Pressure-clamp technique for measurement of the relaxation kinetics of mechanosensitive channels

Don W. McBride, Owen P. Hamill

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The pressure-clamp technique, used in conjunction with patch-clamp techniques, allows the application of precise pressure/suction waveforms to membrane patches and whole cells. Using step perturbations in pressure, it allows rapid relaxation measurements of the latency, turn-on, turn-off and adaptation kinetics of mechanosensitive membrane ion channels. The pressure-clamp technique also provides the ability to apply gentle and reproducible sealing protocols to establish tight seals and thereby minimize membrane-cytoskeleton disruption which can otherwise alter channel properties.

Original languageEnglish (US)
Pages (from-to)341-345
Number of pages5
JournalTrends in Neurosciences
Volume16
Issue number9
DOIs
StatePublished - Sep 1993

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint

Dive into the research topics of 'Pressure-clamp technique for measurement of the relaxation kinetics of mechanosensitive channels'. Together they form a unique fingerprint.

Cite this