Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry

Carina Sihlbom, Pia Davidsson, Carol L. Nilsson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.

Original languageEnglish (US)
Pages (from-to)2294-2301
Number of pages8
JournalJournal of Proteome Research
Volume4
Issue number6
DOIs
StatePublished - Nov 2005
Externally publishedYes

Fingerprint

Cerebrospinal fluid
Mass spectrometry
Cerebrospinal Fluid
Mass Spectrometry
Glycoproteins
Clusterin
Affinity chromatography
Isoelectric Focusing
Electrophoresis
Affinity Chromatography
Neurodegenerative Diseases
Polysaccharides
Albumins
Gels
Bos taurus structural-GP protein

Keywords

  • 2-DE
  • Albumin removal
  • CSF
  • FT-ICR MS
  • Glycoprotein
  • Prefractionation

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry. / Sihlbom, Carina; Davidsson, Pia; Nilsson, Carol L.

In: Journal of Proteome Research, Vol. 4, No. 6, 11.2005, p. 2294-2301.

Research output: Contribution to journalArticle

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