Prevention of suppressed cell-mediated immunity in burned mice with histamine-2 receptor antagonist drugs

John F. Hansbrough, Ramon Zapata Sirvent, Edward M. Bender, Verlyn Peterson

Research output: Contribution to journalArticle

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Abstract

Thermal injury has been shown to suppress many aspects of both specific and nonspecific immune responses. We investigated the effect of two histamine H-2 antagonist drugs on cell-mediated immunity in burned mice, utilizing a method of quantitating the degree of contact sensitivity elicited to the antigen, 2,4-dinitrofluorobenzene (DNFB). Following sensitization by painting the abdomen with DNFB, animals were challenged 5 days later by painting the ears; subsequent ear swelling is a sensitive and reproducible measure of cell-mediated immunity. We have previously demonstrated that burned mice are maximally immunosuppressed 10 to 14 days following burn injury. In the present study we found that daily intraperitoneal administration of appropriate doses of the H-2 antagonists cimetidine (2 and 10 mg/kg/day) and ranitidine (2 and 10 mg/kg/day) resulted in maintenance of normal cell-mediated immunity in burned animals. Neither a lower dose of ranitidine (0.2 mg/kg/day) nor higher doses of cimetidine (20 and 50 mg/kg/day) restored immunity, and diphenhydramine, an H-1 antagonist, had no effect. There was no augmentation of contact sensitivity in unburned mice treated with cimetidine. The immunorestorative effect is probably secondary to antagonism of histamine H-2 receptors on suppressor T lymphocytes, and may reflect increased suppressor cell activity in burned mice; however, other mechanisms may be involved.

Original languageEnglish (US)
Pages (from-to)150-156
Number of pages7
JournalJournal of Surgical Research
Volume39
Issue number2
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

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Histamine Receptors
Cellular Immunity
Cimetidine
Dinitrofluorobenzene
Paintings
Ranitidine
Contact Dermatitis
Pharmaceutical Preparations
Histamine
Ear
Diphenhydramine
Wounds and Injuries
Abdomen
Immunity
Hot Temperature
Maintenance
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Surgery

Cite this

Prevention of suppressed cell-mediated immunity in burned mice with histamine-2 receptor antagonist drugs. / Hansbrough, John F.; Zapata Sirvent, Ramon; Bender, Edward M.; Peterson, Verlyn.

In: Journal of Surgical Research, Vol. 39, No. 2, 01.01.1985, p. 150-156.

Research output: Contribution to journalArticle

Hansbrough, John F. ; Zapata Sirvent, Ramon ; Bender, Edward M. ; Peterson, Verlyn. / Prevention of suppressed cell-mediated immunity in burned mice with histamine-2 receptor antagonist drugs. In: Journal of Surgical Research. 1985 ; Vol. 39, No. 2. pp. 150-156.
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