Primary and anamnestic responses of bovine bronchoalveolar and peripheral blood lymphocyte subsets to aerosolized Pasteurella haemolytica A1

Jere McBride, Richard E. Corstvet, Bernadette C. Taylor, Bennie I. Osburn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Site-specific responses of bronchoalveolar and peripheral blood lymphocyte subsets were compared during primary and anamnestic immune responses against live Pasteurella haemolytica A1 (Ph1). Eight 1-year old calves were sequentially exposed intrabronchially with aerosolized Ph1 on days 0, 14, and 21, and two calves were sham exposed. Bronchoalveolar and peripheral blood lymphocytes were analyzed before each Phi exposure, and on days 3 and 7 post exposure using single and two-color flow cytometry to identify CD2, CD4+, CD8+, CD21+, CD45R+, CD25+ and γδ lymphocyte subsets. Significant differences (p < 0.05) in bronchoalveolar and peripheral blood lymphocyte subsets were observed before Ph1 exposure. Subsequent aerosol exposures, resulted in significant (p <0.05) changes in bronchoalveolar lymphocyte subsets and the CD4:CD8 bronchoalveolar lymphocyte ratio, but concomitant changes were not observed in peripheral blood lymphocytes. Expression of CD2, CD4 and CD8 lymphocyte differentiation antigens was consistently lower and more heterogeneous on bronchoalveolar lymphocytes. Differential analysis of bronchoalveolar leukocytes revealed a significant increase in bronchoalveolar lymphocytes and neutrophils during anamnestic responses.

Original languageEnglish (US)
Pages (from-to)161-170
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume67
Issue number2
DOIs
StatePublished - Feb 1 1999
Externally publishedYes

Fingerprint

Mannheimia haemolytica
Lymphocyte Subsets
lymphocytes
Lymphocytes
cattle
blood
Differentiation Antigens
Aerosols
differentiation antigens
calves
lymphocyte antigens
Flow Cytometry
Neutrophils
Leukocytes
Color
aerosols
flow cytometry
neutrophils
leukocytes
immune response

Keywords

  • Bovine
  • Bronchoalveolar
  • Lymphocyte
  • Pasteurella
  • Phenotype
  • Respiratory disease

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Primary and anamnestic responses of bovine bronchoalveolar and peripheral blood lymphocyte subsets to aerosolized Pasteurella haemolytica A1. / McBride, Jere; Corstvet, Richard E.; Taylor, Bernadette C.; Osburn, Bennie I.

In: Veterinary Immunology and Immunopathology, Vol. 67, No. 2, 01.02.1999, p. 161-170.

Research output: Contribution to journalArticle

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