Prenatal testosterone induces sex-specific dysfunction in endothelium-dependent relaxation pathways in adult male and female rats

Vijayakumar Chinnathambi, Chandrasekhar Yallampalli, Kunju Sathishkumar

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Prenatal testosterone (T) exposure impacts postnatal cardiovascular function, leading to increases in blood pressure with associated decreased endothelium-dependent vascular relaxation in adult females. Endothelial function in males is not known. Furthermore, which of the endothelial pathways contributes to endothelial dysfunction and if there exists sex differences are not known. The objective of this study was to characterize the relative contribution of nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF) to the impaired endothelium-dependent vasodilation in prenatal Texposed adult males and females. Offspring of pregnant rats treated with T propionate or its vehicle were examined. Telemetric blood pressure levels and endothelium-dependent vascular reactivity were assessed with wire myography. Levels of nitric oxide synthase (NOS3) and Kcnn3 and Kcnn4 channel expression were examined in mesenteric arteries. Mean arterial pressure was significantly higher in T males and females than in controls. Endothelium-dependent acetylcholine relaxation was significantly lower in both T males and females. EDHFmediated relaxation was specifically blunted in T males (Emax =48.64% ± 3.73%) compared to that in control males (Emax = 81.71% ± 3.18%); however, NO-mediated relaxation was specifically impaired in T females (Emax = 36.01% ± 4.29%) compared with that in control females (Emax = 54.56% ± 6.37%). Relaxation to sodium nitroprusside and levcromakalim were unaffected with T-treatment. NOS3 protein was decreased in T females but not in T males. Kcnn3 expression was decreased in both T males and females compared to controls. These findings suggest that prenatal T leads to an increase in blood pressure in the adult offspring, associated with blunting of endothelial cell-associated relaxation and that the effects are sex-specific: EDHF-related in males and NO-related in females.

    Original languageEnglish (US)
    Article numberArticle 97
    JournalBiology of Reproduction
    Volume89
    Issue number4
    DOIs
    StatePublished - 2013

    Fingerprint

    Endothelium
    Testosterone
    Nitric Oxide
    Blood Pressure
    Vasodilation
    Myography
    Cromakalim
    Mesenteric Arteries
    Propionates
    Vascular Endothelium
    Nitroprusside
    Nitric Oxide Synthase
    Sex Characteristics
    Acetylcholine
    Arterial Pressure
    Endothelial Cells
    Proteins

    Keywords

    • Blood pressure
    • EDHF
    • Endothelium
    • Kcnn3 channels
    • NO
    • NOS3
    • Prenatal testosterone
    • Sex-specific
    • Vascular function

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Prenatal testosterone induces sex-specific dysfunction in endothelium-dependent relaxation pathways in adult male and female rats. / Chinnathambi, Vijayakumar; Yallampalli, Chandrasekhar; Sathishkumar, Kunju.

    In: Biology of Reproduction, Vol. 89, No. 4, Article 97, 2013.

    Research output: Contribution to journalArticle

    Chinnathambi, Vijayakumar ; Yallampalli, Chandrasekhar ; Sathishkumar, Kunju. / Prenatal testosterone induces sex-specific dysfunction in endothelium-dependent relaxation pathways in adult male and female rats. In: Biology of Reproduction. 2013 ; Vol. 89, No. 4.
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    AB - Prenatal testosterone (T) exposure impacts postnatal cardiovascular function, leading to increases in blood pressure with associated decreased endothelium-dependent vascular relaxation in adult females. Endothelial function in males is not known. Furthermore, which of the endothelial pathways contributes to endothelial dysfunction and if there exists sex differences are not known. The objective of this study was to characterize the relative contribution of nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF) to the impaired endothelium-dependent vasodilation in prenatal Texposed adult males and females. Offspring of pregnant rats treated with T propionate or its vehicle were examined. Telemetric blood pressure levels and endothelium-dependent vascular reactivity were assessed with wire myography. Levels of nitric oxide synthase (NOS3) and Kcnn3 and Kcnn4 channel expression were examined in mesenteric arteries. Mean arterial pressure was significantly higher in T males and females than in controls. Endothelium-dependent acetylcholine relaxation was significantly lower in both T males and females. EDHFmediated relaxation was specifically blunted in T males (Emax =48.64% ± 3.73%) compared to that in control males (Emax = 81.71% ± 3.18%); however, NO-mediated relaxation was specifically impaired in T females (Emax = 36.01% ± 4.29%) compared with that in control females (Emax = 54.56% ± 6.37%). Relaxation to sodium nitroprusside and levcromakalim were unaffected with T-treatment. NOS3 protein was decreased in T females but not in T males. Kcnn3 expression was decreased in both T males and females compared to controls. These findings suggest that prenatal T leads to an increase in blood pressure in the adult offspring, associated with blunting of endothelial cell-associated relaxation and that the effects are sex-specific: EDHF-related in males and NO-related in females.

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