Prevention of pericyte loss by trolox in diabetic rat retina

Naseem Ansari, Weiping Zhang, Eva Fulep, Ahmad Mansour

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Prolonged hyperglycemia results in a number of diabetic complications, including retinopathy. Pericyte degeneration is one of the earliest histological changes observed in the development of diabetic retinopathy. Increased free radicals generated under hyperglycemia could damage the retina, which abounds in polyunsaturated fatty acids. In the current study, a severalfold increase in thiobarbituric acid-reactive substances was found in rat retina cultured in hyperglycemic medium, which decreased significantly when trolox, an amphipathic antioxidant, was included in the medium. To examine the contribution of oxidative stress in vivo, diabetic rats were fed trolox (0.4% in the diet) during the course of the experiments. After 5 mo of hyperglycemia, whole mounts of retinal vessels were prepared and endothelial cells (E) and pericytes (P) were counted. The ratio of E/P in the retinas obtained from normal rats, diabetic rats, and diabetic rats fed trolox were 1.74 ± 0.186, 3.78 ± 0.47, and 2.32 ± 0.24, respectively. A significant restoration of pericytes by trolox suggests the involvement of oxidative injury during pericyte loss in diabetic retinopathy.

Original languageEnglish (US)
Pages (from-to)467-475
Number of pages9
JournalJournal of Toxicology and Environmental Health - Part A
Volume54
Issue number6
StatePublished - Jul 24 1998

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Pericytes
free radical
antioxidant
Retina
Rats
vessel
fatty acid
diet
damage
Hyperglycemia
acid
Diabetic Retinopathy
experiment
Polyunsaturated fatty acids
Retinal Vessels
Oxidative stress
Thiobarbituric Acid Reactive Substances
Endothelial cells
Diabetes Complications
Nutrition

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Pollution
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Prevention of pericyte loss by trolox in diabetic rat retina. / Ansari, Naseem; Zhang, Weiping; Fulep, Eva; Mansour, Ahmad.

In: Journal of Toxicology and Environmental Health - Part A, Vol. 54, No. 6, 24.07.1998, p. 467-475.

Research output: Contribution to journalArticle

Ansari, Naseem ; Zhang, Weiping ; Fulep, Eva ; Mansour, Ahmad. / Prevention of pericyte loss by trolox in diabetic rat retina. In: Journal of Toxicology and Environmental Health - Part A. 1998 ; Vol. 54, No. 6. pp. 467-475.
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