Preservation Injury and Acute Rejection of RatIntestinal Grafts

Protection Afforded by Pyruvate

Luca Cicalese, Vladimir Subbotin, Cristiana Rastellini, Ronald T. Stanko, Abdul S. Rao, John J. Fung

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pyruvate has been shown to prevent intestinal mucosal injury after ischemia-reperfusion. The aim of the present study was to determine whether pyruvate can (1) prevent postreperfusion mucosal injury occurring after intestinal preservation and subsequent transplantation and (2) exert a protective effect on the intestinal graft mucosa during acute rejection. Preservation mucosal injury was evaluated, after 2 hours of reperfusion, by comparing grafts transplanted in a rat syngeneic combination (ACI to ACI) after 2 hours of cold preservation using pyruvate (n = 6) or placebo (n = 6). Mucosal parameters obtained during acute rejection (allogeneic combination: ACI to Lewis) were compared between placebo-treated (n =6) and pyruvate-treated (n = 6) animals. Tissue injury was evaluated by histopathologic examination, oxygen free radical production by luminol-enhanced chemiluminescence, and degree of neutrophil infiltration by myeloperoxidase staining. After reperrusion of the preserved grafts and during acute rejection, mucosal oxygen free radical levels and the number of infiltrating neutrophils were significantly (P <0.05) increased in the untreated grafts, whereas there was a statistically significant inhibition of these parame-ters in those treated with pyruvate. Mucosal injury, seen after reperfusion of the preserved grafts, was prevented by pyruvate. The histopathologic abnormalities observed in the untreated grafts during rejection were also significantly reduced by pyruvate. Treatment with pyruvate before cold preservation of intestinal grafts, in this rat model, reduced reperfusion mucosal injury, neutrophil infiltration, and oxygen free radical production. Oxygen free radicals were produced in the mucosa of the graft during acute rejection and their production was reduced by pyruvate, which exerted a protective effect on the rejecting allograft mucosa.

Original languageEnglish (US)
Pages (from-to)549-554
Number of pages6
JournalJournal of Gastrointestinal Surgery
Volume3
Issue number5
StatePublished - Sep 1999
Externally publishedYes

Fingerprint

Graft Rejection
Pyruvic Acid
Wounds and Injuries
Transplants
Free Radicals
Reactive Oxygen Species
Neutrophil Infiltration
Reperfusion Injury
Reperfusion
Mucous Membrane
Placebos
Luminol
Intestinal Mucosa
Luminescence
Peroxidase
Allografts
Neutrophils
Transplantation
Staining and Labeling

Keywords

  • Acute rejection
  • Chemiluminescence
  • Ischemia-reperfusion
  • Organ preservation
  • Oxygen free radical
  • Pyruvate
  • Small bowel transplantation

ASJC Scopus subject areas

  • Surgery

Cite this

Cicalese, L., Subbotin, V., Rastellini, C., Stanko, R. T., Rao, A. S., & Fung, J. J. (1999). Preservation Injury and Acute Rejection of RatIntestinal Grafts: Protection Afforded by Pyruvate. Journal of Gastrointestinal Surgery, 3(5), 549-554.

Preservation Injury and Acute Rejection of RatIntestinal Grafts : Protection Afforded by Pyruvate. / Cicalese, Luca; Subbotin, Vladimir; Rastellini, Cristiana; Stanko, Ronald T.; Rao, Abdul S.; Fung, John J.

In: Journal of Gastrointestinal Surgery, Vol. 3, No. 5, 09.1999, p. 549-554.

Research output: Contribution to journalArticle

Cicalese, L, Subbotin, V, Rastellini, C, Stanko, RT, Rao, AS & Fung, JJ 1999, 'Preservation Injury and Acute Rejection of RatIntestinal Grafts: Protection Afforded by Pyruvate', Journal of Gastrointestinal Surgery, vol. 3, no. 5, pp. 549-554.
Cicalese, Luca ; Subbotin, Vladimir ; Rastellini, Cristiana ; Stanko, Ronald T. ; Rao, Abdul S. ; Fung, John J. / Preservation Injury and Acute Rejection of RatIntestinal Grafts : Protection Afforded by Pyruvate. In: Journal of Gastrointestinal Surgery. 1999 ; Vol. 3, No. 5. pp. 549-554.
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