Preliminary characterization of (nucleoside-2′-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses

Eloise Mastrangelo, Karen Dalle, Michela Bollati, Mario Milani, Violaine Lantez, Xavier De Lamballeire, Nadege Brisbare, Marie Pierre Egloff, Ernest Gould, Bruno Coutard, Bruno Canard, Naomi Forrester, Martino Bolognesi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Viral methyltranferases (MTase) are involved in the third step of the mRNA-capping process, transferring a methyl group from S-adenosyl-L-methionine (SAM) to the capped mRNA. MTases are classified into two groups: (guanine-N7)-methyltransferases (N7MTases), which add a methyl group onto the N7 atom of guanine, and (nucleoside-2′-O-)-methyltransferases (2′OMTases), which add a methyl group to a ribose hydroxyl. The MTases of two flaviviruses, Meaban and Yokose viruses, have been overexpressed, purified and crystallized in complex with SAM. Characterization of the crystals together with details of preliminary X-ray diffraction data collection (at 2.8 and 2.7 Å resolution, respectively) are reported here. The sequence homology relative to Dengue virus 2′OMTase and the structural conservation of specific residues in the putative active sites suggest that both enzymes belong to the 2′OMTase subgroup.

Original languageEnglish (US)
Pages (from-to)768-770
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume62
Issue number8
DOIs
StatePublished - Aug 2006
Externally publishedYes

Fingerprint

Flavivirus
S-Adenosylmethionine
nucleosides
Guanine
Methyltransferases
Viruses
Nucleosides
methionine
Messenger RNA
Dengue Virus
Crystals
Ribose
guanines
viruses
Sequence Homology
X-Ray Diffraction
Hydroxyl Radical
crystals
Conservation
Catalytic Domain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Preliminary characterization of (nucleoside-2′-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses. / Mastrangelo, Eloise; Dalle, Karen; Bollati, Michela; Milani, Mario; Lantez, Violaine; De Lamballeire, Xavier; Brisbare, Nadege; Egloff, Marie Pierre; Gould, Ernest; Coutard, Bruno; Canard, Bruno; Forrester, Naomi; Bolognesi, Martino.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 62, No. 8, 08.2006, p. 768-770.

Research output: Contribution to journalArticle

Mastrangelo, E, Dalle, K, Bollati, M, Milani, M, Lantez, V, De Lamballeire, X, Brisbare, N, Egloff, MP, Gould, E, Coutard, B, Canard, B, Forrester, N & Bolognesi, M 2006, 'Preliminary characterization of (nucleoside-2′-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 62, no. 8, pp. 768-770. https://doi.org/10.1107/S1744309106025553
Mastrangelo, Eloise ; Dalle, Karen ; Bollati, Michela ; Milani, Mario ; Lantez, Violaine ; De Lamballeire, Xavier ; Brisbare, Nadege ; Egloff, Marie Pierre ; Gould, Ernest ; Coutard, Bruno ; Canard, Bruno ; Forrester, Naomi ; Bolognesi, Martino. / Preliminary characterization of (nucleoside-2′-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2006 ; Vol. 62, No. 8. pp. 768-770.
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