Preterm labor

CD55 in maternal blood leukocytes

Stella Nowicki, Michael G. Izban, Edyta Pawelczyk, Vincent K. Agboto, Siddarth Pratap, Gayle Olson, Bogdan Nowicki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Problem: Intrauterine inflammation is a frequent and significant factor associated with the pathogenesis of preterm labor/birth (PTL/PTB). However, it remains unclear whether the intrauterine inflammatory responses activate the maternal peripheral circulation. We explored the association between PTL/PTB and the 'activation'of the peripheral circulatory system by determining whether CD55 mRNA expression within peripheral WBCs differed between PTL and control patients not in labor. Method of study: RNA was purified from white blood cells collected frompregnant women with preterm labor (n = 45), and from pregnant (n = 30) control women. CD55 gene expression was evaluated by quantitative PCR. Results: The mean CD55 mRNA level within the PTL group (0.77 ± 0.03) was 1.48-fold higher than that observed (0.52 ± 0.02) within the control group (P < 0.0001); 71% of PTL patients and only 6.7% of control subjects expressed elevated CD55 mRNA. The receiver operating characteristics (with 95% CI) of CD55 as a marker for PTL were as follows: Sensitivity, 69% (53-82%); Specificity, 93% (78-99%); Positive Predictive Value, 94% (80-99%); and Negative Predictive Value, 67% (51-80%). In the patient population that delivered prematurely (before 37weeks), 81% expressed elevated CD55 mRNA levels with a mean of 0.78 ± 0.03 and 95% CI of 0.71-0.84. The receiver operating characteristics were as follows: Sensitivity, 73% (54-88%); Specificity, 86% (71-95%); Positive Predictive Value, 81.5% (62-94%); and Negative Predictive Value, 80% (64-91%). Conclusion: Here we report for the first time that CD55 mRNA expression was elevated in the peripheral WBCs of subjects with preterm labor compared with control gestationally-matched pregnant woman and that elevated leukocyte CD55 may be a useful predictor of subsequent PTB.

Original languageEnglish (US)
Pages (from-to)360-367
Number of pages8
JournalAmerican Journal of Reproductive Immunology
Volume61
Issue number5
DOIs
StatePublished - 2009

Fingerprint

Premature Obstetric Labor
Leukocytes
Mothers
Messenger RNA
ROC Curve
Premature Birth
Cardiovascular System
Pregnant Women
RNA
Inflammation
Gene Expression
Polymerase Chain Reaction
Control Groups
Population

Keywords

  • Infection
  • Maternal circulatory immune response
  • Preterm birth
  • Preterm labor

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Nowicki, S., Izban, M. G., Pawelczyk, E., Agboto, V. K., Pratap, S., Olson, G., & Nowicki, B. (2009). Preterm labor: CD55 in maternal blood leukocytes. American Journal of Reproductive Immunology, 61(5), 360-367. https://doi.org/10.1111/j.1600-0897.2009.00702.x

Preterm labor : CD55 in maternal blood leukocytes. / Nowicki, Stella; Izban, Michael G.; Pawelczyk, Edyta; Agboto, Vincent K.; Pratap, Siddarth; Olson, Gayle; Nowicki, Bogdan.

In: American Journal of Reproductive Immunology, Vol. 61, No. 5, 2009, p. 360-367.

Research output: Contribution to journalArticle

Nowicki, S, Izban, MG, Pawelczyk, E, Agboto, VK, Pratap, S, Olson, G & Nowicki, B 2009, 'Preterm labor: CD55 in maternal blood leukocytes', American Journal of Reproductive Immunology, vol. 61, no. 5, pp. 360-367. https://doi.org/10.1111/j.1600-0897.2009.00702.x
Nowicki, Stella ; Izban, Michael G. ; Pawelczyk, Edyta ; Agboto, Vincent K. ; Pratap, Siddarth ; Olson, Gayle ; Nowicki, Bogdan. / Preterm labor : CD55 in maternal blood leukocytes. In: American Journal of Reproductive Immunology. 2009 ; Vol. 61, No. 5. pp. 360-367.
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abstract = "Problem: Intrauterine inflammation is a frequent and significant factor associated with the pathogenesis of preterm labor/birth (PTL/PTB). However, it remains unclear whether the intrauterine inflammatory responses activate the maternal peripheral circulation. We explored the association between PTL/PTB and the 'activation'of the peripheral circulatory system by determining whether CD55 mRNA expression within peripheral WBCs differed between PTL and control patients not in labor. Method of study: RNA was purified from white blood cells collected frompregnant women with preterm labor (n = 45), and from pregnant (n = 30) control women. CD55 gene expression was evaluated by quantitative PCR. Results: The mean CD55 mRNA level within the PTL group (0.77 ± 0.03) was 1.48-fold higher than that observed (0.52 ± 0.02) within the control group (P < 0.0001); 71{\%} of PTL patients and only 6.7{\%} of control subjects expressed elevated CD55 mRNA. The receiver operating characteristics (with 95{\%} CI) of CD55 as a marker for PTL were as follows: Sensitivity, 69{\%} (53-82{\%}); Specificity, 93{\%} (78-99{\%}); Positive Predictive Value, 94{\%} (80-99{\%}); and Negative Predictive Value, 67{\%} (51-80{\%}). In the patient population that delivered prematurely (before 37weeks), 81{\%} expressed elevated CD55 mRNA levels with a mean of 0.78 ± 0.03 and 95{\%} CI of 0.71-0.84. The receiver operating characteristics were as follows: Sensitivity, 73{\%} (54-88{\%}); Specificity, 86{\%} (71-95{\%}); Positive Predictive Value, 81.5{\%} (62-94{\%}); and Negative Predictive Value, 80{\%} (64-91{\%}). Conclusion: Here we report for the first time that CD55 mRNA expression was elevated in the peripheral WBCs of subjects with preterm labor compared with control gestationally-matched pregnant woman and that elevated leukocyte CD55 may be a useful predictor of subsequent PTB.",
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AU - Nowicki, Bogdan

