TY - JOUR
T1 - Pressure-clamp technique for measurement of the relaxation kinetics of mechanosensitive channels
AU - McBride, Don W.
AU - Hamill, Owen P.
PY - 1993/9
Y1 - 1993/9
N2 - The pressure-clamp technique, used in conjunction with patch-clamp techniques, allows the application of precise pressure/suction waveforms to membrane patches and whole cells. Using step perturbations in pressure, it allows rapid relaxation measurements of the latency, turn-on, turn-off and adaptation kinetics of mechanosensitive membrane ion channels. The pressure-clamp technique also provides the ability to apply gentle and reproducible sealing protocols to establish tight seals and thereby minimize membrane-cytoskeleton disruption which can otherwise alter channel properties.
AB - The pressure-clamp technique, used in conjunction with patch-clamp techniques, allows the application of precise pressure/suction waveforms to membrane patches and whole cells. Using step perturbations in pressure, it allows rapid relaxation measurements of the latency, turn-on, turn-off and adaptation kinetics of mechanosensitive membrane ion channels. The pressure-clamp technique also provides the ability to apply gentle and reproducible sealing protocols to establish tight seals and thereby minimize membrane-cytoskeleton disruption which can otherwise alter channel properties.
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U2 - 10.1016/0166-2236(93)90089-5
DO - 10.1016/0166-2236(93)90089-5
M3 - Article
C2 - 7694402
AN - SCOPUS:0027295838
SN - 0166-2236
VL - 16
SP - 341
EP - 345
JO - Trends in Neurosciences
JF - Trends in Neurosciences
IS - 9
ER -