Pretreatment with sildenafil alleviates early lung ischemia-reperfusion injury in a rat model

Pin Keng Shih, Chih Mei Cheng, Hsien Pin Li, Meei Feng Huang, Chia Wei Chiu, Jian Xun Chen, Nai Wei Chen, Shah Hwa Chou

Research output: Contribution to journalArticle

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Abstract

Background: Lung ischemia-reperfusion (I/R) injury plays an important role in lung transplantation. Less well known is the role of sildenafil in lung I/R injury; therefore, we attempted to determine whether sildenafil could alleviate lung apoptosis and tissue injury in a rat model. Methods: Forty male Sprague-Dawley rats were randomized into four groups: saline + sham, saline + I/R, sildenafil + sham, and sildenafil + I/R groups. Three hours before the operation, each rat received normal saline or sildenafil (10 mg/kg) by lavage. The animals designed to I/R injury were subjected to 2 h of ischemia induced by occlusion of left pulmonary artery, veins, and bronchus, followed by reperfusion for 2 h. The lung tissue was harvested for the analysis of the expression of Bax, Bcl-2, p53, caspase 3, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and wet/dry (W/D) weight ratio. Results: Compared with the saline + sham group, the saline + I/R group had significant increases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α, and W/D weight ratio but a decrease in Bcl-2 (P < 0.05). Compared with the saline + I/R group, sildenafil + I/R group had significant decreases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α level, and W/D weight ratio but an increase in Bcl-2 expression (P < 0.05). Compared with the sildenafil + sham group, there were significant increases in p53 and TNF-a expression in the sildenafil + I/R group (P < 0.05). Conclusions: Pretreatment with sildenafil alleviates lung apoptosis and tissue injury in a rat model.

Original languageEnglish
JournalJournal of Surgical Research
Volume185
Issue number2
DOIs
StatePublished - Dec 2013

Fingerprint

Reperfusion Injury
Reperfusion
Lung
Ischemia
Tumor Necrosis Factor-alpha
Caspase 3
Interleukin-6
Weights and Measures
Apoptosis
Sildenafil Citrate
Lung Transplantation
Pulmonary Veins
Therapeutic Irrigation
Wounds and Injuries
Bronchi
Pulmonary Artery
Sprague Dawley Rats

Keywords

  • Apoptosis
  • Ischemia-reperfusion injury
  • Sildenafil

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

Shih, P. K., Cheng, C. M., Li, H. P., Huang, M. F., Chiu, C. W., Chen, J. X., ... Chou, S. H. (2013). Pretreatment with sildenafil alleviates early lung ischemia-reperfusion injury in a rat model. Journal of Surgical Research, 185(2). https://doi.org/10.1016/j.jss.2013.07.010

Pretreatment with sildenafil alleviates early lung ischemia-reperfusion injury in a rat model. / Shih, Pin Keng; Cheng, Chih Mei; Li, Hsien Pin; Huang, Meei Feng; Chiu, Chia Wei; Chen, Jian Xun; Chen, Nai Wei; Chou, Shah Hwa.

In: Journal of Surgical Research, Vol. 185, No. 2, 12.2013.

