Prevention of influenza virus shedding and protection from lethal H1N1 challenge using a consensus 2009 H1N1 HA and NA adenovirus vector vaccine

Frank R. Jones, Elizabeth S. Gabitzsch, Younong Xu, Joseph P. Balint, Viktoriya Borisevich, Jennifer Smith, Jeanon Smith, Bi Hung Peng, Aida Walker, Magda Salazar, Slobodan Paessler

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Vaccines against emerging pathogens such as the 2009 H1N1 pandemic virus can benefit from current technologies such as rapid genomic sequencing to construct the most biologically relevant vaccine. A novel platform (Ad5 [E1-, E2b-]) has been utilized to induce immune responses to various antigenic targets. We employed this vector platform to express hemagglutinin (HA) and neuraminidase (NA) genes from 2009 H1N1 pandemic viruses. Inserts were consensuses sequences designed from viral isolate sequences and the vaccine was rapidly constructed and produced. Vaccination induced H1N1 immune responses in mice, which afforded protection from lethal virus challenge. In ferrets, vaccination protected from disease development and significantly reduced viral titers in nasal washes. H1N1 cell mediated immunity as well as antibody induction correlated with the prevention of disease symptoms and reduction of virus replication. The Ad5 [E1-, E2b-] should be evaluated for the rapid development of effective vaccines against infectious diseases.

Original languageEnglish (US)
Pages (from-to)7020-7026
Number of pages7
JournalVaccine
Volume29
Issue number40
DOIs
StatePublished - Sep 16 2011

    Fingerprint

Keywords

  • Ad5 [E1-, E2b-]
  • Adenovirus vector
  • Cell mediated immunity
  • H1N1 vaccine
  • Hemagglutinin
  • Horizontal transmission
  • Influenza
  • Neuraminidase
  • Pandemic
  • Viral shedding

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Jones, F. R., Gabitzsch, E. S., Xu, Y., Balint, J. P., Borisevich, V., Smith, J., Smith, J., Peng, B. H., Walker, A., Salazar, M., & Paessler, S. (2011). Prevention of influenza virus shedding and protection from lethal H1N1 challenge using a consensus 2009 H1N1 HA and NA adenovirus vector vaccine. Vaccine, 29(40), 7020-7026. https://doi.org/10.1016/j.vaccine.2011.07.073