Presence of insulinlike growth factor receptors and lack of insulin receptors on fetal bovine smooth muscle cells

Phillip Lee, Raymond L. Hintz, Ron G. Rosenfeld, William E. Benitz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Previous investigations have demonstrated specific receptors and associated mitogenic actions for insulin and insulinlike growth factors I and II (IGF-I and II) in postnatal bovine aortic smooth muscle. Using fetal tissue we have observed different patterns of binding and action for these peptides. Smooth muscle cells isolated from near-term fetal bovine aortae were studied in early passage. Specific receptors for both IGF-I and IGF-II were identified. Specific binding averaged 5.7%/2.5×105 cells for IGF-I, and 16.2% for IGF-II, and 0.3% for insulin. High affinity K d for both IGF receptors were nanomolar. IGF-II was fivefold less potent than IGF-I in displacing IGF-I binding. IGF-I showed no affinity for the IGF-II receptor. Insulin, at physiologic concentrations, was incapable of displacing either IGF-I or IGF-II binding. Cellular incorporation of [methyl-3H]thymidine was stimulated at the lowest dose of IGF-I tested, 0.5 ng/ml. IGF-II showed no effect up to 100 ng/ml, after which a sharp increase in incorporation was noted. Insulin had a similar effect only at concentrations >0.5 μg/ml, with a maximal response noted at 5 to 10 μg/ml. Our results indicate that fetal bovine aortic smooth muscle cells have an abundance of IGF receptors but lack specific insulin receptors. In addition, IGF-II binding levels are three times higher than for IGF-I. These results are consistent with observations in other species, in which a predominance of IGF over insulin receptors has been demonstrated in fetal tissue, and provide further evidence for a role for the IGFs in embryonic cellular metabolism.

Original languageEnglish (US)
Pages (from-to)921-926
Number of pages6
JournalIn Vitro Cellular & Developmental Biology
Volume24
Issue number9
DOIs
StatePublished - Sep 1988
Externally publishedYes

Fingerprint

Growth Factor Receptors
Insulin Receptor
Insulin-Like Growth Factor II
Insulin-Like Growth Factor I
Smooth Muscle Myocytes
Muscle
Cells
Insulin
Fetus
Tissue
IGF Type 2 Receptor
IGF Type 1 Receptor
Metabolism
Thymidine
Smooth Muscle
Aorta
Intercellular Signaling Peptides and Proteins
Peptides

Keywords

  • growth factor receptors
  • insulin
  • insulinlike growth factors
  • receptors
  • smooth muscle cells

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Medicine(all)

Cite this

Presence of insulinlike growth factor receptors and lack of insulin receptors on fetal bovine smooth muscle cells. / Lee, Phillip; Hintz, Raymond L.; Rosenfeld, Ron G.; Benitz, William E.

In: In Vitro Cellular & Developmental Biology, Vol. 24, No. 9, 09.1988, p. 921-926.

Research output: Contribution to journalArticle

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