Research output: Contribution to journalArticle

Shih, PK, Cheng, CM, Li, HP, Huang, MF, Chiu, CW, Chen, JX, Chen, NW & Chou, SH 2013, 'Pretreatment with sildenafil alleviates early lung ischemia-reperfusion injury in a rat model', Journal of Surgical Research, vol. 185, no. 2. https://doi.org/10.1016/j.jss.2013.07.010
Shih, Pin Keng ; Cheng, Chih Mei ; Li, Hsien Pin ; Huang, Meei Feng ; Chiu, Chia Wei ; Chen, Jian Xun ; Chen, Nai Wei ; Chou, Shah Hwa. / Pretreatment with sildenafil alleviates early lung ischemia-reperfusion injury in a rat model. In: Journal of Surgical Research. 2013 ; Vol. 185, No. 2.
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abstract = "Background: Lung ischemia-reperfusion (I/R) injury plays an important role in lung transplantation. Less well known is the role of sildenafil in lung I/R injury; therefore, we attempted to determine whether sildenafil could alleviate lung apoptosis and tissue injury in a rat model. Methods: Forty male Sprague-Dawley rats were randomized into four groups: saline + sham, saline + I/R, sildenafil + sham, and sildenafil + I/R groups. Three hours before the operation, each rat received normal saline or sildenafil (10 mg/kg) by lavage. The animals designed to I/R injury were subjected to 2 h of ischemia induced by occlusion of left pulmonary artery, veins, and bronchus, followed by reperfusion for 2 h. The lung tissue was harvested for the analysis of the expression of Bax, Bcl-2, p53, caspase 3, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and wet/dry (W/D) weight ratio. Results: Compared with the saline + sham group, the saline + I/R group had significant increases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α, and W/D weight ratio but a decrease in Bcl-2 (P < 0.05). Compared with the saline + I/R group, sildenafil + I/R group had significant decreases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α level, and W/D weight ratio but an increase in Bcl-2 expression (P < 0.05). Compared with the sildenafil + sham group, there were significant increases in p53 and TNF-a expression in the sildenafil + I/R group (P < 0.05). Conclusions: Pretreatment with sildenafil alleviates lung apoptosis and tissue injury in a rat model.",
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AU - Cheng, Chih Mei

AU - Li, Hsien Pin

AU - Huang, Meei Feng

AU - Chiu, Chia Wei

AU - Chen, Jian Xun

AU - Chen, Nai Wei

AU - Chou, Shah Hwa

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N2 - Background: Lung ischemia-reperfusion (I/R) injury plays an important role in lung transplantation. Less well known is the role of sildenafil in lung I/R injury; therefore, we attempted to determine whether sildenafil could alleviate lung apoptosis and tissue injury in a rat model. Methods: Forty male Sprague-Dawley rats were randomized into four groups: saline + sham, saline + I/R, sildenafil + sham, and sildenafil + I/R groups. Three hours before the operation, each rat received normal saline or sildenafil (10 mg/kg) by lavage. The animals designed to I/R injury were subjected to 2 h of ischemia induced by occlusion of left pulmonary artery, veins, and bronchus, followed by reperfusion for 2 h. The lung tissue was harvested for the analysis of the expression of Bax, Bcl-2, p53, caspase 3, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and wet/dry (W/D) weight ratio. Results: Compared with the saline + sham group, the saline + I/R group had significant increases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α, and W/D weight ratio but a decrease in Bcl-2 (P < 0.05). Compared with the saline + I/R group, sildenafil + I/R group had significant decreases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α level, and W/D weight ratio but an increase in Bcl-2 expression (P < 0.05). Compared with the sildenafil + sham group, there were significant increases in p53 and TNF-a expression in the sildenafil + I/R group (P < 0.05). Conclusions: Pretreatment with sildenafil alleviates lung apoptosis and tissue injury in a rat model.

AB - Background: Lung ischemia-reperfusion (I/R) injury plays an important role in lung transplantation. Less well known is the role of sildenafil in lung I/R injury; therefore, we attempted to determine whether sildenafil could alleviate lung apoptosis and tissue injury in a rat model. Methods: Forty male Sprague-Dawley rats were randomized into four groups: saline + sham, saline + I/R, sildenafil + sham, and sildenafil + I/R groups. Three hours before the operation, each rat received normal saline or sildenafil (10 mg/kg) by lavage. The animals designed to I/R injury were subjected to 2 h of ischemia induced by occlusion of left pulmonary artery, veins, and bronchus, followed by reperfusion for 2 h. The lung tissue was harvested for the analysis of the expression of Bax, Bcl-2, p53, caspase 3, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and wet/dry (W/D) weight ratio. Results: Compared with the saline + sham group, the saline + I/R group had significant increases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α, and W/D weight ratio but a decrease in Bcl-2 (P < 0.05). Compared with the saline + I/R group, sildenafil + I/R group had significant decreases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α level, and W/D weight ratio but an increase in Bcl-2 expression (P < 0.05). Compared with the sildenafil + sham group, there were significant increases in p53 and TNF-a expression in the sildenafil + I/R group (P < 0.05). Conclusions: Pretreatment with sildenafil alleviates lung apoptosis and tissue injury in a rat model.

KW - Apoptosis

KW - Ischemia-reperfusion injury

KW - Sildenafil

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