Preparation and characterization of tau oligomer strains

Urmi Sengupta, Mariana Carretero-Murillo, Rakez Kayed

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

An increasing number of studies have demonstrated the existence of multiple conformational entities of tau, as have been observed for prion protein. We have developed and optimized techniques to isolate and study oligomeric tau strains both in vitro and ex vivo. Moreover, we have modified protocols that demonstrate the seeding properties of oligomeric tau strains that are capable of propagating in vivo. These methods and protocols are explained in this chapter.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages113-146
Number of pages34
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1779
ISSN (Print)1064-3745

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Keywords

  • In vitro tau seeding
  • In vivo tau propagation
  • Tau oligomer isolation
  • Tau oligomeric strains

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sengupta, U., Carretero-Murillo, M., & Kayed, R. (2018). Preparation and characterization of tau oligomer strains. In Methods in Molecular Biology (pp. 113-146). (Methods in Molecular Biology; Vol. 1779). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7816-8_9