Preliminary investigation of the phage φX174 crystal structure

Peter Willingmann, S. Krishnaswamy, Robert Mckenna, Thomas Smith, Norman H. Olson, Michael G. Rossmann, Patricia L. Stow, Nino L. Incardona

Research output: Contribution to journalArticle

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Abstract

Crystals of the single-stranded DNA bacteriophage φX174 have been grown. They have a monoclinic unit cell with space group P21, unit cell dimensions of a = 306.0 (±0.2) A ̊, b = 361.1 (±0.2) A ̊, c = 299.7 (±0.2°) a ̊A, β = 92.91° (±0.02°) and diffract to at least 2.7 Å resolution. There are two virus particles per unit cell. Packing considerations show that the mean diameter of the virus particles is 280 Å. The virus separates into two bands in a sucrose gradient. The ratio between the absorbance at 260 nm and 280 nm is 1.45 to 1.65 for the faster and 1.15 to 1.35 for the slower bands, but both bands contain intact particles. Crystals derived from these bands are isomorphous and there is no detectable difference in their structure amplitudes.

Original languageEnglish (US)
Pages (from-to)345-350
Number of pages6
JournalJournal of Molecular Biology
Volume212
Issue number2
DOIs
StatePublished - Mar 20 1990
Externally publishedYes

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Bacteriophages
Virion
Single-Stranded DNA
Sucrose
Viruses

ASJC Scopus subject areas

  • Virology

Cite this

Willingmann, P., Krishnaswamy, S., Mckenna, R., Smith, T., Olson, N. H., Rossmann, M. G., ... Incardona, N. L. (1990). Preliminary investigation of the phage φX174 crystal structure. Journal of Molecular Biology, 212(2), 345-350. https://doi.org/10.1016/0022-2836(90)90129-A

Preliminary investigation of the phage φX174 crystal structure. / Willingmann, Peter; Krishnaswamy, S.; Mckenna, Robert; Smith, Thomas; Olson, Norman H.; Rossmann, Michael G.; Stow, Patricia L.; Incardona, Nino L.

In: Journal of Molecular Biology, Vol. 212, No. 2, 20.03.1990, p. 345-350.

Research output: Contribution to journalArticle

Willingmann, P, Krishnaswamy, S, Mckenna, R, Smith, T, Olson, NH, Rossmann, MG, Stow, PL & Incardona, NL 1990, 'Preliminary investigation of the phage φX174 crystal structure', Journal of Molecular Biology, vol. 212, no. 2, pp. 345-350. https://doi.org/10.1016/0022-2836(90)90129-A
Willingmann P, Krishnaswamy S, Mckenna R, Smith T, Olson NH, Rossmann MG et al. Preliminary investigation of the phage φX174 crystal structure. Journal of Molecular Biology. 1990 Mar 20;212(2):345-350. https://doi.org/10.1016/0022-2836(90)90129-A
Willingmann, Peter ; Krishnaswamy, S. ; Mckenna, Robert ; Smith, Thomas ; Olson, Norman H. ; Rossmann, Michael G. ; Stow, Patricia L. ; Incardona, Nino L. / Preliminary investigation of the phage φX174 crystal structure. In: Journal of Molecular Biology. 1990 ; Vol. 212, No. 2. pp. 345-350.
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