Presence of respiratory syncytial virus genomic sequences in middle ear fluid and its relationship to expression of cytokines and cell adhesion molecules

Yoshitaka Okamoto, Kazuo Kudo, Kazuo Ishikawa, Eiko Ito, Kiyoshi Togawa, Ichiro Saito, Itaru Moro, Janak Patel, Pearay L. Ogra

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The presence of respiratory syncytial virus (RSV) and several cytokines and cell adhesion molecules in middle ear effusions and mucosal tissues was evaluated using polymerase chain reaction. RSV genomic sequences were detected in 23 (52.7%) of 44 middle ear effusions tested. The sequences were detectable at an even higher rate (82.4%) in effusions of children in whom infectious virus was detected in the nasopharynx. All samples with the RSV genome contained the mRNA for interleukin-1β and -6 and tumor necrosis factor-α. The messages for these cytokines, together with intercellular adhesion molecule-1, endothelial leukocyte adhesion molecule-1, and vascular cell adhesion molecule-1, were detected in human middle ear mucosal organ cultures infected in vitro with RSV. Our results suggest that the enhanced synthesis of proinflammatory cytokines and cell adhesion molecules in the middle ear infected with RSV may contribute to the inflammatory processes in otitis media.

Original languageEnglish (US)
Pages (from-to)1277-1281
Number of pages5
JournalJournal of Infectious Diseases
Volume168
Issue number5
StatePublished - Nov 1993

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Respiratory Syncytial Viruses
Cell Adhesion Molecules
Middle Ear
Cytokines
Otitis Media with Effusion
E-Selectin
Nasopharynx
Vascular Cell Adhesion Molecule-1
Organ Culture Techniques
Otitis Media
Intercellular Adhesion Molecule-1
Interleukin-1
Interleukin-6
Mucous Membrane
Tumor Necrosis Factor-alpha
Genome
Viruses
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Presence of respiratory syncytial virus genomic sequences in middle ear fluid and its relationship to expression of cytokines and cell adhesion molecules. / Okamoto, Yoshitaka; Kudo, Kazuo; Ishikawa, Kazuo; Ito, Eiko; Togawa, Kiyoshi; Saito, Ichiro; Moro, Itaru; Patel, Janak; Ogra, Pearay L.

In: Journal of Infectious Diseases, Vol. 168, No. 5, 11.1993, p. 1277-1281.

Research output: Contribution to journalArticle

Okamoto, Y, Kudo, K, Ishikawa, K, Ito, E, Togawa, K, Saito, I, Moro, I, Patel, J & Ogra, PL 1993, 'Presence of respiratory syncytial virus genomic sequences in middle ear fluid and its relationship to expression of cytokines and cell adhesion molecules', Journal of Infectious Diseases, vol. 168, no. 5, pp. 1277-1281.
Okamoto, Yoshitaka ; Kudo, Kazuo ; Ishikawa, Kazuo ; Ito, Eiko ; Togawa, Kiyoshi ; Saito, Ichiro ; Moro, Itaru ; Patel, Janak ; Ogra, Pearay L. / Presence of respiratory syncytial virus genomic sequences in middle ear fluid and its relationship to expression of cytokines and cell adhesion molecules. In: Journal of Infectious Diseases. 1993 ; Vol. 168, No. 5. pp. 1277-1281.
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