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N2 - Problem: Intrauterine inflammation is a frequent and significant factor associated with the pathogenesis of preterm labor/birth (PTL/PTB). However, it remains unclear whether the intrauterine inflammatory responses activate the maternal peripheral circulation. We explored the association between PTL/PTB and the 'activation'of the peripheral circulatory system by determining whether CD55 mRNA expression within peripheral WBCs differed between PTL and control patients not in labor. Method of study: RNA was purified from white blood cells collected frompregnant women with preterm labor (n = 45), and from pregnant (n = 30) control women. CD55 gene expression was evaluated by quantitative PCR. Results: The mean CD55 mRNA level within the PTL group (0.77 ± 0.03) was 1.48-fold higher than that observed (0.52 ± 0.02) within the control group (P < 0.0001); 71% of PTL patients and only 6.7% of control subjects expressed elevated CD55 mRNA. The receiver operating characteristics (with 95% CI) of CD55 as a marker for PTL were as follows: Sensitivity, 69% (53-82%); Specificity, 93% (78-99%); Positive Predictive Value, 94% (80-99%); and Negative Predictive Value, 67% (51-80%). In the patient population that delivered prematurely (before 37weeks), 81% expressed elevated CD55 mRNA levels with a mean of 0.78 ± 0.03 and 95% CI of 0.71-0.84. The receiver operating characteristics were as follows: Sensitivity, 73% (54-88%); Specificity, 86% (71-95%); Positive Predictive Value, 81.5% (62-94%); and Negative Predictive Value, 80% (64-91%). Conclusion: Here we report for the first time that CD55 mRNA expression was elevated in the peripheral WBCs of subjects with preterm labor compared with control gestationally-matched pregnant woman and that elevated leukocyte CD55 may be a useful predictor of subsequent PTB.

AB - Problem: Intrauterine inflammation is a frequent and significant factor associated with the pathogenesis of preterm labor/birth (PTL/PTB). However, it remains unclear whether the intrauterine inflammatory responses activate the maternal peripheral circulation. We explored the association between PTL/PTB and the 'activation'of the peripheral circulatory system by determining whether CD55 mRNA expression within peripheral WBCs differed between PTL and control patients not in labor. Method of study: RNA was purified from white blood cells collected frompregnant women with preterm labor (n = 45), and from pregnant (n = 30) control women. CD55 gene expression was evaluated by quantitative PCR. Results: The mean CD55 mRNA level within the PTL group (0.77 ± 0.03) was 1.48-fold higher than that observed (0.52 ± 0.02) within the control group (P < 0.0001); 71% of PTL patients and only 6.7% of control subjects expressed elevated CD55 mRNA. The receiver operating characteristics (with 95% CI) of CD55 as a marker for PTL were as follows: Sensitivity, 69% (53-82%); Specificity, 93% (78-99%); Positive Predictive Value, 94% (80-99%); and Negative Predictive Value, 67% (51-80%). In the patient population that delivered prematurely (before 37weeks), 81% expressed elevated CD55 mRNA levels with a mean of 0.78 ± 0.03 and 95% CI of 0.71-0.84. The receiver operating characteristics were as follows: Sensitivity, 73% (54-88%); Specificity, 86% (71-95%); Positive Predictive Value, 81.5% (62-94%); and Negative Predictive Value, 80% (64-91%). Conclusion: Here we report for the first time that CD55 mRNA expression was elevated in the peripheral WBCs of subjects with preterm labor compared with control gestationally-matched pregnant woman and that elevated leukocyte CD55 may be a useful predictor of subsequent PTB.

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