Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells

Afshin Samali, Jiyang Cai, Boris Zhivotovsky, Dean P. Jones, Sten Orrenius

Research output: Contribution to journalArticle

415 Citations (Scopus)

Abstract

Activation of pro-caspase-3 is a central event in the execution phase of apoptosis and appears to serve as the convergence point of different apoptotic signaling pathways. Recently, mitochondria were found to play a central role in apoptosis through release of cytochrome c and activation of caspases. Moreover, a sub-population of pro-caspase-3 has been found to be localized to this organelle. In the present study, we demonstrate that pro-caspase-3 is present in the mitochondrial fraction of Jurkat T cells in a complex with the chaperone proteins Hsp60 and Hsp10. Induction of apoptosis with staurosporine led to the activation of mitochondrial pro-caspase-3 and its dissociation from the Hsps which were released from mitochondria. The release of Hsps occurred simultaneously with the release of other mitochondrial intermembrane space proteins including cytochrome c and adenylate kinase, prior to a loss of mitochondrial transmembrane potential. In in vitro systems, recombinant Hsp60 and Hsp10 accelerated the activation of pro-caspase-3 by cytochrome c and dATP in an ATP-dependent manner, consistent with their function as chaperones. This finding suggests that the release of mitochondrial Hsps may also accelerate caspase activation in the cytoplasm of intact cells.

Original languageEnglish (US)
Pages (from-to)2040-2048
Number of pages9
JournalEMBO Journal
Volume18
Issue number8
StatePublished - Apr 15 1999
Externally publishedYes

Fingerprint

Jurkat Cells
Caspase 3
Chemical activation
Cytochromes c
Mitochondria
Apoptosis
Caspases
Chaperonin 10
Chaperonin 60
Adenylate Kinase
Staurosporine
T-cells
Organelles
Membrane Potentials
Cytoplasm
Adenosine Triphosphate
T-Lymphocytes
Population
Proteins

Keywords

  • Apoptosis caspase
  • Chaperone
  • Hsp
  • Mitochondrial transmembrane potential

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Samali, A., Cai, J., Zhivotovsky, B., Jones, D. P., & Orrenius, S. (1999). Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells. EMBO Journal, 18(8), 2040-2048.

Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells. / Samali, Afshin; Cai, Jiyang; Zhivotovsky, Boris; Jones, Dean P.; Orrenius, Sten.

In: EMBO Journal, Vol. 18, No. 8, 15.04.1999, p. 2040-2048.

Research output: Contribution to journalArticle

Samali, A, Cai, J, Zhivotovsky, B, Jones, DP & Orrenius, S 1999, 'Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells', EMBO Journal, vol. 18, no. 8, pp. 2040-2048.
Samali A, Cai J, Zhivotovsky B, Jones DP, Orrenius S. Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells. EMBO Journal. 1999 Apr 15;18(8):2040-2048.
Samali, Afshin ; Cai, Jiyang ; Zhivotovsky, Boris ; Jones, Dean P. ; Orrenius, Sten. / Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells. In: EMBO Journal. 1999 ; Vol. 18, No. 8. pp. 2040-2048.
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