Pressure-clamp technique for measurement of the relaxation kinetics of mechanosensitive channels

Don W. McBride, Owen P. Hamill

    Research output: Contribution to journalArticlepeer-review

    34 Scopus citations

    Abstract

    The pressure-clamp technique, used in conjunction with patch-clamp techniques, allows the application of precise pressure/suction waveforms to membrane patches and whole cells. Using step perturbations in pressure, it allows rapid relaxation measurements of the latency, turn-on, turn-off and adaptation kinetics of mechanosensitive membrane ion channels. The pressure-clamp technique also provides the ability to apply gentle and reproducible sealing protocols to establish tight seals and thereby minimize membrane-cytoskeleton disruption which can otherwise alter channel properties.

    Original languageEnglish (US)
    Pages (from-to)341-345
    Number of pages5
    JournalTrends in Neurosciences
    Volume16
    Issue number9
    DOIs
    StatePublished - Sep 1993

    ASJC Scopus subject areas

    • General Neuroscience

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