ketamine/intraperitoneal saline (ITK), and intrathecal saline/intraperitoneal ketamine (IPK)] prior to undergoing surgery to induce neuropathic pain by tight ligation of the left L5 and L6 spinal nerves. All drugs were given 15 minutes before nerve ligation. The ITK group received intrathecal ketamine (0.5% solution, 1 mg/kg), the IPK group received intraperitoneal ketamine (0.5 % solution, 1 mg/kg), saline was given in equal volume (approximately 0.05 mL). Mechanical allodynia, cold allodynia, and ongoing pain behaviors indicative of neuropathic pain were assessed on postoperative days 1, 3, 7, and 14 using validated methods. Results. Compared with the CTL group, the ITK group showed a state of decreased mechanical allodynia, cold allodynia, and ongoing pain as revealed by the von Frey hair, acetone, and cold plate testing, respectively. Further, this decrease was sustained for at least 2 weeks. The IPK group showed intermediate results between the CTL and ITK. Conclusions. Neuropathic pain behaviors were significantly reduced for at least 2 weeks after intrathecal ketamine was preemptively administered to animals undergoing surgery to induce neuropathic pain. The mechanism of action is thought to be prevention of spinal cord sensitization.

Original languageEnglish (US)
Pages (from-to)208-213
Number of pages6
JournalRegional Anesthesia and Pain Medicine
Volume24
Issue number3
StatePublished - May 1999
Externally publishedYes

Fingerprint

Spinal Injections
Ketamine
Neuralgia
Hyperalgesia
Ligation
Animal Care Committees
Nociceptive Pain
Pain
Spinal Nerves
Acetone
N-Methyl-D-Aspartate Receptors
Hair
Sprague Dawley Rats
Spinal Cord

Keywords

  • Intrathecal ketamine
  • Neuropathic pain behaviors

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Preemptive intrathecal ketamine injection produces a long-lasting decrease in neuropathic pain behaviors in a rat model. / Burton, Allen W.; Lee, Doo H.; Saab, Carl; Chung, Jin.

In: Regional Anesthesia and Pain Medicine, Vol. 24, No. 3, 05.1999, p. 208-213.

Research output: Contribution to journalArticle

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abstract = "Background and Objectives. Ketamine is an N-Methyl-D-Aspartate (NMDA) receptor antagonist, which has been found to effectively treat somatic and neuropathic pain. This study examines the effect (on neuropathic pain) of preemptive ketamine using different routes of administration (intrathecal versus intraperitoneal). Methods. The Institutional Animal Care and Use Committee approved the study. Thirty male Sprague-Dawley rats (250-275 g) were divided into three treatment groups [intrathecal saline/intraperitoneal saline or Control (CTL), intrathecal ketamine/intraperitoneal saline (ITK), and intrathecal saline/intraperitoneal ketamine (IPK)] prior to undergoing surgery to induce neuropathic pain by tight ligation of the left L5 and L6 spinal nerves. All drugs were given 15 minutes before nerve ligation. The ITK group received intrathecal ketamine (0.5{\%} solution, 1 mg/kg), the IPK group received intraperitoneal ketamine (0.5 {\%} solution, 1 mg/kg), saline was given in equal volume (approximately 0.05 mL). Mechanical allodynia, cold allodynia, and ongoing pain behaviors indicative of neuropathic pain were assessed on postoperative days 1, 3, 7, and 14 using validated methods. Results. Compared with the CTL group, the ITK group showed a state of decreased mechanical allodynia, cold allodynia, and ongoing pain as revealed by the von Frey hair, acetone, and cold plate testing, respectively. Further, this decrease was sustained for at least 2 weeks. The IPK group showed intermediate results between the CTL and ITK. Conclusions. Neuropathic pain behaviors were significantly reduced for at least 2 weeks after intrathecal ketamine was preemptively administered to animals undergoing surgery to induce neuropathic pain. The mechanism of action is thought to be prevention of spinal cord sensitization.",
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