TY - JOUR
T1 - Pretreatment with sildenafil alleviates early lung ischemia-reperfusion injury in a rat model
AU - Shih, Pin Keng
AU - Cheng, Chih Mei
AU - Li, Hsien Pin
AU - Huang, Meei Feng
AU - Chiu, Chia Wei
AU - Chen, Jian Xun
AU - Chen, Nai Wei
AU - Chou, Shah Hwa
PY - 2013/12
Y1 - 2013/12
N2 - Background: Lung ischemia-reperfusion (I/R) injury plays an important role in lung transplantation. Less well known is the role of sildenafil in lung I/R injury; therefore, we attempted to determine whether sildenafil could alleviate lung apoptosis and tissue injury in a rat model. Methods: Forty male Sprague-Dawley rats were randomized into four groups: saline + sham, saline + I/R, sildenafil + sham, and sildenafil + I/R groups. Three hours before the operation, each rat received normal saline or sildenafil (10 mg/kg) by lavage. The animals designed to I/R injury were subjected to 2 h of ischemia induced by occlusion of left pulmonary artery, veins, and bronchus, followed by reperfusion for 2 h. The lung tissue was harvested for the analysis of the expression of Bax, Bcl-2, p53, caspase 3, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and wet/dry (W/D) weight ratio. Results: Compared with the saline + sham group, the saline + I/R group had significant increases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α, and W/D weight ratio but a decrease in Bcl-2 (P < 0.05). Compared with the saline + I/R group, sildenafil + I/R group had significant decreases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α level, and W/D weight ratio but an increase in Bcl-2 expression (P < 0.05). Compared with the sildenafil + sham group, there were significant increases in p53 and TNF-a expression in the sildenafil + I/R group (P < 0.05). Conclusions: Pretreatment with sildenafil alleviates lung apoptosis and tissue injury in a rat model.
AB - Background: Lung ischemia-reperfusion (I/R) injury plays an important role in lung transplantation. Less well known is the role of sildenafil in lung I/R injury; therefore, we attempted to determine whether sildenafil could alleviate lung apoptosis and tissue injury in a rat model. Methods: Forty male Sprague-Dawley rats were randomized into four groups: saline + sham, saline + I/R, sildenafil + sham, and sildenafil + I/R groups. Three hours before the operation, each rat received normal saline or sildenafil (10 mg/kg) by lavage. The animals designed to I/R injury were subjected to 2 h of ischemia induced by occlusion of left pulmonary artery, veins, and bronchus, followed by reperfusion for 2 h. The lung tissue was harvested for the analysis of the expression of Bax, Bcl-2, p53, caspase 3, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and wet/dry (W/D) weight ratio. Results: Compared with the saline + sham group, the saline + I/R group had significant increases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α, and W/D weight ratio but a decrease in Bcl-2 (P < 0.05). Compared with the saline + I/R group, sildenafil + I/R group had significant decreases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α level, and W/D weight ratio but an increase in Bcl-2 expression (P < 0.05). Compared with the sildenafil + sham group, there were significant increases in p53 and TNF-a expression in the sildenafil + I/R group (P < 0.05). Conclusions: Pretreatment with sildenafil alleviates lung apoptosis and tissue injury in a rat model.
KW - Apoptosis
KW - Ischemia-reperfusion injury
KW - Sildenafil
UR - http://www.scopus.com/inward/record.url?scp=84891712451&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891712451&partnerID=8YFLogxK
U2 - 10.1016/j.jss.2013.07.010
DO - 10.1016/j.jss.2013.07.010
M3 - Article
C2 - 23953793
AN - SCOPUS:84891712451
SN - 0022-4804
VL - 185
SP - e77-e83
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 2
ER -