Primary structure of a molluscan egg-specific NADase, a second-messenger enzyme.

D. L. Glick, Mark Hellmich, S. Beushausen, P. Tempst, H. Bayley, F. Strumwasser

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

An egg-specific NADase has been purified from the ovotestis of the marine mollusk Aplysia californica. The enzyme converts NAD to cyclic ADP-ribose (cADPR), which is a potent mobilizer of Ca2+. It is likely that the NADase serves to raise Ca2+ levels in the ova at appropriate times. A 1.2-kb cDNA clone containing the complete coding sequence of the native NADase protein was isolated from an unamplified ovotestis cDNA library and represents the first cloning of an NADase that generates cADPR. In vitro translation studies indicate that the protein initially has a signal sequence that may help to target it to discrete vesicles of the ova in which it is found. There are 12 cysteines in the open reading frame, two of these being in the signal sequence. No part of the sequence has significant similarity to other proteins or known nucleotide binding site consensus sequences. Northern blot analysis of poly(A)+ selected ovotestis RNA has identified an NADase mRNA of 1.85 kb. In situ hybridization analysis of cryostat sections from ovotestis has shown that the NADase mRNA is restricted to the immature ova, although the NADase protein is present in both immature and mature eggs.

Original languageEnglish (US)
Pages (from-to)211-218
Number of pages8
JournalCell regulation
Volume2
Issue number3
StatePublished - Mar 1991
Externally publishedYes

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NAD+ Nucleosidase
Second Messenger Systems
Ovum
Enzymes
Cyclic ADP-Ribose
Protein Sorting Signals
Proteins
Aplysia
Messenger RNA
Poly A
Mollusca
Consensus Sequence
Gene Library
Northern Blotting
NAD
Eggs
Open Reading Frames
In Situ Hybridization
Cysteine
Organism Cloning

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Glick, D. L., Hellmich, M., Beushausen, S., Tempst, P., Bayley, H., & Strumwasser, F. (1991). Primary structure of a molluscan egg-specific NADase, a second-messenger enzyme. Cell regulation, 2(3), 211-218.

Primary structure of a molluscan egg-specific NADase, a second-messenger enzyme. / Glick, D. L.; Hellmich, Mark; Beushausen, S.; Tempst, P.; Bayley, H.; Strumwasser, F.

In: Cell regulation, Vol. 2, No. 3, 03.1991, p. 211-218.

Research output: Contribution to journalArticle

Glick, DL, Hellmich, M, Beushausen, S, Tempst, P, Bayley, H & Strumwasser, F 1991, 'Primary structure of a molluscan egg-specific NADase, a second-messenger enzyme.', Cell regulation, vol. 2, no. 3, pp. 211-218.
Glick DL, Hellmich M, Beushausen S, Tempst P, Bayley H, Strumwasser F. Primary structure of a molluscan egg-specific NADase, a second-messenger enzyme. Cell regulation. 1991 Mar;2(3):211-218.
Glick, D. L. ; Hellmich, Mark ; Beushausen, S. ; Tempst, P. ; Bayley, H. ; Strumwasser, F. / Primary structure of a molluscan egg-specific NADase, a second-messenger enzyme. In: Cell regulation. 1991 ; Vol. 2, No. 3. pp. 211-218.
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