Preliminary investigation of the phage φX174 crystal structure

Peter Willingmann; S. Krishnaswamy; Robert Mckenna; Thomas J. Smith; Norman H. Olson; Michael G. Rossmann; Patricia L. Stow; Nino L. Incardona

doi:10.1016/0022-2836(90)90129-A

Preliminary investigation of the phage φX174 crystal structure

Peter Willingmann, S. Krishnaswamy, Robert Mckenna, Thomas J. Smith, Norman H. Olson, Michael G. Rossmann, Patricia L. Stow, Nino L. Incardona

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Crystals of the single-stranded DNA bacteriophage φX174 have been grown. They have a monoclinic unit cell with space group P2₁, unit cell dimensions of a = 306.0 (±0.2) A ̊, b = 361.1 (±0.2) A ̊, c = 299.7 (±0.2°) a ̊A, β = 92.91° (±0.02°) and diffract to at least 2.7 Å resolution. There are two virus particles per unit cell. Packing considerations show that the mean diameter of the virus particles is 280 Å. The virus separates into two bands in a sucrose gradient. The ratio between the absorbance at 260 nm and 280 nm is 1.45 to 1.65 for the faster and 1.15 to 1.35 for the slower bands, but both bands contain intact particles. Crystals derived from these bands are isomorphous and there is no detectable difference in their structure amplitudes.

Original language	English (US)
Pages (from-to)	345-350
Number of pages	6
Journal	Journal of Molecular Biology
Volume	212
Issue number	2
DOIs	https://doi.org/10.1016/0022-2836(90)90129-A
State	Published - Mar 20 1990
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Structural Biology
Molecular Biology

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@article{9308cd7f5cfe44438b6900dc5f898039,

title = "Preliminary investigation of the phage φX174 crystal structure",

abstract = "Crystals of the single-stranded DNA bacteriophage φX174 have been grown. They have a monoclinic unit cell with space group P21, unit cell dimensions of a = 306.0 (±0.2) A ̊, b = 361.1 (±0.2) A ̊, c = 299.7 (±0.2°) a ̊A, β = 92.91° (±0.02°) and diffract to at least 2.7 {\AA} resolution. There are two virus particles per unit cell. Packing considerations show that the mean diameter of the virus particles is 280 {\AA}. The virus separates into two bands in a sucrose gradient. The ratio between the absorbance at 260 nm and 280 nm is 1.45 to 1.65 for the faster and 1.15 to 1.35 for the slower bands, but both bands contain intact particles. Crystals derived from these bands are isomorphous and there is no detectable difference in their structure amplitudes.",

author = "Peter Willingmann and S. Krishnaswamy and Robert Mckenna and Smith, {Thomas J.} and Olson, {Norman H.} and Rossmann, {Michael G.} and Stow, {Patricia L.} and Incardona, {Nino L.}",

note = "Funding Information: staff at Cornell High Energy Synchrotron Source (CHESS) where we collected data on an earlier orthorhombic cry&al form of 4X174 obtained by crystallization in the presence of CaCf,, but found to be less suitable for subsequent studies. The work was supported by an NSF and NIH grant to M.G.R. and an NSF grant to N.L.I. P.W. is supported by fellowship Wi873/1-1 from the Deutsrhe Forschungsgemeinschaft.",

year = "1990",

month = mar,

day = "20",

doi = "10.1016/0022-2836(90)90129-A",

language = "English (US)",

volume = "212",

pages = "345--350",

journal = "Journal of Molecular Biology",

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T1 - Preliminary investigation of the phage φX174 crystal structure

AU - Willingmann, Peter

AU - Krishnaswamy, S.

AU - Mckenna, Robert

AU - Smith, Thomas J.

AU - Olson, Norman H.

AU - Rossmann, Michael G.

AU - Stow, Patricia L.

AU - Incardona, Nino L.

N1 - Funding Information: staff at Cornell High Energy Synchrotron Source (CHESS) where we collected data on an earlier orthorhombic cry&al form of 4X174 obtained by crystallization in the presence of CaCf,, but found to be less suitable for subsequent studies. The work was supported by an NSF and NIH grant to M.G.R. and an NSF grant to N.L.I. P.W. is supported by fellowship Wi873/1-1 from the Deutsrhe Forschungsgemeinschaft.

PY - 1990/3/20

Y1 - 1990/3/20

N2 - Crystals of the single-stranded DNA bacteriophage φX174 have been grown. They have a monoclinic unit cell with space group P21, unit cell dimensions of a = 306.0 (±0.2) A ̊, b = 361.1 (±0.2) A ̊, c = 299.7 (±0.2°) a ̊A, β = 92.91° (±0.02°) and diffract to at least 2.7 Å resolution. There are two virus particles per unit cell. Packing considerations show that the mean diameter of the virus particles is 280 Å. The virus separates into two bands in a sucrose gradient. The ratio between the absorbance at 260 nm and 280 nm is 1.45 to 1.65 for the faster and 1.15 to 1.35 for the slower bands, but both bands contain intact particles. Crystals derived from these bands are isomorphous and there is no detectable difference in their structure amplitudes.

AB - Crystals of the single-stranded DNA bacteriophage φX174 have been grown. They have a monoclinic unit cell with space group P21, unit cell dimensions of a = 306.0 (±0.2) A ̊, b = 361.1 (±0.2) A ̊, c = 299.7 (±0.2°) a ̊A, β = 92.91° (±0.02°) and diffract to at least 2.7 Å resolution. There are two virus particles per unit cell. Packing considerations show that the mean diameter of the virus particles is 280 Å. The virus separates into two bands in a sucrose gradient. The ratio between the absorbance at 260 nm and 280 nm is 1.45 to 1.65 for the faster and 1.15 to 1.35 for the slower bands, but both bands contain intact particles. Crystals derived from these bands are isomorphous and there is no detectable difference in their structure amplitudes.

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Preliminary investigation of the phage φX174 crystal structure

Abstract

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