TY - JOUR
T1 - Prevalence of Ehrlichia canis and Hepatozoon canis in sheltered dogs in southern Aburrá Valley, Colombia
AU - Cabrera-Jaramillo, Azucena
AU - Monsalve, Santiago
AU - Arroyave, Esteban
AU - Rodas, Juan David
N1 - Publisher Copyright:
© 2022 Universidad de Antioquia. Publicado por Universidad de Antioquia, Colombia.
PY - 2022/4/1
Y1 - 2022/4/1
N2 - Background: Bacteria of the Anaplasmataceae family and canine hemoparasitic protozoans transmitted by ticks are common in Colombia due to circulation and biological adaptation of vector Rhipicephalus sanguineus sensu lato (s.l.). Objective: To detect the circulation of Ehrlichia canis and Hepatozoon canis in sheltered dogs in three municipalities in southern Aburrá Valley, province of Antioquia, Colombia. Methods: Primers were used to amplify the 16S rRNA associated with the Anaplasmataceae family, dsb for Ehrlichia sp. and 18S rRNA for Hepatozoon sp. Results: Of the 357 samples of venous blood obtained, representing all the sheltered dogs in the study zone, Ehrlichia canis DNA was detected in 2.2% of individuals, showing identity of 100% with previous sequences from the GenBank. Hepatozoon canis showed 8.7% (31/357) prevalence of infection, with 100% identity to genotypes from Japan, Brazil, and Spain. Only one sequence of H. canis exhibited a phylogenic divergence concerning H. canis previously reported in Brazil and the Old World. Conclusions: This study confirms the circulation of E. canis and H. canis in asymptomatic shelter dogs in the south-central zone of the Aburrá Valley, province of Antioquia, Colombia. The present study is the first molecular detection of H. canis in the Province of Antioquia and the third report of canine hepatozoonosis from Colombia, highlighting the importance of considering this agent in veterinary clinic.
AB - Background: Bacteria of the Anaplasmataceae family and canine hemoparasitic protozoans transmitted by ticks are common in Colombia due to circulation and biological adaptation of vector Rhipicephalus sanguineus sensu lato (s.l.). Objective: To detect the circulation of Ehrlichia canis and Hepatozoon canis in sheltered dogs in three municipalities in southern Aburrá Valley, province of Antioquia, Colombia. Methods: Primers were used to amplify the 16S rRNA associated with the Anaplasmataceae family, dsb for Ehrlichia sp. and 18S rRNA for Hepatozoon sp. Results: Of the 357 samples of venous blood obtained, representing all the sheltered dogs in the study zone, Ehrlichia canis DNA was detected in 2.2% of individuals, showing identity of 100% with previous sequences from the GenBank. Hepatozoon canis showed 8.7% (31/357) prevalence of infection, with 100% identity to genotypes from Japan, Brazil, and Spain. Only one sequence of H. canis exhibited a phylogenic divergence concerning H. canis previously reported in Brazil and the Old World. Conclusions: This study confirms the circulation of E. canis and H. canis in asymptomatic shelter dogs in the south-central zone of the Aburrá Valley, province of Antioquia, Colombia. The present study is the first molecular detection of H. canis in the Province of Antioquia and the third report of canine hepatozoonosis from Colombia, highlighting the importance of considering this agent in veterinary clinic.
KW - Colombia
KW - Ehrlichia canis
KW - Hepatozoon canis
KW - diagnosis
KW - dog
KW - infection
KW - polymerase chain reaction
KW - tickborne pathogens
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U2 - 10.17533/udea.rccp.v35n2a01
DO - 10.17533/udea.rccp.v35n2a01
M3 - Article
AN - SCOPUS:85129157427
SN - 0120-0690
VL - 35
SP - 82
EP - 92
JO - Revista Colombiana de Ciencias Pecuarias
JF - Revista Colombiana de Ciencias Pecuarias
IS - 2
ER -